CN-121991826-A - Pseudomonas exo DHS-HJ-IAA-2 resistant to saline and alkaline and promoting growth and application thereof

Abstract

The invention discloses a Pseudomonas exocarpium DHS-HJ-IAA-2 resistant to saline and alkaline and promoting growth and application thereof, and belongs to the technical field of microorganisms. The invention screens out a strain of salt-resistant, alkali-resistant and drought-resistant pseudomonas exo DHS-HJ-IAA-2 which is preserved in China general microbiological culture Collection center with the preservation number of CGMCC No.36164. Experimental results show that the pseudomonas exocarpium DHS-HJ-IAA-2 has the functions of IAA production, nitrogen fixation, potassium decomposition, cellulose and lignin degradation, plant pathogenic bacteria inhibition and the like, can remarkably improve the bacterial abundance of saline-alkali soil, improve the soil fertility, and promote plant growth in saline-alkali environment. The invention provides new strain resources and technical means for improving the saline-alkali soil and planting crops on the saline-alkali soil, and realizes the improvement of the saline-alkali soil from two aspects of soil-crop-microorganism comprehensive action and microorganism-plant synergistic action.

Inventors

• ZHANG XIAOQING
• ZHANG AIJUN
• SI XIAOGUANG
• CAO JUNRUI
• REN HUAFENG

Assignees

• 自然资源部天津海水淡化与综合利用研究所

Dates

Publication Date

20260508

Application Date

20260212

Priority Date

20260122

Claims (10)

1. 1. The Pseudomonas exo (Ectopseudomonas sp.) DHS-HJ-IAA-2 is characterized in that the Pseudomonas exo DHS-HJ-IAA-2 is preserved in China general microbiological culture Collection center (CGMCC) No.36164 at a preservation address of North Star West Song No. 1, no.3 of the Korean region North Star in Beijing, 10 months 13 of 2025.
2. 2. Use of pseudomonas exocarpium DHS-HJ-IAA-2 according to claim 1 for the preparation of a growth promoting agent for promoting plant growth.
3. 3. A growth-promoting microbial agent comprising the Pseudomonas exocarpium DHS-HJ-IAA-2 according to claim 1 as an active ingredient.
4. 4. A growth promoting agent according to claim 3, wherein the viable bacteria concentration of the pseudomonas exocarpium DHS-HJ-IAA-2 in the growth promoting agent is not less than 2 x 10 8 CFU/mL.
5. 5. Use of a pseudomonas exo DHS-HJ-IAA-2 according to claim 1 or a growth promoting agent according to claim 3 or 4 in any of the following: (1) Nitrogen fixation; (2) Producing IAA; (3) Potassium decomposition; (4) Decomposing cellulose: (5) Decomposing lignin; (6) Inhibit pathogenic fungi.
6. 6. Use of a pseudomonas exocarpium DHS-HJ-IAA-2 according to claim 1 or a growth promoting agent according to claim 3 or 4 for promoting plant growth and/or improving plant resistance to salt and alkaline stress.
7. 7. A method of promoting plant growth and/or improving the saline-alkali stress tolerance of a plant, comprising the step of applying the pseudomonas exocarpium DHS-HJ-IAA-2 according to claim 1 or the growth promoting microbial agent according to claim 3 or 4 to a plant growth environment.
8. 8. The method of claim 7, wherein the plant growing environment is a salt-containing and/or alkaline environment.
9. 9. Use of the pseudomonas exocarpium DHS-HJ-IAA-2 according to claim 1 or the growth promoting agent according to claim 3 or 4 for improving saline-alkali soil.
10. 10. A method of improving saline-alkali soil, comprising the step of applying the pseudomonas exo DHS-HJ-IAA-2 of claim 1 or the growth promoting agent of claim 3 or 4 to the saline-alkali soil.

