CN-121991836-A - Bacillus composts A1 and application thereof in preparation of preparation for improving rice sheath blight resistance

CN121991836ACN 121991836 ACN121991836 ACN 121991836ACN-121991836-A

Abstract

The invention discloses application of bacillus composts A1 in preparation of a preparation for improving rice sheath blight resistance, relates to the technical field of biological control of microbial pesticides and plant diseases, and discloses bacillus composts (Bacillus Stercoris) A1 obtained by separation, wherein the preservation number of the bacillus composts A1 is CGMCC No.35058. The biocontrol preparation prepared by adopting the bacillus composts A1 obtained by separation has good inhibition effect on the sheath blight of rice. Because the plant-growing agent belongs to biological agents, a series of problems caused by the use of chemical pesticides are completely avoided, pollution-free production of rice is facilitated, the use amount of other chemical pesticides can be omitted or reduced, the planting cost can be reduced, and economic development of rice is facilitated.

Inventors

PENG WEIYE
KANG HOUXIANG
ZHANG DEYONG
SU PIN
Peng Qianze
GAO LIMING

Assignees

岳麓山实验室

Dates

Publication Date: 20260508
Application Date: 20260115

Claims (10)

1. A bacillus composts (Bacillus Stercoris) A1 for resisting rice sheath blight is characterized in that the preservation number is CGMCC No.35058.
2. The use of bacillus composts A1 as claimed in claim 1 for combating rice sheath blight.
3. A biocontrol agent against rice sheath blight, comprising an active ingredient and an auxiliary ingredient, wherein the active ingredient comprises bacillus composts A1 or a fermentation broth thereof according to claim 1.
4. The biocontrol preparation of claim 3, wherein the auxiliary components comprise a carrier, a dispersing auxiliary agent, a protective agent and a synergistic agent, wherein the dispersing auxiliary agent is tween 20, the adding amount of tween 20 is 0.01% -0.05% by volume, and the active components further comprise trehalose.
5. A biocontrol formulation as claimed in claim 3 in the form of a wettable powder or microcapsule suspension in water.
6. The biocontrol formulation of claim 3, wherein the total concentration of viable bacteria in the biocontrol formulation is 1 x 10 7 -1×10 9 CFU/mL.
7. A preparation method of bacterial fermentation broth for resisting rice sheath blight is characterized by comprising the step of culturing the bacillus composts A1 according to claim 1 to obtain the fermentation broth.
8. The method according to claim 7, which comprises inoculating Bacillus composts A1 strain to LB liquid medium at 28-35deg.C to obtain strain fermentation broth with OD600 value of (0.7-1.2).
9. A seed fermentation broth prepared by the preparation method of claim 7 or 8.
10. A method for preventing sheath blight of rice, which is characterized by comprising the step of spraying the strain fermentation liquor according to claim 9 on rice leaves in four-leaf stage and/or tillering stage and/or booting stage of rice, wherein the spraying amount of the strain fermentation liquor is (4-6) ml/plant.

Description

Bacillus composts A1 and application thereof in preparation of preparation for improving rice sheath blight resistance Technical Field The invention belongs to the field of microbial pesticides and biological control of plant diseases, and relates to bacillus composts A1, in particular to application of bacillus composts A1 in preparation of a preparation for improving rice sheath blight resistance. Background Rice sheath blight is a fungal disease caused by infection of Rhizoctonia solani (Rhizoctonia solani AG 1-IA), and results in 10-30% yield loss worldwide every year. The disease mainly damages leaf sheath and leaf blade, and forms dark green water stain-shaped elliptic disease spots on the leaf sheath near the water surface in the early stage of disease, gradually expands into moire shape, and generates white hyphae and dark brown sclerotium when the humidity is high. At present, agricultural measures are combined with chemical prevention and control, the agricultural prevention and control comprises the steps of sclerotium salvaging, reasonable close planting and water and fertilizer management, and the chemical prevention and control only depend on chemical agents, mainly comprise triazole and succinate dehydrogenase inhibitors, and are easy to induce drug resistance, pesticide residues and ecological risks. Therefore, biological control technology of rice sheath blight disease has become a research hot spot in recent years. Biological control refers to a method of controlling pest animal and plant populations or reducing the extent of their damage by using organisms or their metabolites. Compared with the traditional treatment method, the biological prevention and control technology has the main advantages of small environmental damage and high specificity, and has the difficulty and key points of searching functional microorganisms with high efficiency, stability and harmlessness. Bacillus composts are members of bacillus which quickly colonize in a humus environment and can produce antibacterial lipopeptides and plant hormones, but the prior art is mainly focused on promoting composting or soil improvement, and is not used for preventing and definitely controlling rice sheath blight She Miansheng and has no report of an immune activation mechanism. Disclosure of Invention Aiming at the defects existing in the prior art, the invention aims to provide bacillus composts A1 and application thereof in preparation of a preparation for improving rice sheath blight resistance, the strain has the characteristics of high efficiency, stability and the like, solves the problems of low reproduction rate and unstable effect of the existing biocontrol strain She Jiding, and remarkably inhibits the expansion of rice sheath blight bacterial plaque. In order to achieve the above purpose, the technical scheme of the invention is as follows: the invention provides a bacillus composts (Bacillus Stercoris) A1 for resisting rice sheath blight, which has a preservation number of CGMCC No.35058. The invention also provides application of the bacillus composts A1 in rice sheath blight resistance. The invention also provides a biocontrol preparation for resisting rice sheath blight, which comprises an active ingredient and an auxiliary ingredient, wherein the active ingredient comprises the bacillus composts A1 or fermentation liquid thereof. Preferably, the auxiliary components comprise a carrier, a dispersing auxiliary agent, a protective agent and a synergistic agent, wherein the dispersing auxiliary agent is Tween 20, the mass fraction of the Tween 20 is 0.01% -0.05%, and the active components further comprise seaweed oligosaccharide. Preferably, the dosage form of the biocontrol agent is wettable powder or microcapsule water suspension. Preferably, the total concentration of viable bacteria in the biocontrol agent is 1X 10 7-1×109 CFU/mL. The invention also provides a preparation method of the bacterial fermentation broth for resisting rice sheath blight, which comprises the step of culturing the bacillus composts A1 to obtain the fermentation broth. Preferably, the method comprises inoculating the bacillus composts A1 strain into LB liquid medium at 28-35 ℃ for culturing to obtain strain fermentation broth with OD600 value reaching (0.7-1.2). The invention also provides a strain fermentation broth prepared by the preparation method. The invention also provides a method for preventing the sheath blight of rice, which comprises the step of spraying the strain fermentation liquor on the leaf surfaces of the rice in the four-leaf stage and/or the tillering stage and/or the booting stage of the rice, wherein the spraying amount of the strain fermentation liquor is (4-6) ml/plant. In summary, compared with the prior art, the scheme of the invention has the following beneficial effects: The separated bacillus compost A1 can be used for preparing and obtaining the biocontrol preparation for the rice sheath blight, and the bi