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CN-121991829-A - Microbial preparation for preventing and controlling plant root knot nematode as well as preparation method and application thereof

CN121991829ACN 121991829 ACN121991829 ACN 121991829ACN-121991829-A

Abstract

The application discloses a microbial preparation for preventing and controlling plant root knot nematodes, and a preparation method and application thereof, and belongs to the technical field of microbial preparations. The microbial preparation for preventing and controlling plant root knot nematodes is of a double-layer coating structure, and comprises an inner layer and an outer layer, wherein the inner layer comprises rhodotorula fermentation liquor, paecilomyces lilacinus fermentation liquor, bacillus subtilis fermentation liquor, bacillus thuringiensis fermentation liquor, beauveria bassiana fermentation liquor and an inner layer carrier material, and the outer layer comprises Streptomyces pachinensis fermentation liquor and an outer layer carrier material.

Inventors

  • LIU TINGTING
  • FANG HAO
  • FANG YONGSHENG

Assignees

  • 潍坊绿威特生物工程有限公司

Dates

Publication Date
20260508
Application Date
20260407

Claims (10)

  1. 1. A microbial preparation for preventing and controlling plant root-knot nematodes is characterized by comprising a double-layer coating structure and an inner layer and an outer layer, wherein the inner layer comprises rhodozyma fermentation liquor, paecilomyces lilacinus fermentation liquor, bacillus subtilis fermentation liquor, bacillus thuringiensis fermentation liquor, beauveria bassiana fermentation liquor and an inner layer carrier material, the outer layer comprises streptomyces pachyrhizium fermentation liquor and the outer layer carrier material, the volume mass ratio of the sum of the inner layer fermentation liquor to the inner layer carrier material is 100ml (5-8) g, and the volume mass ratio of the outer layer streptomyces pachyrhizium fermentation liquor to the outer layer carrier material is 100ml (1.5-3.5) g.
  2. 2. The microbial preparation for preventing and controlling plant root-knot nematodes, according to claim 1, wherein the volume ratio of the rhodozyma fermentation liquor, the paecilomyces lilacinus fermentation liquor, the bacillus subtilis fermentation liquor, the bacillus thuringiensis fermentation liquor, the beauveria bassiana fermentation liquor and the streptomyces pachyrhizi fermentation liquor is (2-3): 4-5): 1.5-2.5): 1:1:1.
  3. 3. The microbial preparation for preventing and controlling plant root knot nematodes according to claim 1, wherein the inner carrier material comprises 1.5-1.8 parts of sodium alginate, 2.0-2.5 parts of chitin powder, 0.3-0.5 parts of curcuma zedoary extract, 0.1-0.2 parts of radix sophorae flavescentis extract and 2.0-2.2 parts of modified starch; the outer layer carrier material comprises 1.0-1.3 parts of chitosan, 0.5-0.6 part of humic acid oligosaccharide, 0.2-0.5 part of cinnamon extract, 0.15-0.25 part of chinaberry bark extract and 0.2-0.3 part of rhizosphere adsorbent.
  4. 4. The microbial preparation for preventing and controlling plant root knot nematodes of claim 3, wherein the inner carrier material further comprises dolomite powder 0.8-1 part by weight; the outer layer carrier material further comprises 0.3-0.4 part of chitinase inducer.
  5. 5. The microbial preparation for controlling plant root knot nematodes as defined in claim 4, wherein said inner carrier material further comprises 0.4 parts by weight of polylysine.
  6. 6. A microbial preparation for controlling plant root knot nematodes according to claim 4, wherein the modified starch is corn starch pretreated with an alpha-amylase at 60-65deg.C for 30-40 min, and/or The rhizosphere adsorbent comprises sodium modified bentonite and potassium humate, wherein the mass ratio of the sodium modified bentonite to the potassium humate is 1 (0.9-1.1), and/or The chitinase inducer comprises chitinolycins.
  7. 7. The microbial preparation for preventing and controlling plant root knot nematode according to claim 1, wherein in the microbial preparation, the viable count of rhodotorula fermentation broth is not less than 5.0X10 8 CFU/mL, the viable count of paecilomyces lilacinus fermentation broth is not less than 6.0X10 8 CFU/mL, the conidium concentration is not less than 5.0X10 8 /mL, the viable count of bacillus subtilis fermentation broth is not less than 8.0X10 8 CFU/mL, the viable count of bacillus thuringiensis fermentation broth is not less than 5.0X10 8 CFU/mL, the viable count of beauveria bassiana fermentation broth is not less than 5.0X10 8 CFU/mL, the conidium concentration is not less than 4.0X10 8 /mL, and the viable count of Streptomyces pachinensis fermentation broth is not less than 5.0X10 8 CFU/mL.
  8. 8. The method for preparing the microbial preparation for preventing and treating plant root knot nematodes according to any one of claims 1 to 7, which is characterized by comprising the following steps: (1) Respectively preparing rhodozyma fermentation liquor, paecilomyces lilacinus fermentation liquor, bacillus subtilis fermentation liquor, bacillus thuringiensis fermentation liquor, beauveria bassiana fermentation liquor and streptomyces compactus fermentation liquor according to the volume ratio, and mixing the rhodozyma fermentation liquor, paecilomyces lilacinus fermentation liquor, bacillus subtilis fermentation liquor, bacillus thuringiensis fermentation liquor and beauveria bassiana fermentation liquor to obtain an inner layer mixed fermentation liquor; (2) Weighing inner layer carrier materials according to a proportion, mixing and dissolving, sterilizing and cooling to room temperature to obtain inner layer carrier material liquid; (3) Uniformly dripping the suspension into calcium chloride curing liquid for constant temperature curing, flushing and filtering to obtain inner microspheres; (4) And mixing and dissolving the outer layer carrier material, sterilizing and cooling to room temperature, mixing with the Streptomyces cescens fermentation broth, coating the surface of the inner layer microsphere by adopting a roll coating method, solidifying at constant temperature, drying and screening to obtain the microbial preparation.
  9. 9. The preparation method of the microbial preparation for preventing and controlling plant root knot nematodes, which is characterized by comprising the steps of (3) enabling the mass concentration of calcium chloride curing liquid to be 1.8% -2.0%, the curing temperature to be 28% -30 ℃ and the curing time to be 10% -12 h, and (4) enabling the curing temperature to be 25% -27 ℃ and the curing time to be 8% -9 h, wherein the drying temperature to be 35% -40 ℃ and the water content of the preparation after drying to be less than or equal to 8%.
  10. 10. The use of a microbial preparation for controlling plant root knot nematodes according to any one of claims 1 to 7 or a microbial preparation prepared by a preparation method according to any one of claims 8 to 9 for controlling root knot nematodes.

