CN-121991830-A - Extracellular multimeric Sphingomonas and application thereof

Abstract

The invention relates to the technical field of oilfield biochemistry, in particular to extracellular polymeric substance Sphingomonas (Sphingomonas sanxanigenens) and application thereof. The preservation number of the extracellular polymeric sphingomonas strain is CGMCC No.30927. The biological flocculant prepared by the extracellular polymeric sphingomonas can flocculate crude oil in crude oil-containing sewage, can recover crude oil from crude oil-containing sewage, has high crude oil recovery efficiency, low solid content of reinjection water obtained by treatment, and less pollution to stratum, and has the advantages of high efficiency, simplicity and convenience and low cost.

Inventors

• LI ZHUANGZHUANG
• WU WEILIN
• YANG FAN
• ZHU JIE
• GONG JIAYU
• SHI YUQING

Assignees

• 中国石油化工股份有限公司
• 中国石油化工股份有限公司江苏油田分公司

Dates

Publication Date

20260508

Application Date

20241030

Claims (10)

1. 1. An extracellular multimeric Sphingomonas strain JD24001WB (Sphingomonas sanxanigenens) is characterized in that the extracellular multimeric Sphingomonas strain has a preservation number of CGMCC No. 30927.
2. 2. Use of the extracellular polymeric substance sphingomonas according to claim 1 for the preparation of a flocculant.
3. 3. A method for preparing a bioflocculant, which comprises inoculating the extracellular polymeric substance Sphingomonas according to claim 1 into a liquid medium for culturing.
4. 4. A process according to claim 3, wherein the medium contains 30-50g/L glucose, 0.1-2.0g/L yeast extract, 0.5-2.0g/L sodium nitrate, 0.5-1.5g/L dipotassium hydrogen phosphate, 0.2-1g/L potassium dihydrogen phosphate, 0.1-0.6g/L magnesium sulfate, 0.1-0.5g/L calcium carbonate, 0.1-1g/L potassium chloride, pH 7.0-8.0, and/or The culture conditions comprise a temperature of 28-35 ℃ and a time of 24-72h.
5. 5. The bioflocculant prepared by the preparation method of claim 3 or 4.
6. 6. Use of the extracellular polymeric substance Sphingomonas and/or its fermentation broth according to claim 1, the bioflocculant according to claim 5 for flocculating oily sewage.
7. 7. A method for flocculating wastewater containing crude oil, which comprises mixing a fermentation broth of a strain containing the extracellular polymeric substance Sphingomonas according to claim 1 or the bioflocculant according to claim 5 with the wastewater containing crude oil.
8. 8. The method of claim 7, wherein, Mixing at a temperature of 20-60℃, and/or The pH of the mixed solution is 3-10.
9. 9. The method according to claim 7 or 8, wherein, The oily sewage comprises water, crude oil and solid matters, preferably, the crude oil content is 50-200mg/L, and/or the solid matters content is 0-100mg/L; More preferably, the crude oil is light crude oil, wherein the alkane is 50-90wt% and the arene is 10-30wt%.
10. 10. The method according to any one of claims 7-9, wherein, The method also comprises the steps of carrying out solid-liquid separation on the flocculated system, and extracting the solid to obtain recovered crude oil.

Description

Extracellular multimeric Sphingomonas and application thereof Technical Field The invention relates to the technical field of oilfield biochemistry, in particular to extracellular polymeric substance Sphingomonas and application thereof. Background Sphingomonas was isolated and identified by scientists in 1990 until 2001, and was classified into 4 genera, sphingomonas (Sphingomonas), sphingomonas (Sphingobium), novel Sphingomonas (Novosphingobium), coleoptera (Sphigopyxis) in taxonomy due to physical characteristics, phylogenetic characteristics, and the like. Sphingomonas (Sphingomonas) is a gram-negative bacterium, rod-shaped, typically aerobic, chemical heterotrophic, with a unique respiratory metabolic pathway, coenzyme Q10, and the cell membrane composition mainly contains glycosphingolipids rather than lipopolysaccharides, usually secreting pigments to make the colony yellow. Sphingomonas has been receiving more and more attention in the field of oil and gas microbiology, wherein Sphingomonas also has biosynthesis capability, and secretes extracellular polymers such as Gellan Gum (Gellan Gum), welan Gum (Welan Gum), rhamnose Gum (Rhamnose Gum) and the like, and is widely applied to the petroleum industry, and mainly depends on chemical and physical properties of polysaccharide such as viscosity, gel property and the like. For example, CN107058187a discloses a sphingomonas paucimobilis strain and a method for using the same, provides a new sphingomonas paucimobilis strain FJAT-10625 (Sphingomonas paucimobilis), and uses the strain to prepare and obtain extracellular polysaccharide with higher yield, thereby increasing the sources of extracellular polysaccharide production strains. CN103421718B discloses a sphingomonas paucimobilis strain and a method for applying the same, and the sphingomonas paucimobilis strain is endophyte obtained by separating and screening from wild microporous grass seeds in Qinghai-Tibet plateau, can be used for the industrial production of gellan gum, and provides a new way for the industrial production of gellan gum by endophyte. CN115838646a discloses a sphingomonas paucimobilis SLDT21 and a method for applying the same, and provides application of the sphingomonas paucimobilis SLDT21 in petroleum extraction, microbial oil extraction and as a biological polysaccharide oil displacement agent. In addition, exopolysaccharides secreted by Sphingomonas can be used as flocculants. For example, CN113151050B discloses the use of sphingomonas (Sphingomonas sanxanigenens) HL1 strain in the preparation of microbial flocculants. CN115636515A discloses an environment-friendly sewage treatment flocculant for microbial oil recovery, which is characterized in that polysaccharide is produced by Sphingomonas, such as arabinose, glucosamine hydrochloride, galactose, glucose, mannans, galacturonic acid and guluronic acid, and the novel polysaccharide has good flocculation effect, but composite polymeric ferric chloride is needed to meet the flocculation effect. However, aiming at the treatment of crude oil-containing sewage to be reinjected and the recovery of crude oil in the oil-containing sewage, technologies such as filtration, air floatation treatment, membrane separation and the like are adopted at present, for example, an elm oil field adopts an aeration, cavitation air floatation, sand filtration and PVC hollow fiber ultrafiltration membrane process, a Changqing oil field adopts a gravity separation sedimentation degreasing, walnut shell coarse filtration and fiber ball fine filtration process, a heavy oil wastewater advanced treatment system taking ion exchange as a core is adopted in an Liaone oil field, an ozone-biochar technology is adopted in the Jinzhou oil field, an air floatation flocculation and biological contact oxidation method are adopted in an island oil field, and the like, but the oil-containing wastewater components generated in the petroleum exploitation and processing process are complex, and certain limitations exist in different processes in actual operation, such as the water quality of reinjection is not up to standard, the recovery rate of crude oil is low, the increasing difficulty in downstream treatment, the shock resistance is not strong, the filter material is frequently replaced, the application environment of membrane components is harsh, the operation cost is high, and the like. Disclosure of Invention In order to solve the problems of low recovery efficiency of reinjection water crude oil, high solid content and pollution to stratum existing in the crude oil-containing sewage treatment in the prior art, the invention provides an extracellular polymeric substance Sphingomonas strain and application thereof, the biological flocculant prepared by the extracellular polymeric sphingomonas can flocculate crude oil in crude oil-containing sewage, can recover crude oil from crude oil-containing sewage, has high crude oil recovery efficiency, and has low solid co