CN-121991814-A - Plastic film fungus strain and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and discloses a strain of glue film fungus (Tulasnella sp.) and application thereof. The preservation number of the strain is CGMCC NO.42499. The strain has remarkable promotion effect on seed germination, seedling growth, seedling rooting and accumulation of polysaccharide content in stems of dendrobium candidum and dendrobium huoshanense.

Inventors

• CHEN JUAN
• Leng Chunyan
• HUANG XINJIE
• WANG AIYUAN
• XING YONGMEI

Assignees

• 中国医学科学院药用植物研究所

Dates

Publication Date

20260508

Application Date

20260205

Claims (7)

1. 1. The strain C0337 of the glue film fungus (Tulasnella sp.) is characterized in that the preservation number of the strain is CGMCC No. 42499.
2. 2. The glue film fungus strain C0337 according to claim 1, wherein rDNA-ITS sequence of the strain C0337 is shown in SEQ ID NO. 1.
3. 3. A biological agent comprising the strain C0337 of the embrocation reesei of claim 1 or 2.
4. 4. A method for promoting germination, tillering, rooting or accumulation of polysaccharide content in seeds of a dendrobium (Dendrobium) plant, characterized in that it comprises treating the seeds or seedlings of a dendrobium plant with a glue film strain C0337 according to claim 1 or 2 or a biological agent according to claim 3.
5. 5. The method of claim 4, wherein the dendrobe plant is any species of dendrobe.
6. 6. The method of claim 5, wherein the dendrobe is dendrobium candidum (d. Ofacinale) or dendrobium huoshanense (d. Huoshanense).
7. 7. Use of the strain C0337 of the embrocation of claim 1 or 2 or the biological agent of claim 3 in the following: 1) Promoting germination of seeds of dendrobium plants; 2) Promoting tillering of seedlings of the dendrobium plants; 3) Promoting rooting of seedlings of Dendrobium plants, or 4) Promoting polysaccharide content accumulation of Dendrobium plant.

Description

Plastic film fungus strain and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a glue film fungus strain for promoting germination of dendrobium seeds, growth of seedlings, rooting of seedlings and accumulation of polysaccharide content and application thereof. Background Dendrobium (Dendrobium) belongs to the genus Dendrobium of the family Orchidaceae, which is one of the largest genus of orchid, and is widely distributed in east Asia, south Asia and southeast Asia, and has about 1800 times of the whole world. In China, dendrobium is mainly distributed in Qinling mountain in the south province. A variety of plants of the genus Dendrobium can be used as medicinal plants, such as Dendrobium officinale. The Chinese pharmacopoeia (2025 edition) records that Dendrobium officinale has the effects of benefiting stomach, promoting fluid production, nourishing yin and clearing heat, and can be used for treating symptoms such as heat disease, fluid injury, yin deficiency, fire excess, bone steaming, fatigue and fever and the like. Modern researches show that Dendrobium officinale contains polysaccharide, stilbene, flavonoid, alkaloid and other components, has the functions of resisting oxidation and aging, regulating gastrointestinal tract, regulating immunity, resisting tumor and other pharmacological actions, is an important medicinal plant resource in China, and has great medicinal value and economic value. Along with the promotion of people's health consciousness and the development of traditional Chinese medicine industry, the market demand of dendrobium candidum is continuously increased. However, dendrobium candidum has strict requirements on habitat conditions, low natural reproduction rate and long-term excessive digging, and wild resources are in endangered states. At present, the market supply of dendrobium candidum mainly depends on artificial cultivation, but the technical bottlenecks of unstable quality, difficult yield improvement and the like still exist in the artificial cultivation process. In natural conditions, seed germination and seedling growth development of orchids depend on the particular symbiotic fungus. Symbiotic fungi can provide needed nutrition for growth and development, regulate growth and influence secondary metabolism, and are important biological factors for realizing population updating and breeding of orchid plants. Therefore, the fungus strain which can be cultured and can establish stable symbiotic relation with dendrobium candidum seedlings is screened, and the method has important significance for solving the problems of cultivation efficiency and quality control in the prior art, improving the artificial cultivation efficiency of dendrobium candidum and ensuring the cultivation quality. Disclosure of Invention Accordingly, the invention aims to provide a glue film fungus (Tulasnella sp.) strain C0337 capable of promoting germination of dendrobium seeds, tillering of seedlings and accumulation of polysaccharide content, so as to solve the problem of poor cultivation efficiency and quality control of dendrobium plants in the prior art, and provide effective options for improving the artificial cultivation efficiency of dendrobium and guaranteeing the cultivation quality. In one aspect, the invention provides a strain of plasmopara (Tulasnella sp.) C0337. The strain is separated from dendrobium huoshanense root in Li An Huoshan county of Anhui 11/15/2022, and is preserved in China general microbiological culture Collection center (CGMCC) in 01/06/2026, the preservation number is CGMCC No. 42499, the taxonomy of the strain C0337 is named as glue film bacteria, and the Latin literature is named as Tulasnella sp. In an embodiment of the first aspect, rDNA-ITS sequence of the Tamarix strain C0337 is shown in SEQ ID NO. 1. In an embodiment of the first aspect of the invention, the strain of Emblica officinalis C0337 has the morphological characteristics that on PDA, the colony of the strain of Emblica officinalis C0337 is white, circular, a small amount of aerial hyphae grows, the colony diameter of 6 days at 25 ℃ is 5.00 cm, and no spores are produced on PDA. In a second aspect, the invention provides a biological agent comprising strain C0337. The biological agent can promote seed germination, seedling tillering, seedling rooting and polysaccharide content accumulation of dendrobium plants. In a third aspect, the invention provides a method of promoting germination of seeds, tillering of seedlings, rooting of seedlings or accumulation of polysaccharide content of dendrobe plants, comprising treating seeds or seedlings of dendrobe plants with a strain of shelf fungus C0337 or a biological agent according to the second aspect. In an embodiment of the third aspect, the treatment may be, for example, inoculating the dendrobe strain C0337 or the biological agent of the second aspect to dendrobe seeds or s