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CN-121991825-A - Probiotic composition and use thereof

CN121991825ACN 121991825 ACN121991825 ACN 121991825ACN-121991825-A

Abstract

The invention belongs to the technical field of microorganisms, and provides a probiotic composition and application thereof. The probiotic composition comprises bacillus subtilis (Bacillus subtilis) and optionally lactobacillus plantarum (Lactobacillus plantarum). The bacillus subtilis and the lactobacillus plantarum have excellent Alcohol Dehydrogenase (ADH) activity, wherein when the bacillus subtilis is singly applied or is compounded with the lactobacillus plantarum for use, the ADH activity in a human body can be improved, the alcohol metabolism capability is enhanced, the sobering time is shortened, and the concentration of alcohol in blood can be effectively reduced. Meanwhile, the probiotic composition can promote the activity of antioxidant enzymes in intestinal tracts, enhance the antioxidant capacity of the intestinal tracts by scavenging free radicals, further reduce free radical tissue damage caused by ethanol intake, and also can remarkably promote the species richness and diversity of intestinal flora and construct stable and healthy intestinal microecology.

Inventors

  • GUAN PENG
  • LOU XIAOFEN

Assignees

  • 北京精至医信科技有限公司

Dates

Publication Date
20260508
Application Date
20260210

Claims (10)

  1. 1. A probiotic composition, characterized by comprising Bacillus subtilis (Bacillus subtilis) and optionally Lactobacillus plantarum (Lactobacillus plantarum), wherein, The preservation number of the bacillus subtilis in the China general microbiological culture Collection center of the China Committee for culture Collection of microorganisms is CGMCC No.34922, and the preservation date is 2025, 06 and 16; the preservation number of the lactobacillus plantarum in the China general microbiological culture Collection center of the China Committee for culture Collection of microorganisms is CGMCC No.34715, and the preservation date is 2025, 05 and 29 days.
  2. 2. The probiotic composition according to claim 1, characterized in that the ratio of the number of viable bacteria of said bacillus subtilis to the number of viable bacteria of said lactobacillus plantarum is (1-3) 1; In the probiotic composition, the total number of viable bacteria is not less than 5×10 7 CFU/g.
  3. 3. A microbial agent comprising a probiotic composition according to any one of claims 1-2.
  4. 4. A lyophilized formulation comprising the probiotic composition of any one of claims 1-2 or the microbial agent of claim 3.
  5. 5. The lyophilized formulation according to claim 4, further comprising a lyoprotectant comprising trehalose, inulin, and whey protein.
  6. 6. The lyophilized formulation according to claim 5, wherein the mass ratio of trehalose, inulin and whey protein is (1-1.5): 1 (1-1.5).
  7. 7. An anti-hangover product comprising a probiotic composition according to any one of claims 1 to 2, a microbial agent according to claim 3 or a lyophilized formulation according to any one of claims 4 to 6.
  8. 8. An anti-alcohol product according to claim 7, wherein the anti-alcohol product comprises a food or pharmaceutical product.
  9. 9. Use of the probiotic composition of any one of claims 1-2, the microbial agent of claim 3, the lyophilized formulation of any one of claims 4-6, the anti-hangover product of any one of claims 7-8, comprising at least one of the following: for elevating ADH activity; For reducing the concentration of alcohol in blood; for enhancing the activity of antioxidant enzymes; For improving the species number and uniformity of the intestinal flora; use in the manufacture of a medicament for reducing alcohol concentration in blood, increasing ADH activity, increasing antioxidant enzyme activity or increasing the number and uniformity of species of the intestinal flora.
  10. 10. A method of reducing the concentration of intracellular ethanol or increasing the activity of intracellular ADH or increasing the activity of intracellular antioxidant enzymes in vitro comprising: Co-culturing at least one of the probiotic composition of any one of claims 1-2, the microbial agent of claim 3, the lyophilized formulation of any one of claims 4-6, the anti-hangover product of any one of claims 7-8 with the cells.

