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CN-121991810-A - Microbial agent fermented by traditional Chinese medicine residues and preparation method and application thereof

CN121991810ACN 121991810 ACN121991810 ACN 121991810ACN-121991810-A

Abstract

The invention provides a microbial agent fermented by utilizing Chinese medicinal residues, and a preparation method and application thereof, and relates to the technical field of soil improvement. The microbial agent is mainly prepared by fermenting a Chinese medicinal residue fermentation substrate by trichoderma brevicompactum, wherein the effective viable count of the trichoderma brevicompactum in the microbial agent is more than or equal to 0.2 multiplied by 10 8 CFU/g, and the moisture content is less than or equal to 30%. The invention creatively utilizes the Chinese medicinal residues and the furfural residues as matrixes, and prepares the high-activity microbial agent through the optimized fermentation of specific trichoderma brevicompactum. The microbial inoculum successfully realizes the production of treating waste with waste and has low cost, and surprisingly discovers that the microbial inoculum has a synergistic effect in improving the saline-alkali soil and simultaneously improving the emergence rate, the seedling storage rate and the growth promotion of safflower under the saline-alkali stress.

Inventors

  • WANG QIUXIA
  • MA XIAOLIN
  • CHEN SHAOHUA
  • ZHANG LINLIN
  • WANG WENJIAN
  • GAO MIN
  • SHAO CAI
  • LIU HUITAO

Assignees

  • 吉林万通集团盛泰生物工程股份有限公司
  • 中国农业科学院特产研究所

Dates

Publication Date
20260508
Application Date
20260210

Claims (10)

  1. 1. The microbial agent is characterized by being prepared by fermenting a traditional Chinese medicine residue fermentation substrate through trichoderma brevicompactum (Trichoderma brevicompactum), wherein: The effective viable count of the trichoderma brevicompactum in the microbial agent is more than or equal to 0.2 multiplied by 10 8 CFU/g, and the moisture content is less than or equal to 30%.
  2. 2. The microbial agent for fermentation by using Chinese medicinal residues according to claim 1, wherein the trichoderma brevicompactum is trichoderma brevicompactum strain Tri502; The trichoderma brevicompactum strain Tri502 is preserved in China general microbiological culture Collection center (CGMCC) at 8 and 19 days of 2021, and has the preservation number of CGMCC No.23213, and the preservation date of 2021, 8 and 19 days, and the preservation address is North Star Xway No. 1, 3 in the Korean region of Beijing.
  3. 3. The microbial agent for fermentation using Chinese medicinal residues according to claim 1, wherein the Chinese medicinal residue fermentation substrate comprises Chinese medicinal residues and furfural residues; preferably, the sources of the traditional Chinese medicine residues are residues generated by Wantong bone and muscle tablets and Wantong bone and muscle paste products produced by Jilin Wantong pharmaceutical industry groups.
  4. 4. The microbial agent for fermentation by utilizing Chinese medicinal residues according to any one of claims 1 to 3, wherein the microbial agent is prepared by fermenting the following raw materials in percentage by weight: 55-65% of Chinese medicine residues, 30-45% of furfural residues and 0.5-1.5% of trichoderma brevicompactum liquid.
  5. 5. A method for preparing a microbial agent by fermenting Chinese medicinal residues according to any one of claims 1 to 4, wherein the method comprises the following steps: inoculating trichoderma brevicompactum into a traditional Chinese medicine residue fermentation matrix for solid state fermentation, drying and crushing a product after the solid state fermentation until the moisture content is less than or equal to 30%, and obtaining a product A; detecting the effective viable count of the trichoderma brevicompactum in the product A, and obtaining the microbial agent when the effective viable count of the trichoderma brevicompactum is more than or equal to 0.2 multiplied by 108 CFU/g.
  6. 6. The method for preparing microbial inoculum by utilizing traditional Chinese medicine residue fermentation according to claim 5, which is characterized in that the temperature of the solid state fermentation is controlled at 25-35 ℃; Preferably, the fermentation temperature is maintained at 28-32 ℃ through turning and ventilation; preferably, the solid state fermentation time is 7-15 days.
  7. 7. The method for preparing microbial inoculum by fermentation of Chinese medicinal residues according to claim 5, further comprising a step of pretreatment of the Chinese medicinal residue fermentation substrate before inoculation.
  8. 8. The method for preparing microbial agent by fermentation of Chinese medicinal residues according to claim 7, wherein the method for pretreating the Chinese medicinal residue fermentation substrate comprises: and (3) treating the Chinese medicinal residues to control the water content to be 40-60%, adjusting the pH to be 6-7, and then adding furfural residues into the Chinese medicinal residues to adjust the carbon-nitrogen ratio of the materials to be 20-30:1.
  9. 9. The method for preparing microbial agent by utilizing fermentation of Chinese medicinal residues according to claim 5, wherein the drying temperature is 30-33 ℃; Preferably, the particle size of the crushing is 1-3 mm.
  10. 10. The application of the microbial agent fermented by traditional Chinese medicine residues according to any one of claims 1-4 in the preparation of the following products: preparing a saline-alkali soil improvement product; Preparing a product for improving the emergence rate of safflower (Carthamus tinctorius L.) under the stress of salt and alkali; preparing a product for improving the survival rate of safflower (Carthamus tinctorius L.) under the stress of salt and alkali; A product is prepared that promotes the growth of safflower (Carthamus tinctorius l.) under salt and alkali stress.

