Search

CN-121991816-A - Composite microbial inoculum for dairy wash water treatment, method and application thereof

CN121991816ACN 121991816 ACN121991816 ACN 121991816ACN-121991816-A

Abstract

The invention belongs to the technical field of resources and environmental microorganisms, and discloses a composite microbial agent for treating milk washing water, a method and application thereof, wherein the composite microbial agent comprises Kluyveromyces marxianus ART-27 and lactobacillus fermentum. The lactobacillus fermentum and the kluyveromyces marxianus adopted by the invention are edible safe strains, and have higher biocompatibility and application safety. Under the culture condition of low concentration lactose and protein, the compound bacteria system shows remarkable synergistic effect, the OD 600 value reaches 2.43, and compared with single strain lactobacillus and saccharomycete fermentation of a control group, 76.17 percent and 51.02 percent are respectively improved. The complex bacteria fermentation simulated water can utilize 81% lactose and 94.26% protein. The composite bacteria can ferment the actual flushing water to effectively reduce COD, TN and SS of the actual flushing water, and the removal rates respectively reach 78.23%, 80.67% and 84.09%.

Inventors

  • WANG MIN
  • SHEN YANBING
  • WU YUHAN
  • MA AIJING
  • SU ZHENHUA

Assignees

  • 天津科技大学

Dates

Publication Date
20260508
Application Date
20260409

Claims (10)

  1. 1. A composite microbial inoculum for dairy wash water treatment is characterized by comprising Kluyveromyces marxianus (Kluyveromyces marxianus) ART-27 and lactobacillus fermentum (Lactobacillus fermentum); Wherein, the classification name of the Kluyveromyces marxianus ART-27 is Kluyveromyces marxianus (Kluyveromyces marxianus), the preservation number is CGMCC No.39119, the preservation date is 2026, 1 month and 7 days, and the preservation unit is China general microbiological culture Collection center (CGMCC for short), the Beijing Korea North Star Xiu No. 1, 3.
  2. 2. The composite microbial inoculum according to claim 1 wherein the Lactobacillus fermentum comprises Lactobacillus fermentum AF 7-2, lactobacillus fermentum AF1-3, lactobacillus fermentum AF1-5, lactobacillus fermentum ATCC 9338, lactobacillus fermentum CECT5716.
  3. 3. The composite microbial agent according to claim 1 or 2, wherein the volume ratio of lactobacillus fermentum to kluyveromyces marxianus ART-27 is 1:0.5-1:3.
  4. 4. Use of a complex microbial agent according to any one of claims 1 to 3 for the treatment of low concentration dairy water.
  5. 5. The method according to claim 4, wherein the complex bacteria utilize lactose and protein in low concentration milk wash water by fermentation; Or the milk water comprises milk waste water, milk pipeline flushing water, milk tank flushing water, milk water and the like.
  6. 6. The method for preparing the composite microbial inoculum according to any one of claims 1 to 3, which is characterized by comprising the following steps: (1) Respectively preparing lactobacillus fermentum seed solution and Kluyveromyces marxianus ART-27 seed solution; (2) Washing the two seed solutions in the step (1) with 10 XPBS, repeating for three times, and adjusting to a bacterial suspension with OD 600 of 1; (3) Mixing the two bacterial suspensions obtained in the step (2) according to the volume ratio of 1:0.5-1:3 to prepare a composite bacterial suspension, thereby obtaining the composite bacterial agent.
  7. 7. The method of claim 6, wherein in the step (1), the Lactobacillus fermentum seed solution is prepared by streaking and activating the strain, selecting single colony, inoculating into MRS liquid culture medium, and culturing at 37deg.C under static condition for 12-14 h.
  8. 8. The method according to claim 6 or 7, wherein in the step (1), the Kluyveromyces marxianus ART-27 seed solution is prepared by streaking and activating a strain of Kluyveromyces marxianus ART-27, picking a single colony, inoculating the single colony into YPD liquid culture medium, and standing and culturing at 30 ℃ for 16-18 h; Or in the step (3), the bacteria are mixed according to the volume ratio of 1:1 to prepare the composite bacterial suspension.
  9. 9. A method of treating low concentration dairy rinse water with a complex microbial inoculant according to any one of claims 1 to 3, comprising the steps of: inoculating the composite microbial inoculum into low-concentration dairy wash water to be treated for fermentation treatment.
  10. 10. The method of claim 9, wherein the total inoculum size of the composite microbial inoculum is 0.5-7% of the volume of the low-concentration dairy rinse water.

