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CN-121991818-A - Kluyveromyces marxianus, method and application thereof

CN121991818ACN 121991818 ACN121991818 ACN 121991818ACN-121991818-A

Abstract

The invention belongs to the technical field of resource and environmental microorganisms, and discloses Kluyveromyces marxianus, a method and application thereof, wherein the name of the Kluyveromyces marxianus is ART-27, the classification name of the Kluyveromyces marxianus is Kluyveromyces marxianus (Kluyveromyces marxianus), the preservation number of the Kluyveromyces marxianus is CGMCC No.39119, and the preservation date of the Kluyveromyces marxianus is 2026, 1 month and 7 days. The yeast provided by the invention can flocculate macromolecular proteins in dairy water and improve the utilization rate of lactose and proteins by utilizing lactose, protein and other components, wherein the degradation rate of the Kluyveromyces marxianus on lactose is 70.20%, which is improved by 20.20% compared with the degradation rate of the original yeast by 50%, and the degradation rate of the lactalbumin is 76.20%, which is improved by 22.2% compared with the degradation rate of the original yeast by 54%. Has better application prospect in the aspects of dairy water treatment and resource utilization.

Inventors

  • WANG MIN
  • ZHANG YONGHONG
  • SHEN YANBING
  • MA AIJING
  • WU YUHAN
  • SU ZHENHUA

Assignees

  • 天津科技大学

Dates

Publication Date
20260508
Application Date
20260409

Claims (9)

  1. 1. The Kluyveromyces marxianus (Kluyveromyces marxianus) is characterized in that the Kluyveromyces marxianus is named as ART-27, the classification name is Kluyveromyces marxianus, the preservation number is CGMCC No.39119, the preservation date is 2026, 1 month and 7 days, the preservation unit is China general microbiological culture Collection center (CGMCC for short), and the China general microbiological culture Collection center (China) is named as No.3 in the West Lax 1 of North Star in the Chart area of Beijing.
  2. 2. Use of kluyveromyces marxianus as defined in claim 1 for treating dairy water.
  3. 3. The method of claim 2, wherein the milk water comprises milk waste water, milk pipe flush water, milk tank flush water, and milk water.
  4. 4. A method for treating low concentration dairy rinse water using kluyveromyces marxianus as defined in claim 1 comprising the steps of: (1) Activating and preparing seed liquid, namely carrying out activation culture on Kluyveromyces marxianus to obtain activated seed liquid; (2) Preparing bacterial suspension, namely centrifugally collecting bacterial cells from the activated seed liquid obtained in the step (1), washing, and then re-suspending in normal saline to prepare high-activity bacterial suspension; (3) And (3) fermentation treatment, namely inoculating the high-activity bacterial suspension prepared in the step (2) into low-concentration dairy wash water for fermentation.
  5. 5. The method according to claim 4, wherein the step (1) is performed by streaking the strain, selecting single colonies, inoculating the single colonies into YPD liquid medium, and culturing the strain at 30-42 ℃ for 16-24 hours.
  6. 6. The method according to claim 4 or 5, wherein the low concentration milk rinse water in step (3) contains lactose 5 g/L and protein 5 g/L.
  7. 7. Use of kluyveromyces marxianus as defined in claim 1 for the efficient use of lactose and/or protein in low concentration dairy components.
  8. 8. The method for efficiently utilizing the micromolecular substances lactose and protein by utilizing the Kluyveromyces marxianus as claimed in claim 1 is characterized by comprising the following steps: (1) Preparing seed solution, taking out preserved yeast strain from-80 deg.C refrigerator, streaking and activating for 2 times, inoculating single colony into YPD liquid culture medium, culturing at 30-42 deg.C for 16 h, centrifuging, washing with 10XPBS for 3 times, and re-suspending with 10 xBS of the same volume as YPD liquid culture medium; (2) The biomass of the saccharomycetes is diluted to OD 600 =1, and the total inoculum size of 2% is added into the dairy waste water, and the pH is regulated to 6; (3) Fermentation is carried out at 48 h.
  9. 9. The method of claim 8, wherein the dairy wastewater comprises 5g/L lactose, 5g/L protein, sulfate, and phosphate.

