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CN-121991838-A - Kelp endophyte listeria megaterium Priestia megaterium H403,403 and application thereof

CN121991838ACN 121991838 ACN121991838 ACN 121991838ACN-121991838-A

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to a kelp endophyte listeria megaterium Priestia megaterium H403,403 and application thereof. Aiming at the problems of long kelp seedling raising period and high cost, the invention separates an endophyte from kelp, and names the endophyte as Listeria megaly Priestia megaterium H403,403, and the co-culture experiment shows that the endophyte can obviously improve the germination rate and the growth rate of kelp seedlings, wherein the kelp length of a treatment group added with 14 multiplied by 10 6 cfu bacterial liquid reaches 3.6 times of that of a control group. The invention discloses a molecular mechanism of the giant listeria Priestia megaterium H for promoting the growth of kelp by multiple ways, provides a theoretical basis for developing a microorganism auxiliary seedling raising technology, is expected to reduce the kelp culture cost and promotes the sustainable development of industry.

Inventors

  • YE NAIHAO
  • WANG WEI
  • ZHANG XIAOWEN
  • YANG XIAOKUN
  • WANG YITAO
  • ZHANG PENGYAN
  • XU DONG
  • FAN XIAO
  • SUN KE
  • Wang Lepu

Assignees

  • 中国水产科学研究院黄海水产研究所

Dates

Publication Date
20260508
Application Date
20260121

Claims (10)

  1. 1. The kelp endophyte listeria megaterium Priestia megaterium H403,403 is characterized in that the strain is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2025, 08 and a preservation number of CGMCC No.35552.
  2. 2. The kelp endophyte listeria megaterium Priestia megaterium H403,403 of claim 1, wherein said kelp endophyte listeria megaterium Priestia megaterium H403,403 is isolated from kelp tissue.
  3. 3. The endophyte listeria megaterium Priestia megaterium H403,403 of claim 1, wherein the genomic sequence of the endophyte listeria megaterium Priestia megaterium H403,403 comprises a plurality of functional gene clusters associated with plant life-promoting properties, including a phosphate transport system gene cluster, a nitrogen fixation related gene cluster, a siderophore biosynthesis gene cluster, and an indole-3-acetic acid synthesis pathway related gene cluster.
  4. 4. The kelp endophyte listeria megaterium Priestia megaterium H403,403 of claim 1, wherein said kelp endophyte listeria megaterium Priestia megaterium H403,403 is capable of secreting siderophores, dissolving organic phosphorus, and producing indole-3-acetic acid under tryptophan induction.
  5. 5. The kelp endophyte listeria megaterium Priestia megaterium H403,403 of claim 1, wherein said kelp endophyte listeria megaterium Priestia megaterium H403,403 is adapted to a 10-25 ℃ 1-5% salinity environment and does not have alginate degrading capabilities.
  6. 6. Use of the kelp endophyte listeria megaterium Priestia megaterium H403,403 of claim 1 in algae cultivation.
  7. 7. The use according to claim 6, wherein the seaweed is kelp.
  8. 8. The use according to claim 7, wherein said endophyte listeria megaterium Priestia megaterium H403,403 is used for growing seedlings of kelp.
  9. 9. A kelp seedling raising method, characterized in that kelp seedling raising is performed by using the kelp endophyte listeria megaterium Priestia megaterium H403,403 according to any one of claims 1-5.
  10. 10. The kelp seedling raising method as claimed in claim 9, wherein the seedling raising method specifically comprises the steps of inoculating listeria megaterium Priestia megaterium Storing H403 strain, immersing inoculating loop in bacterial solution, streaking onto LB solid culture substrate, and selecting Listeria megaly Priestia megaterium H403 colony, inoculating to liquid LB culture medium, centrifuging, washing with sterile seawater twice to obtain H403 strain, adding H403 strain into seawater, and culturing kelp seedling.

Description

Kelp endophyte listeria megaterium Priestia megaterium H403,403 and application thereof Technical Field The invention belongs to the technical field of microorganisms and aquaculture, and particularly relates to a kelp endophyte listeria megaterium Priestia megaterium H403,403 and application thereof. Background Algae cultivation is an important component of the aquaculture industry and plays a key role in maintaining the health of the marine ecosystem and promoting the development of blue economy. Kelp has covered about 1/4 of the coastline worldwide and is the second largest biological community in marine ecosystems that is second only to seaweed in scale. The technology of circulating cooling water seedling raising adopted from the 50 th century of the 20 th century is a standard production technology of the algae cultivation industry through cultivation of summer spores under continuous optimization and improvement. Although the technology solves the technical problem of high-temperature seedling raising in summer, the technology still faces remarkable cost pressure that firstly, the water temperature of the cultivation needs to be strictly controlled to be about 12 ℃, and secondly, the cultivation needs to be equipped with professional personnel for monitoring and managing the growth state every day. The whole seedling raising period usually lasts for more than 3 months, resulting in a great increase in production cost. The cost mainly comprises two major parts, namely, the power consumption is firstly that the statistical data show that the power consumption in the growing season of the seedlings is greatly increased by 80 times compared with that in the light season, the power consumption of monthly is up to 277,189 kWh, and the cost is secondly that the related research shows that the expenditure accounts for 12.8 percent of the total cost of the seedling production. Therefore, the cultivation cost can be reduced by shortening the cultivation time, and more profits can be obtained. In recent years, the application potential of microbial control technology in the field of algae cultivation is receiving a great deal of attention. At present, no mature case of kelp seedling culture by using a microbial technology in a large scale exists. Preliminary experiments show that Bacillus subtilis can be reintroduced into ulvasp by physical disruption, and the diversity and abundance of algal microbiota are found to be significantly altered. These studies have found that they provide an important reference for the future development of microorganism-assisted seedling techniques. Theoretical studies indicate that specific strains may promote seaweed growth by secreting phytohormone analogs or activating antioxidant systems, etc. Early growth of algae (during seedling culture) is more sensitive to colonization by microorganisms, and some macroalgae can even transfer beneficial microorganisms from colonization. With the penetration of microorganism-host interaction research, more and more evidence indicates that endophytes and algae can form a close reciprocal symbiotic relationship, and the growth, development and stress resistance of hosts are obviously promoted through various mechanisms. With reference to established Plant Growth Promoting (PGP) mechanism studies paradigms in terrestrial plants, endophytes typically promote host growth by nitrogen fixation, phytohormone synthesis (e.g., IAA), biocontrol (e.g., secretion of antibacterial substances), siderophore production, and enhanced mineral solubility. These mechanisms are also of great reference value in algae-microorganism interactions, particularly in the cultivation of large economic algae such as kelp. By analyzing the PGP function of the endophyte, a theoretical basis can be provided for developing a special microbial preparation for the aquatic product, so that the kelp seedling raising process is optimized, and the green and high-efficiency cultivation process is realized. The research thought not only expands the application of the terrestrial plant PGP mechanism in the field of aquatic products, but also provides a new research direction for algae-microbiome engineering. Disclosure of Invention In order to solve the problems of long kelp seedling raising period and high cost in the prior art, the invention provides a kelp endophyte listeria megaterium Priestia megateriumH403,403 and application thereof. In order to achieve the above purpose, the invention adopts the following technical scheme: The invention separates an endophytic bacterium from kelp, the endophytic bacterium is preserved in China general microbiological culture Collection center (China Committee for culture Collection of microorganisms) with a preservation date of 2025, 08 and 22 days, and a preservation number of CGMCC No.35552. This was designated listeria megaterium Priestia megateriumH403,403. The invention identifies key gene clusters related to IAA synthesis, siderophore pr