CN-121991839-A - A metagen prepared from Bifidobacterium longum CCFM1508 and having effect in regulating de novo synthesis of skin ceramide and improving skin health

Abstract

The invention discloses a metagen prepared from bifidobacterium longum CCFM1508 and capable of regulating and controlling de novo synthesis of skin ceramide to improve skin health, belonging to the technical field of microorganisms and medicines. The bifidobacterium longum CCFM1508 and the metazoan prepared by the bifidobacterium longum CCFM1508 have the effects of promoting the synthesis of ceramide and improving skin health in external use and oral administration, and are characterized in that the thallus lysate and fermentation supernatant of the bifidobacterium longum CCFM1508 can promote the secretion of ceramide by HaCaT cells in external use, wherein the thallus lysate can obviously improve the activity of the HaCaT cells. The effect of the viable bacteria and metazoan of the oral bifidobacterium longum CCFM1508 on the skin appearance index, the nerve acyl content and the pro-inflammatory factors is obvious. Therefore, the bifidobacterium longum has great application prospect in preparing external or oral medicines for promoting the synthesis of skin ceramide and improving skin health and preparing foods, health products or cosmetics.

Inventors

• CUI SHUMAO
• XIN LINA
• MAO BINGYONG
• TANG XIN
• YANG BO
• YAO QINGWEI

Assignees

• 无锡特殊食品与营养健康研究院有限公司

Dates

Publication Date

20260508

Application Date

20260122

Claims (10)

1. 1. A bifidobacterium longum (Bifidobacterium longum subsp. Longum) CCFM1508, said bifidobacterium longum CCFM1508 having been deposited at the Cantonese province microorganism strain deposit under the accession number GDMCC No:66744, month 7 and 24 of 2025.
2. 2. A microbial preparation comprising the bifidobacterium longum CCFM1508 of claim 1.
3. 3. A metagen prepared from bifidobacterium longum CCFM1508 as claimed in claim 1 wherein the metagen is any of (a) - (c): (a) Fermenting the bifidobacterium longum CCFM1508, and collecting fermentation supernatant; (b) Thermally treating the bifidobacterium longum CCFM1508 bacteria to obtain inactivated bacteria; (c) Re-suspending the bifidobacterium longum CCFM1508, homogenizing under high pressure, centrifuging, taking supernatant, and performing heat treatment to obtain a thallus lysate; The fermentation supernatant or the thallus lysate is liquid or powder obtained after freeze-drying.
4. 4. A product comprising a bifidobacterium longum CCFM1508 as claimed in claim 1 or a microbial agent as claimed in claim 2 or a metazoan as claimed in claim 3.
5. 5. The product of claim 4, wherein the product comprises a food product, a pharmaceutical product, a health product, or a commodity product.
6. 6. The product of claim 5, wherein the daily chemical product comprises a skin care product, a cosmetic product, or a cleaning product, and wherein the daily chemical product does not contain living bacteria.
7. 7. The product of claim 5, wherein the daily chemical product comprises a powder, gel, emulsion, paste, or solid formulation.
8. 8. The product of claim 5, wherein the pharmaceutical product is an oral pharmaceutical product or an external pharmaceutical product.
9. 9. Use of bifidobacterium longum CCFM1508 as claimed in claim 1 or the microbial preparation as claimed in claim 2 or the metazoan as claimed in claim 3 in the manufacture of a medicament or commodity product having skin care effect, wherein the skin care effect is to increase skin ceramide content and/or enhance skin water retention.
10. 10. Use of bifidobacterium longum CCFM1508 as claimed in claim 1 or a microbial agent as claimed in claim 2 or a metazoan as claimed in claim 3 in the preparation of a ceramide or a ceramide-containing product.

Description

A metagen prepared from Bifidobacterium longum CCFM1508 and having effect in regulating de novo synthesis of skin ceramide and improving skin health Technical Field The invention relates to a metagen prepared from bifidobacterium longum CCFM1508 and capable of regulating and controlling de novo synthesis of skin ceramide to improve skin health, belonging to the technical field of microorganisms and medicines. Background The skin is the largest organ of the human body and is the first physical barrier against external environmental stimuli and to prevent water loss. The integrity of its barrier function is critical and its impaired function is associated with a variety of skin problems such as dryness, itching, sensitivity, atopic dermatitis and the like. In the skin barrier function, the Ceramide (CERAMIDES) filled in the stratum corneum space plays an irreplaceable core role, accounting for about 50% or more of intercellular lipids. The ceramide forms a compact double-layer lipid structure through orderly arrangement with cholesterol, free fatty acid and the like, thereby effectively locking moisture, maintaining skin steady state and resisting external invasion. The synthesis of skin ceramide mainly comprises a slave synthesis pathway, a sphingomyelinase pathway and a salvage pathway, wherein the slave synthesis pathway is a main pathway for maintaining the balance of skin ceramide. Studies have shown that there are significant abnormalities in the content and composition ratio of ceramide in the epidermis of patients with dry skin, aging and various dermatitis diseases. Therefore, the skin ceramide can be effectively supplemented in time, the skin health can be effectively improved, and the development of related skin inflammation can be prevented. At present, strategies aimed at supplementing ceramide to improve skin problems are mainly classified into (1) exogenous supplementation, i.e., direct addition of ceramide to cosmetic or external preparations. The method has obvious limitations, most of externally added ceramide is artificially synthesized, the acting effect is doubtful, the transdermal absorption efficiency is low, and the ceramide is difficult to effectively penetrate into the deep part of the skin stratum corneum, and (2) endogenous promotion is that the ceramide is synthesized by stimulating skin cells per se through active ingredients. Common methods include the use of some plant extracts (e.g., sphingolipids), vitamins (e.g., niacinamide), and the like. However, these ingredients have varying effects, an ambiguous mechanism of action, and may be irritating to a portion of the skin-sensitive population. Thus, promoting synthesis of endogenous ceramides in the skin, especially the de novo synthesis pathway, is considered one of the key strategies to maintain skin barrier, improving skin health. In recent years, the concept of metaverse has attracted considerable attention in the field of skin health. Metazoan refers to preparations of inanimate microorganisms and/or components thereof that are beneficial to host health, including cell components, metabolites, cell lysates, and the like. Some studies have found that specific lactic acid bacteria or bifidobacteria fermentation lysates have the potential to improve skin moisture and reduce inflammation. However, most of the existing researches are in macroscopic appearance (such as moisturizing and anti-inflammatory), the action mechanism of the existing researches is often vague, and particularly in regulating and controlling the synthesis of skin ceramide, few reports are related, and most of the existing researches are external emulsions containing metacomponents. The absorption and action effects of the skin-care product stay on the appearance of the skin. At present, a specific bifidobacterium longum strain has not been studied and reported, and the prepared metagen can realize endogenous regulation of skin ceramide synthesis and improve skin health by regulating and controlling the expression of key enzymes (such as serine palmitoyl transferase SPT, ceramide synthase CerS and the like) in the de novo synthesis pathway of skin ceramide. Therefore, there is an urgent need in the art to develop a metaplastic with definite efficacy, clear mechanism, safety and stability, which can effectively activate the endogenous biosynthesis pathway of skin ceramide, and provide a new solution for developing functional cosmetics, skin care products or medical auxiliary products for improving skin dryness, barrier damage and related skin problems. At present, the related research of endogenous regulation and control of natural ceramide synthesis and regulation and control pathway research of probiotics or metazoans through oral administration is less, for example, CN115141783A discloses that a strain of Sawau lactobacillus sake subspecies can maintain and repair skin barriers, regulation and control related inflammatory factors improve skin health, but n