Description

Kluyveromyces marxianus, method and application thereof Technical Field The invention belongs to the technical field of resource and environmental microorganism, and particularly relates to Kluyveromyces marxianus, a method and application thereof. Background In the dairy production process, the consumption of non-product water is often up to several tons or even more than ten tons per ton of end products such as sterilized milk or fermented milk, wherein the cleaning water accounts for over 80-85%. The production mode of 'consuming a large amount of water for cleaning' is indispensable in guaranteeing food safety, but also causes 'high input and high discharge' resource metabolism dilemma, obviously increases the water taking cost and sewage treatment burden of enterprises, and brings continuous environmental pollution risks. At present, most of the cleaning wastewater generated in the dairy processing process adopts a treatment method of collecting the cleaning wastewater in a concentrated way and then carrying out physical chemistry, and specific technologies comprise flocculation, membrane filtration and the like. This mode makes the components such as lactose and protein, which are originally contained in the washing water from the equipment and the pipeline, mixed and diluted by other low-concentration waste water, and the water flow formed by the washing of the equipment and the conveying system contains a large amount of biodegradable organic substances such as lactose and protein, etc., so that the water flow is very suitable for being used as a nutrition source for culturing microorganisms. Therefore, the treatment objective of the wastewater is to convert the degradation of pure pollutants into resource recovery and reuse, such as microbial proteins, water and the like. However, due to the characteristic of low concentration of organic matters in milk washing water, the existing microorganism strain has the problems of slow growth, low substrate conversion rate and the like in low-concentration milk waste water, and the large-scale application of the microorganism strain in the low-concentration milk waste water treatment is severely restricted. Therefore, the breeding of excellent strains for efficiently utilizing low-concentration dairy components is one of important subjects for solving the application of dairy water reclamation, and has important practical value and academic research value. Disclosure of Invention The invention aims to overcome the defects in the prior art and provide Kluyveromyces marxianus which can efficiently flocculate macromolecular proteins in dairy water and utilize lactose, proteins and other components. The technical scheme adopted for solving the technical problems is as follows: The Kluyveromyces marxianus (Kluyveromyces marxianus) is characterized in that the Kluyveromyces marxianus is named as ART-27, the classification name is Kluyveromyces marxianus, the preservation number is CGMCC No.39119, the preservation date is 2026, 1 month and 7 days, the preservation unit is China general microbiological culture Collection center (CGMCC for short), and the China general microbiological culture Collection center (China) is named as No.3 in the West Lax 1 of North Star in the Chart area of Beijing. Use of kluyveromyces marxianus as described above in the treatment of dairy water. Further, the dairy water comprises dairy wastewater, dairy pipeline flushing water, dairy tank flushing water, dairy water and the like. A method for treating low concentration dairy rinse water using kluyveromyces marxianus as described above comprising the steps of: (1) Activating and preparing seed liquid, namely carrying out activation culture on Kluyveromyces marxianus to obtain activated seed liquid; (2) Preparing bacterial suspension, namely centrifugally collecting bacterial cells from the activated seed liquid obtained in the step (1), washing, and then re-suspending in normal saline to prepare high-activity bacterial suspension; (3) And (3) fermentation treatment, namely inoculating the high-activity bacterial suspension prepared in the step (2) into low-concentration dairy wash water for fermentation. Further, in the step (1), single colony is selected after the strain is streaked, inoculated in YPD liquid culture medium and cultured for 16-24 hours at 30-42 ℃. Further, the low-concentration dairy wash water in the step (3) contains 5g/L lactose and 5g/L protein. The use of kluyveromyces marxianus as described above for the efficient use of lactose and/or protein in low concentration dairy components. The method for efficiently utilizing the micromolecular substances lactose and protein by utilizing the Kluyveromyces marxianus comprises the following steps: (1) Preparing seed solution, namely taking out preserved saccharomycete strain from a refrigerator with ultralow temperature of-80 ℃ and carrying out streaking activation on a flat plate for 2 times, selecting single bacterial col