CN-121991811-A - Nicotine tearing resistant harrow tooth bacterium IL01 and application thereof

Abstract

The invention discloses a nicotine-resistant harrow tooth bacterium IL01 and application thereof, belonging to the technical field of agricultural microorganisms. The invention provides a tobacco source strain with fermentation characteristics of nicotine resistance, salt resistance, acid resistance, high-yield lignocellulose activity, aroma production and the like, which is identified as white rot fungi, namely the harrow teeth are used for fermenting different substrates to prepare tobacco spice, so that the sensory quality of the cigarette can be improved after the cigarette is flavored, and tobacco source lignocellulose such as stem paste, stem shreds, tobacco leaves, tobacco dust, tobacco soil rods or tobacco straws and the like can be utilized in a high value to be converted into flavor substances. The invention can provide a new technical approach for improving the quality of cigarette products and reducing the cost, provides a new idea for the utilization of tobacco biomass resources in the future, accords with the development concept of double carbon, and has important significance for promoting the development of green low carbon in agriculture and comprehensively realizing village plain.

Inventors

• ZHAO YUZONG
• FANG SONG
• QIU JUN
• SONG KAIHUI
• LIU JINJIN

Assignees

• 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所)

Dates

Publication Date

20260508

Application Date

20260127

Claims (9)

1. 1. A strain of Rake lachrymatory bacterium IL01 is characterized in that the class of the Rake lachrymatory bacterium IL01 is Irpex LACERATES IL01, and the Rake lachrymatory bacterium IL01 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20241403.
2. 2. The harrow tooth bacteria IL01 according to claim 1, wherein the harrow tooth bacteria IL01 is obtained by screening tobacco leaves, and has the fermentation characteristics of high salt resistance, high acid resistance, high nicotine resistance, high lignin degradation enzyme activity yield, qualitative aroma production and high extracellular polysaccharide yield.
3. 3. An article of manufacture prepared using the Rake teardrop IL01 of claim 1.
4. 4. The article of claim 3, wherein the article comprises a tobacco flavor.
5. 5. Use of the harrow tooth bacterium IL01 according to any one of claims 1 to 2 for the high-value utilization of tobacco-derived lignocellulose.
6. 6. The use according to claim 5, wherein the tobacco-derived lignocellulose comprises any one or more of stem paste, cut stems, tobacco leaves, tobacco dust, tobacco rods or tobacco stalks.
7. 7. Use of the harrow tooth bacterium IL01 according to any one of claims 1-2 for the preparation of tobacco products.
8. 8. The use of claim 7, wherein the tobacco product comprises a tobacco flavor.
9. 9. The use according to claim 8, wherein the tobacco flavor has a floral, cheese, fruit, floral, baking and/or cocoa flavor.

Description

Nicotine tearing resistant harrow tooth bacterium IL01 and application thereof Technical Field The invention relates to a nicotine-resistant harrow tooth bacterium and application thereof, belonging to the technical field of agricultural microorganisms. Background Tobacco is used as a global important economic crop, and a large amount of tobacco source waste such as tobacco stems, tobacco dust, crushed tobacco flakes, partial low-grade tobacco leaves and the like can be generated in the processing process. The wastes have complex chemical structures and are rich in cellulose, hemicellulose, lignin and other components, and are an important source of tobacco lignocellulose. Research has shown that the types of components contained in tobacco lignocellulose are basically consistent with the components of tobacco leaves, so that the tobacco lignocellulose has wide application in cigarette production. At present, tobacco source lignocellulose is an essential important raw material of cigarette products, but the content of the flavor components of the tobacco source lignocellulose is still greatly different from that of natural tobacco leaves, and the tobacco source lignocellulose is also an important reason for the difference between the sensory quality of the tobacco source lignocellulose and the natural tobacco leaves. The reason for the difference of the fragrance components is mainly that the base of the raw materials is poor, and the fragrance components are lost in a large amount after the processes of extraction, concentration, high-temperature baking and the like, so that the fragrance is flat and the woody miscellaneous gas is heavy. In addition, tobacco lignocellulose contains macromolecular substances such as proteins and pectins which have a great influence on sensory quality. These problems limit the use of tobacco-derived lignocellulose in cigarette products. At present, the utilization of tobacco lignocellulose is in a primary stage and is mainly limited by the characteristic of difficult degradation and the environment with high nicotine and high osmotic pressure, so that the degradation activity of conventional microorganisms is obviously inhibited, the degradation rate is low, the period is long, and the industrialized application is restricted. Therefore, the development of the microorganism strain capable of efficiently degrading the tobacco lignocellulose and the application of the microorganism strain to the high-value utilization of the tobacco lignocellulose have important significance. The application of microorganism strain in tobacco has been reported, and in patent application of CN202410041946.6, the inventor utilizes a strain of smoke alkali cellulose degradation resistant bacteria-Acinetobacter indicum LXX-2022-12 for degrading cellulose in tobacco straw. In the patent application of CN202311466890.0, the inventor utilizes a lignin degrading bacterium YP-2023-9 resistant to nicotine to degrade lignin in tobacco stems, and the strain can grow on the epidermal tissue of the tobacco stems, so that the tissue structure of the tobacco stems is loose and easy to degrade. In the patent application of CN202410909350.3, the inventor utilizes a low-temperature-resistant and nicotine-resistant Bacillus sp.YP 202310 strain for degrading tobacco straws, and the strain can stably grow in a field environment at 15-35 ℃ so as to provide a promoting effect for degradation after the flue-cured tobacco straws are directly returned to the field. In the patent application of CN202310589377.4, the inventor utilizes a pantoea B-3 screened from cigars to degrade cellulose in tobacco, and metabolic enzyme products of the pantoea B-3 can reduce the cellulose content in tobacco, improve the irritation and bad smell of the tobacco in the burning and sucking process and improve the sucking safety of the tobacco. The efficient conversion of the lignocellulose full component is a key for improving the added value of tobacco lignocellulose such as tobacco straw and the like, but the prior art is still immature, and the problems of insufficient strain tolerance and difficult degradation of lignocellulose are difficult to synchronously solve. In the patent application of CN201410039634.8, although the degradation efficiency of nicotine can be improved by 60% -78% by utilizing a strain of Arthrobacter hispidus, the degradation of lignocellulose still needs to depend on the synergistic effect of compound flora. In addition, in the existing research reports about application of microbial strains to tobacco lignin, strains with nicotine, salt and acid resistance and simultaneously capable of degrading lignocellulose and improving aroma quality are not searched. Based on the method, the white rot fungus tearing harrow fungus resistant to nicotine is obtained from tobacco leaves through screening, and has been reported in high-value utilization of agricultural wastes, and the white rot fungus tearing harrow fungus has g