Description

Microbial agent fermented by traditional Chinese medicine residues and preparation method and application thereof Technical Field The invention relates to the technical field of soil improvement, in particular to a microbial agent fermented by traditional Chinese medicine residues, and a preparation method and application thereof. Background Soil salinization is one of the major abiotic stress factors affecting global agricultural production and ecological environment. Salinized soil can produce negative effects such as osmotic stress, ion toxicity and nutrition imbalance on plants, so that germination of seeds, survival of seedlings and growth and development of the plants are seriously inhibited, and finally, crops are greatly reduced in yield. To improve and utilize saline-alkali soil, various methods have been explored, mainly including physical improvement, chemical improvement and biological improvement methods. Among them, biological improvement is currently considered to be the most sustainable approach, and soil improvement by using microbial agents is a research hotspot. Trichoderma (Trichoderma spp.) is known as a common biocontrol fungus and has the effects of antagonizing pathogenic bacteria, inducing systemic resistance of plants, promoting plant growth, etc. Trichoderma has been studied for controlling plant diseases and promoting growth. However, the application of trichoderma agents to saline-alkali soil improvement, particularly in overcoming saline-alkali stress to increase crop emergence rates, still presents a number of challenges and limitations, particularly as follows: 1. The microbial inoculum has poor adaptability, a plurality of commercial trichoderma reagents are not developed for the saline-alkali soil environment with high salt and high pH, the survival rate of the strain in the saline-alkali soil is low, the colonization capability is weak, and the actual effect is unstable or even ineffective. 2. The production cost contradicts with the recycling, the cost of the traditional trichoderma agent culture matrix (such as bran, corn flour and the like) is higher, so that the application economy of the trichoderma agent culture matrix in the improvement of large-area saline-alkali soil is insufficient. Meanwhile, the disposal of a large amount of organic waste such as chinese medicine residues and furfural residues, which are generated in industrial and agricultural production, has become a heavy environmental burden. The Chinese medicine dregs have huge annual discharge amount, are rich in organic matters such as cellulose, and the furfural dregs are acidic and contain rich organic components. Although they are theoretically available as resources, the current methods for recycling them are inefficient, and most of them are mainly filled or discarded, and high-value conversion cannot be achieved. Therefore, the technical scheme with low cost, environmental friendliness and remarkable effect is urgently needed in the field, industrial and agricultural wastes can be effectively utilized, and the special microbial agent which is suitable for saline-alkali soil conditions and can remarkably improve the crop emergence rate is produced. In view of this, the present invention has been made. Disclosure of Invention The invention aims to provide a microbial agent for fermenting traditional Chinese medicine residues, which can effectively improve saline-alkali soil, and simultaneously improve the emergence rate, the seedling storage rate and the growth promotion of safflower under saline-alkali stress. The second aim of the invention is to provide a preparation method of microbial agent by utilizing fermentation of traditional Chinese medicine residues. The third purpose of the invention is to provide an application of a microbial agent by utilizing fermentation of traditional Chinese medicine residues. In order to achieve the above object of the present invention, the following technical solutions are specifically adopted: the invention provides a microbial agent fermented by traditional Chinese medicine residues, which is mainly prepared by fermenting a traditional Chinese medicine residue fermentation substrate by trichoderma brevicompactum (Trichoderma brevicompactum), wherein: The effective viable count of the trichoderma brevicompactum in the microbial agent is more than or equal to 0.2 multiplied by 10 8 CFU/g, and the moisture content is less than or equal to 30%. Further, the trichoderma brevicompactum is trichoderma brevicompactum strain Tri502; The trichoderma brevicompactum strain Tri502 is preserved in China general microbiological culture Collection center (CGMCC) at 8 and 19 days of 2021, and has the preservation number of CGMCC No.23213, and the preservation date of 2021, 8 and 19 days, and the preservation address is North Star Xway No. 1, 3 in the Korean region of Beijing. Further, the traditional Chinese medicine residue fermentation substrate comprises traditional Chinese