Description

Pseudomonas exo DHS-HJ-IAA-2 resistant to saline and alkaline and promoting growth and application thereof Technical Field The invention relates to the technical field of microorganisms, in particular to pseudomonas exocarpium DHS-HJ-IAA-2 resistant to saline and alkaline and promoting growth and application thereof. Background Soil salinization is a ubiquitous ecological problem and has become a focus and a hotspot of soil ecological restoration. Soil salinization not only directly damages crop growth and reduces land productivity, but also can cause environmental problems such as water and soil loss, land desertification and the like, and limits the benign development of economy. The traditional physical and chemical restoration method is applied to the improvement of the saline-alkali soil to a certain extent, but has the defects of water resource waste, large investment, complex engineering and the like, and is easy to bring about secondary environmental pollution in other forms. Compared with the method, the method has the advantages of improving the soil microorganism system by utilizing beneficial bacteria, reducing the salt alkalinity of the soil, improving the nutrient utilization rate, promoting the synthesis of soil organic matters, increasing the available nutrients of the soil, regulating the plant growth, enhancing the stress resistance of the plant, along with economy, high efficiency, no residue in crops, energy and fresh water conservation, wide application range and the like, can also prevent environmental pollution and soil deterioration, and has great application potential in low-fertility saline-alkali soil. However, the microbial remediation technology still faces two major bottlenecks that ① high-saline-alkali adaptive strains are few, and the microbial agent in ② high-saline-alkali environment is low in activity and difficult to colonize, and the synergistic effect is difficult to play. The research angle in the current field is often focused on the growth promotion effect of microorganisms on plants, and the consideration of the comprehensive effect of soil-plants-microorganisms and the analysis of the soil improvement effect under the synergistic effect of the microorganisms and the plants are lacked. The rhizosphere growth-promoting bacteria are used as important constituent factors of soil and are closely related to plant growth conditions and soil environments in salinized soil. Some plant growth promoting bacteria not only can dissolve nutrient ions in soil, secrete plant growth factors, promote plant growth and improve the diversity of soil microorganisms, but also can improve the salinity characteristics of the soil and improve the salt tolerance of plants. Therefore, the functions of saline-alkali resistance, growth promotion and the like of the microorganism are utilized to improve the soil environment for plant growth and promote the growth and development of plants, and the method is a safe, efficient and environment-friendly salinized soil restoration measure. There is a need to separate and identify rhizosphere growth promoting bacteria with saline-alkali tolerance and various growth promoting characteristics, so as to provide important functional strain resources and technical foundation for preparing microbial agents for improving saline-alkali soil. Disclosure of Invention The invention aims to provide a Pseudomonas exo DHS-HJ-IAA-2 strain resistant to saline-alkali and promoting growth and application thereof, so as to solve the problems of the prior art, provide new strain resources and technical means for saline-alkali soil improvement and crop planting on the saline-alkali soil, and realize the improvement of the saline-alkali soil from two aspects of soil-crop-microorganism comprehensive action and microorganism-plant synergistic action. In order to achieve the above object, the present invention provides the following solutions: the invention provides pseudomonas exocarpium (Ectoppseudomonasp.) DHS-HJ-IAA-2, wherein the pseudomonas exocarpium DHS-HJ-IAA-2 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.36164 and the preservation address of North Star Xway No.1, 3 in the Chaoyang area of Beijing, on the day of 10 months 13 of 2025. The invention also provides application of the pseudomonas exocarpium DHS-HJ-IAA-2 in preparing a growth promoting microbial agent for promoting plant growth. The invention also provides a growth promoting microbial agent which takes the pseudomonas exocarpium DHS-HJ-IAA-2 as an active ingredient. Further, in the growth promoting microbial agent, the living microbial concentration of the pseudomonas exocarpium DHS-HJ-IAA-2 is not less than 2X 10 8 CFU/mL. The invention also provides application of the pseudomonas exocarpium DHS-HJ-IAA-2 or the growth promoting agent in any one of the following: (1) Nitrogen fixation; (2) Producing IAA; (3) Potassium decomposition