CN-121991800-A - Multi-temperature-zone independent temperature control system, method and medium for parallel biological reaction equipment

CN121991800ACN 121991800 ACN121991800 ACN 121991800ACN-121991800-A

Abstract

The invention provides a multi-temperature-zone independent temperature control system, a multi-temperature-zone independent temperature control method and a multi-temperature-zone independent temperature control medium for parallel biological reaction equipment, and relates to the field of parallel cell culture and high-flux biological reaction equipment temperature control. According to the invention, through the cooperative arrangement of the fans of the heating and refrigerating modules and the complementary control of heat, the mutual interference of different temperature areas on the ambient temperature is reduced, the overall heat exchange efficiency is improved, the device is suitable for parallel cell culture, orifice plate operation and high-flux experiment platforms, and the temperature control precision and experiment consistency are remarkably improved.

Inventors

WANG ZHONGZHOU
SUN HAIXUAN
LI JUNPO
WU SHENGPENG
YAN XUESONG
CAO RUNZI
DANG SHIJIE
XIAO DONGGEN

Assignees

中国科学院苏州生物医学工程技术研究所

Dates

Publication Date: 20260508
Application Date: 20260108

Claims (10)

1. A multi-temperature zone independent temperature control system for parallel biological reaction devices, comprising: the reactor temperature control module is used for providing temperature control for the bioreactor culture area; the sample operation temperature control module is used for providing temperature control for the pore plate or the sample operation area, and the sample operation temperature control module is physically separated from the reactor temperature control module; a control unit; the reactor temperature control module and the sample operation temperature control module each include: the Peltier temperature control unit is in communication connection with the control unit and is used for executing heating or refrigerating; The temperature sensor is arranged in the corresponding temperature zone, is used for detecting real-time temperature and is in communication connection with the control unit; The fan assembly is in communication connection with the control unit and is used for radiating the peltier temperature control unit; Wherein the control unit is configured to: Calculating temperature deviation based on the target temperature and the real-time temperature of each temperature zone; according to the temperature deviation, driving parameters of the Peltier temperature control units corresponding to the temperature areas are independently controlled; and according to the working state of each Peltier temperature control unit, the operation parameters of the corresponding fan assembly are independently controlled so as to realize independent and closed-loop temperature control of each temperature zone.
2. The multi-temperature zone independent temperature control system for parallel biological reaction devices of claim 1, further comprising a circulating water circuit assembly for remotely transferring heat between the peltier temperature control unit and the corresponding temperature zone to be controlled; the circulating waterway assembly comprises a diaphragm pump and a heat exchanger; The heat exchanger is respectively and thermally coupled with the heat exchange surface and the controlled temperature area of the Peltier temperature control unit; the diaphragm pump is connected with the control unit and used for driving the heat transfer medium to flow in the circulating waterway.
3. The multi-temperature zone independent temperature control system for a parallel biological reaction apparatus of claim 2, wherein the control unit is further configured to perform cooperative control in a remote heat transfer mode such that: Heat exchange power generated by the peltier temperature control unit Effective heat exchange power transferred by the circulating waterway And the heat load requirement corresponding to the temperature controlled area The following relationships are satisfied: ; At the same time, controlling the heat dissipation capacity of the fan assembly So that it satisfies the following conditions: 。
4. A multi-temperature zone independent temperature control system for parallel biological reaction devices according to claim 1 or 3, characterized in that the control unit is configured to implement a thermal complementation strategy: and the temperature control module of the reactor and the fan assembly of the sample operation temperature control module are cooperatively arranged and regulated, so that the air flow exhausted by the fan of one temperature control module at least partially acts on the Peltier temperature control unit of the other temperature control module, and the influence of environmental temperature disturbance on different temperature areas is reduced.
5. The multiple temperature zone independent temperature control system for parallel biological reaction devices of claim 4 wherein the thermal complementation strategy satisfies the following constraints: Wherein, the And The heat dissipation capacity is designed for the fans of the heating module and the cooling module respectively, And The power of the heating module peltier power and the power of the refrigerating module peltier power are respectively, And The heat exchange efficiency coefficients of the heating module and the refrigerating module are respectively, Is an environmental heat influence cancellation coefficient.
6. A multi-temperature zone independent temperature control method for parallel biological reaction devices, applied to the system according to any one of claims 1 to 5, comprising the steps of: Setting target temperatures of the temperature areas respectively; respectively collecting real-time temperature of each temperature zone; Calculating independent temperature deviation of each temperature zone according to the target temperature and the real-time temperature of each temperature zone; According to the temperature deviation of each temperature zone, driving voltage and current of the corresponding Peltier temperature control unit are independently controlled to execute heating or refrigerating operation; and according to the working state and power of each Peltier temperature control unit, the operation parameters of the corresponding fan assembly are independently regulated so as to manage heat dissipation.
7. The multi-temperature zone independent temperature control method for a parallel biological reaction apparatus according to claim 6, wherein when the circulation waterway is used for remote heat transfer, the method further comprises: According to the heat load demand of the controlled temperature zone And the temperature deviation is used for determining the heat exchange power required to be transmitted by the circulating waterway ; The rotating speed of the diaphragm pump is regulated to control the flow of the circulating medium, so that the actual waterway heat exchange power approaches the heat exchange power ; Adjusting driving parameters of the Peltier temperature control unit to generate heat exchange power Satisfy the following requirements ; Adjusting the operation parameters of the fan assembly to enable the fan assembly to have heat dissipation capacity Satisfy the following requirements 。
8. The multi-temperature zone independent temperature control method for a parallel biological reaction apparatus of claim 6, further comprising a thermal complementation control step of: monitoring whether each peltier temperature control unit is in a heating mode or a cooling mode; According to the power and position relation between the heating module and the refrigerating module, the wind direction or the rotating speed of the corresponding fan is dynamically adjusted, so that low-temperature airflow generated by the heating module fan is led to the hot end area of the refrigerating module, and the thermal interference of the refrigerating module to the environment is partially counteracted.
9. The multi-temperature zone independent temperature control method for parallel biological reaction apparatuses according to claim 6, wherein the target temperature of the reactor culture zone is set in a physiologically relevant range of 20 ℃ to 40 ℃, and the target temperature of the sample operation zone is independently set according to experimental requirements and is different from the target temperature.
10. A computer readable storage medium, on which a computer program is stored, characterized in that the computer program, when being executed by a processor, implements the steps of the method according to any one of claims 6-9.

