CN-121991835-A - Coxsackie strain, microbial agent and application thereof

Abstract

The invention belongs to the technical field of agricultural microorganisms, and discloses a strain of Coxsackie, named as Coxsackie (Kosakonia sacchari) P1-4, which is preserved in China center for type culture Collection (China center for type culture Collection) at 9 months and 20 days, wherein the preservation address is the university of Wuhan in China, and the preservation number is CCTCC NO: M20231759. The invention also discloses a microbial agent containing the strain and application thereof in promoting rice growth and/or improving soil health and application in reducing soil cadmium content and/or reducing rice cadmium content. The strain P1-4 of the kosakazakii and the microbial inoculum thereof provided by the invention can synchronously and obviously improve the key nutrient indexes of soil, can obviously improve the contents of nutrients such as NH 4 -N、NO 3 -N, organic phosphorus, organic matters and the like in the soil, and comprehensively improve the fertility and the bioactivity of the soil.

Inventors

• LI JUAN
• Tian Yunhe
• HU RUIWEN
• Tian meijie
• ZHENG ZHONGYI

Assignees

• 湖南农业大学

Dates

Publication Date

20260508

Application Date

20260107

Claims (10)

1. 1. A strain of the Coxsackie is characterized in that the Coxsackie is named as Coxsackie (Kosakonia sacchari) P1-4 and is preserved in China center for type culture Collection (China center for type culture Collection) at 9 months and 20 days, wherein the preservation address is the university of Wuhan, china, and the preservation number is CCTCC NO: M20231759.
2. 2. A microbial agent comprising the coxsackie bacterium (Kosakonia sacchari) P1-4 of claim 1 or comprising a fermentation broth comprising the coxsackie bacterium (Kosakonia sacchari) P1-4 of claim 1.
3. 3. Use of the microorganism bacterium agent of the kex (Kosakonia sacchari) P1-4 according to claim 1 or of the microorganism bacterium agent of claim 2 for promoting rice growth and/or improving soil health.
4. 4. The use according to claim 3, wherein said promotion of rice growth comprises an increase in biomass, root length, plant height and/or yield of rice roots, stems, leaves, ears.
5. 5. The use of claim 3, wherein improving soil health comprises increasing one or more of NH 4 -N、NO 3 -N, organic phosphorus, organic matter, pH in the soil.
6. 6. The use according to claim 3, wherein said coxsackie (Kosakonia sacchari) P1-4 or said microbial agent is irrigated into the soil.
7. 7. The use according to claim 6, wherein the microbial agent is used in an amount of 5-15L/m 2 and the OD600 value of the microbial agent is 0.8-1.1.
8. 8. Use of a microorganism bacterium agent according to claim 1 of the genus saxophone (Kosakonia sacchari) P1-4 or according to claim 2 for reducing cadmium content in soil and/or reducing cadmium content in rice.
9. 9. The use according to claim 8, wherein said kosakazakii (Kosakonia sacchari) P1-4 or said microbial agent is administered during the tillering stage of rice.
10. 10. The use according to claim 9, wherein the microbial agent is applied in an amount of 5-15L/m 2 and the OD600 value of the microbial agent is 0.8-1.1.

Description

Coxsackie strain, microbial agent and application thereof Technical Field The invention belongs to the technical field of agricultural microorganisms, and particularly relates to a coxsackie strain, a microbial agent and application thereof. Background The sustainable development of modern agriculture faces two key challenges, namely soil degradation and environmental pollution caused by long-term excessive use of chemical fertilizers, and threat of heavy metal cadmium (Cd) pollution of soil to crop safety and ecological system health. The excessive reliance on chemical fertilizers has raised a series of environmental problems. The long-term massive application causes soil hardening, acidification and salinization, damages the soil structure and the microbial activity, has low utilization rate of nitrogen, phosphorus and other nutrients, not only causes resource waste, but also aggravates eutrophication pollution after unabsorbed nutrients are leached into water. In addition, the fertilizer has high production energy consumption, and the green transformation of agriculture is severely restricted due to the emission of greenhouse gases and the accumulation of soil heavy metals in the application process. The development of microbial agents using beneficial microorganisms is an important approach to reduce fertilizer dependence. The existing microbial fertilizer can improve nutrient utilization rate, promote crop growth, and has the advantages of environmental protection, soil ecology improvement and the like through the actions of nitrogen fixation, phosphorus dissolution, growth hormone secretion and the like. However, the product has obvious limitations in practical application, that most strains have relatively single functions, mainly promote growth or nutrient activation, have insufficient stability and large effect fluctuation in different field environments, have weak restoration capability on composite stress such as soil heavy metal pollution and the like, and limit the comprehensive application effect and popularization value. On the other hand, the cadmium pollution of the soil is increasingly serious, threat to agricultural product safety and human health. The existing repair technology has the defects that the physical method (soil-removing method, soil-changing method, cleaning method, deep ploughing soil-turning method, electric repair technology and the like) needs a great deal of manpower, material resources and financial resources, the implementation difficulty is high, and the repaired soil is easy to cause secondary pollution, so that the method is difficult to popularize and apply in a large area. Chemical passivation (such as lime application) can reduce cadmium activity, but has unstable effect and is difficult to continuously control the cadmium content of crops below a safety threshold, and microbial remediation is environment-friendly, but the existing microbial inoculum has limited adsorption and fixation efficiency on cadmium, has uncertain effect in soil with different pollution degrees, and especially lacks a multifunctional bacterial strain with high-efficiency growth promotion and cadmium passivation stabilization capabilities. Therefore, the current agricultural practice is in need of a microorganism resource integrating multiple functions, which not only can efficiently activate soil nutrients, promote crop growth and increase yield, but also can synchronously cope with heavy metal pollution, and achieves the dual purposes of yield increase and safety by stably reducing the effective cadmium content of soil and preventing cadmium from migrating to crops. The development of the strain with obvious multifunctional synergistic effect and strong environmental adaptability and the application technology thereof has important significance for promoting the innovation of green input products and guaranteeing the safe production of grains. Disclosure of Invention The invention aims to solve the technical problems and overcome the defects in the background art, and provides a coxsackie strain, a microbial agent and application thereof. In order to solve the technical problems, the technical scheme provided by the invention is as follows: A strain of Coxsackie, named as Coxsackie (Kosakonia sacchari) P1-4, is preserved in China center for type culture Collection (China, university of Wuhan, china, with a preservation number of CCTCC NO: M20231759 at 9 and 20 of 2023. Based on one general inventive concept, the invention also provides a microbial agent comprising the above-mentioned Coxsackie bacteria (Kosakonia sacchari) P1-4 or comprising a fermentation broth comprising the above-mentioned Coxsackie bacteria (Kosakonia sacchari) P1-4. Based on a general inventive concept, the invention also provides an application of the Kesaxophone bacteria (Kosakonia sacchari) P1-4 or the microbial agent in promoting rice growth and/or improving soil health. Preferably, for the above-mentioned appl