Description

Probiotic composition and use thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to a probiotic composition and application thereof. Background Alcohol Dehydrogenase (ADH) is a major component of the primary metabolism of the human body, and is found mainly in the liver and gastrointestinal tract. Typically, ethanol rapidly enters the stomach and intestines, diffuses through the intestinal mucosa into the systemic circulation, and is delivered to the liver via the portal vein. Alcohol dehydrogenase and aldehyde dehydrogenase (ALDH) of the liver are responsible for oxidizing alcohol, converting it to oncogenic acetaldehyde, and ultimately to non-toxic acetic acid. However, excessive alcohol consumption can lead to acetaldehyde accumulation in the liver, disrupt liver metabolism, and cause liver lesions. The essence of the anti-alcohol is that alcohol dehydrogenase oxidizes alcohol to acetaldehyde, then the acetaldehyde dehydrogenase changes into acetic acid, and the acetic acid enters the blood circulation and is metabolized into carbon dioxide, water and energy. Therefore, the alcohol dispelling mechanism is to activate ADH production in the body, increase ADH activity, accelerate the oxidative decomposition of ethanol in the body, reduce the concentration of ethanol in the body, shorten the drunk time and relieve the inadaptation of people caused by drunk. The existing anti-alcoholic medicines comprise metadoxine, naloxone and the like, and have a relatively large number of adverse reactions although having an anti-alcoholic effect. For example, metadoxine is an activator of acetaldehyde dehydrogenase, which antagonizes the decline of alcohol dehydrogenase activity caused by acute and chronic alcoholism and accelerates the excretion of alcohol and its metabolites acetaldehyde and ketone bodies through urine, but metadoxine may cause peripheral nerve diseases. Naloxone is an antidote for opioid overdose and can also treat alcoholism, but can cause side effects such as mania. Recently, it has been found that the use of probiotics expressing alcohol dehydrogenase for alleviating alcohol is safer, more effective and more body friendly than intravenous administration. Therefore, there is a need to develop strains with high alcohol dehydrogenases. Disclosure of Invention The present invention aims to solve at least one of the technical problems existing in the prior art to at least some extent. Therefore, the invention provides a probiotic composition, which comprises bacillus subtilis and optionally lactobacillus plantarum, wherein the bacillus subtilis and the lactobacillus plantarum are both derived from feces of subjects with good wine, have good ADH activity, can effectively reduce the alcohol concentration in blood, and improve the activity of antioxidant enzymes (such as superoxide dismutase and catalase), so that the damage of free radicals to tissues after ethanol intake is reduced. Thus, in a first aspect of the invention, the invention proposes a probiotic composition. According to the embodiment of the invention, the probiotic composition comprises bacillus subtilis (Bacillus subtilis) and optionally lactobacillus plantarum (Lactobacillus plantarum), wherein the collection number of the bacillus subtilis in the China general microbiological culture collection center is CGMCC No.34922, the collection date is 2025 and month 16, and the collection number of the lactobacillus plantarum in the China general microbiological culture collection center is CGMCC No.34715 and the collection date is 2025 and month 29. The inventor finds through a large number of experiments that the bacillus subtilis and the lactobacillus plantarum have excellent Alcohol Dehydrogenase (ADH) activity, wherein when the bacillus subtilis is singly applied or is compounded with the lactobacillus plantarum for use, the ADH activity in a body can be improved, the alcohol metabolism capacity can be enhanced, the sobering-up time can be shortened, and the concentration of alcohol in blood can be effectively reduced. Meanwhile, the probiotic composition can regulate the balance of intestinal flora, promote the activity of antioxidant enzymes in the intestinal tract, strengthen the antioxidant capacity of the intestinal tract by scavenging free radicals, further reduce the free radical tissue damage caused by ethanol intake, and also can obviously promote the species richness and diversity of the intestinal flora and construct stable and healthy intestinal microecology. In a second aspect of the invention, the invention provides a microbial agent. According to an embodiment of the invention, the microbial agent comprises the probiotic composition of the first aspect. Therefore, the bacillus subtilis and the lactobacillus plantarum have excellent Alcohol Dehydrogenase (ADH) activity, wherein when the bacillus subtilis is singly applied or is compounded with the lactobacillus pla