Description

Multi-temperature-zone independent temperature control system, method and medium for parallel biological reaction equipment Technical Field The invention relates to the technical field of cell culture and high-flux parallel biological reaction equipment, in particular to a multi-temperature-zone independent temperature control system, a multi-temperature-zone independent temperature control method and a multi-temperature-zone independent temperature control medium for parallel biological reaction equipment. Background Along with the development of biopharmaceutical and regenerative medicine research, the high-throughput multichannel parallel culture platform is increasingly widely applied to process development, parameter screening and process amplification verification. Cell culture has high stability and consistency requirements for culture environments (especially temperature, dissolved oxygen, pH and shear force), and in high throughput parallel systems, different temperature zones often have different target temperatures, for example, the reactor culture zone needs to maintain physiological temperature (e.g., 37 ℃) while the well plate sample operating zone needs to have lower or adjustable temperature to keep reagents or samples stable. The common temperature control method in the prior art is mainly centralized constant temperature, local water bath or Peltier (thermoelectric) control in a single area. Although the peltier module is used for miniaturization and modularized temperature control and can realize rapid cooling/heating, when a plurality of temperature areas are actually integrated and a certain physical distance exists between a temperature control unit and a controlled area, the direct thermal coupling efficiency is reduced, so that the following technical problems are not solved well: 1. thermal crosstalk in multiple temperature areas, namely heat flow/cold flow generated by heating or refrigerating in different temperature areas, are mutually influenced through air convection or structural conduction, so that real independent control is difficult to realize, and culture consistency and data comparability are influenced. 2. The remote heat transfer efficiency is limited, when the peltier module cannot be directly and tightly attached to a controlled area, an intermediary (such as a circulating waterway) is needed to transfer heat, and if the circulating parameters are not matched with the peltier power and the fan heat dissipation capacity, temperature control lag or energy waste can occur. 3. The problem of environmental disturbance and heat dissipation, namely the Peltier, can generate larger heat flux density at the hot end/cold end in the refrigerating or heating process, and if the arrangement and heat exchange capacity of the fan are not optimized, the temperature fluctuation of the surrounding environment can be caused, so that the precision culture is not facilitated. 4. The control strategy lacks quantitative matching, the existing system depends on empirical parameters or a single control loop (such as simple PID), and the engineering control scheme and method support for quantitatively coupling the Peltier electric parameters, the circulating waterway flow and the fan heat exchange capacity are lacking. Therefore, it is necessary to provide a comprehensive temperature control system and method for multiple temperature areas and considering the remote heat transfer situation, which not only ensure independent closed-loop control of each temperature area, but also optimize heat flow and dynamic coordination among a diaphragm pump circulating waterway, a peltier module and a fan through engineering mathematical matching so as to improve the temperature control response speed, stability and energy efficiency, and reduce disturbance to the environment. Disclosure of Invention To achieve the above objects and other advantages and in accordance with the purpose of the invention, a first object of the invention is to provide a multi-temperature zone independent temperature control system for a parallel biological reaction apparatus, comprising: the reactor temperature control module is used for providing temperature control for the bioreactor culture area; the sample operation temperature control module is used for providing temperature control for the pore plate or the sample operation area, and the sample operation temperature control module is physically separated from the reactor temperature control module; a control unit; the reactor temperature control module and the sample operation temperature control module each include: the Peltier temperature control unit is in communication connection with the control unit and is used for executing heating or refrigerating; The temperature sensor is arranged in the corresponding temperature zone, is used for detecting real-time temperature and is in communication connection with the control unit; The fan assembly is in communication connec