Description

Composite microbial inoculum for dairy wash water treatment, method and application thereof Technical Field The invention belongs to the technical field of resources and environmental microorganisms, and particularly relates to a composite microbial inoculum for dairy wash water treatment, a method and application thereof. Background The dairy industry generates a large amount of process water in the production process, about 400 to 1100 ten thousand tons of dairy waste are discharged into the environment every year worldwide, and the daily waste forms a serious threat to biodiversity. It is counted that about 6 to 10 liters of wastewater is produced per one liter of milk processed. The main components of the wastewater are low-concentration protein, lactose, inorganic salt, nitrogen, phosphorus and other nutrient substances, and the wastewater has the characteristics of large water discharge amount and rich organic components. Under the background of current shortage of water resources and increasingly strict environmental protection requirements, how to effectively treat dairy waste water and realize recycling has become a problem to be solved urgently in the industry. At present, the mode of mixed treatment of waste water in each working section is generally adopted for physical treatment of milk product cleaning waste water. The mode of 'mixing treatment-up to standard discharge' mixes high-quality flushing water rich in lactose and protein with other wastewater, so that nutrition resources are diluted and wasted, recovery value is lost, and energy consumption and cost of terminal treatment are increased. If the wastewater treatment can be changed from single degradation and removal to recycling and value-added, a new path with environmental protection and economic benefits is provided for the dairy industry. A plurality of bacteria (such as bacillus cereus, bacillus subtilis, escherichia coli and the like) can be naturally separated from the dairy waste water, and microorganisms such as staphylococcus aureus, fusarium, aspergillus and the like can be obtained through culture, and the flora has a certain effect on reducing the organic load of the waste water. However, existing bacterial agents are not generally listed in edible fungus lists, have poor activity in low-concentration wastewater, and have uncertainty in fermentation metabolites, so that the available ways of the treated recycled water are limited. The method is limited by the characteristic of low concentration of organic matters in milk washing water, the utilization capacity and metabolic capacity of single microorganism on milk water components are limited, and the bottleneck that single microorganism grows slowly and has low utilization rate under low concentration substrate is hopeful to be solved through metabolic complementation, synergy and ecological niche differentiation of double-microorganism mixed fermentation. Therefore, constructing the composite strain for efficiently utilizing the low-concentration dairy component is one of important subjects for solving the application of the dairy water resource, and has important practical value and academic research value. Therefore, the dairy waste water is efficiently degraded through the edible fungus group so as to widen the utilization way of the reclaimed water, which is a technical problem to be solved currently urgently, and has important research value and application prospect in the fields of industrial waste water treatment and resource utilization. Disclosure of Invention The invention aims to overcome the defects in the prior art and provides a composite microbial inoculum for dairy water treatment, a method and application thereof. A complex microbial inoculant for dairy rinse water treatment, the complex microbial inoculant comprising kluyveromyces marxianus (Kluyveromyces marxianus) ART-27 and lactobacillus fermentum (Lactobacillus fermentum); Wherein, the classification name of the Kluyveromyces marxianus ART-27 is Kluyveromyces marxianus (Kluyveromyces marxianus), the preservation number is CGMCC No.39119, the preservation date is 2026, 1 month and 7 days, and the preservation unit is China general microbiological culture Collection center (CGMCC for short), the Beijing Korea North Star Xiu No. 1, 3. Further, the lactobacillus fermentum comprises lactobacillus fermentum AF 7-2, lactobacillus fermentum AF1-3, lactobacillus fermentum AF1-5, lactobacillus fermentum ATCC 9338, lactobacillus fermentum CECT5716 and the like; Further, the volume ratio of the lactobacillus fermentum to the Kluyveromyces marxianus ART-27 is 1:0.5-1:3, preferably 1:1. The application of the composite microbial inoculum in treating low-concentration dairy water. Further, the complex bacteria can utilize lactose and protein in low concentration dairy wash water by fermentation; Or the milk water comprises milk waste water, milk pipeline flushing water, milk tank flushing water and milk water. The