Description

Microbial preparation for preventing and controlling plant root knot nematode as well as preparation method and application thereof Technical Field The application relates to a microbial preparation for preventing and controlling plant root knot nematodes, and a preparation method and application thereof, and belongs to the technical field of microbial preparations. Background Root knot nematode disease is one of the most damaging soil-borne diseases in global agricultural production, and is widely harmful to more than 2000 plants such as vegetables, fruit trees, cash crops and the like. The pathogenic nematodes invade plant root systems to form giant cells, so that root tissues are deformed and expanded to form root knots, moisture and nutrient absorption are seriously hindered, and plant wilting, growth arrest and yield loss can reach 30% -80%, even are stopped. At present, the prevention and treatment means of the root-knot nematode mainly comprise chemical prevention and treatment, physical prevention and treatment and biological prevention and treatment. The chemical control method has the advantages that the chemical control method is quick in effect, but the single chemical agent is easy to cause drug resistance of nematodes after long-term use, soil, water and crops are polluted by chemical agent residues, the ecological environment and the quality safety of agricultural products are threatened, the green agricultural development requirements are not met, the physical control (such as soil fumigation and rotation) is complex in operation and high in cost, and is limited by land resources and planting modes, and the chemical control method is difficult to popularize and apply in large-scale production. Therefore, the biological control technology using the microbial preparation as the core has the advantages of environmental friendliness, no residue, sustainable pest control and the like, and becomes the main development direction of root-knot nematode control. At present, paecilomyces lilacinus has strong parasitic capability, is a natural enemy fungus for effectively preventing and controlling root-knot nematodes at present, but single strain is difficult to effectively kill the root-knot nematodes, has relatively low activity and does not exert ecological effect of microorganisms in soil rhizosphere. Chinese patent CN 114424776B discloses a compound microbial agent for controlling southern root-knot nematode and a preparation method thereof, and in particular discloses a compound bacterial powder prepared by compounding four strains, wherein the control rate of the compound bacterial powder on southern root-knot nematode is more than 80%, and the control effect of the compound bacterial powder on southern root-knot nematode is obviously better than that of a single bacterial powder. The method has the advantages that the optimal growth pH of the four strains in the patent is 6.5-7.5, the soil in the retransmission zone of the root-knot nematodes is an acidic environment with the pH of 5.5-6.8, the activity of the strains is attenuated under the acidic condition, the efficiency of killing the root-knot nematodes is greatly reduced, in addition, the strains are simply mixed to obtain the composite strain, the strain is easy to run off, the shelf life is short, the prevention and treatment effect is reduced along with the time, the prevention and treatment effect of the root-knot nematodes is further influenced, and the long-acting pest control requirement cannot be met. Therefore, how to provide a microbial preparation which is suitable for the acid soil environment of the root-knot nematode re-sending area and can control harmful effects for a long time becomes an urgent need for root-knot nematode control. Disclosure of Invention In order to solve the problems, the microbial preparation for preventing and controlling plant root-knot nematodes, the preparation method and the application thereof are provided, and the microbial preparation is compounded by adopting rhodotorula, paecilomyces lilacinus, bacillus subtilis, bacillus thuringiensis fermentation liquor, beauveria bassiana fermentation liquor and streptomyces compactus, and the synergistic effect of six strains is realized, so that the functional complementation is realized, the double-layer coating structure is adapted to acid soil, the initial activity of the strain is ensured, the survival efficiency of the strain is improved, the shelf life and the function playing period are prolonged, and the long-acting pest control is realized. According to one aspect of the application, a microbial preparation for preventing and controlling plant root knot nematodes is provided, the microbial preparation is of a double-layer coating structure and comprises an inner layer and an outer layer, the inner layer comprises a rhodotorula rhodozyma fermentation liquor, a paecilomyces lilacinus fermentation liquor, a bacillus subtilis fermentation liquor, a b