CN-121991841-A - Sinorhizobium meliloti and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and discloses sinorhizobium meliloti and application thereof. The invention provides a sinorhizobium meliloti, which is classified and named Sinorhizobium meliloti and preserved in China general microbiological culture collection center (CGMCC) No.36886. The sinorhizobium meliloti provided by the invention can degrade the avermectin with high efficiency, is obviously higher than a plurality of reported degradation strains, shows excellent degradation efficiency and environmental adaptability, can effectively degrade the avermectin in soil, and can realize degradation of the avermectin in grape fruits.

Inventors

• LIU FENGJUAN
• HE WEIZHONG
• LIAN WEIJIA
• DUAN XIAOMAN
• HUANG WEI
• ZHANG XUETONG
• WANG CHENG
• DU JIANGTAO
• FAN YINGYING
• XU ZHENZHEN
• LI BINBIN
• CUI XING

Assignees

• 新疆维吾尔自治区农业科学院

Dates

Publication Date

20260508

Application Date

20260130

Claims (10)

1. 1. The sinorhizobium meliloti is characterized in that the sinorhizobium meliloti is classified and named Sinorhizobium meliloti and is preserved in China general microbiological culture collection center with the preservation number of CGMCC No.36886.
2. 2. Use of sinorhizobium meliloti according to claim 1 for degrading abamectin.
3. 3. The use according to claim 2, characterized in that the degradation environment has a pH of 6.0-8.0, a temperature of 25-40 ℃, and an avermectin concentration of 20-100 mg/L.
4. 4. Use according to claim 3, characterized in that the degradation environment has a pH of 7.0, a temperature of 35 ℃ and an avermectin concentration of 60mg/L.
5. 5. A microbial agent comprising the sinorhizobium meliloti and/or a supernatant, a fermentation product, a filtrate or an extract of the sinorhizobium meliloti and/or a culture thereof according to claim 1 as an active ingredient. .
6. 6. A preparation for degrading abamectin in environment, which is characterized in that the effective component comprises the ernatant, fermentation product, filtrate or extract of sinorhizobium meliloti and/or a culture thereof according to claim 1.
7. 7. A method for reducing the concentration of abamectin in an environment, comprising allowing sinorhizobium meliloti according to claim 1 to act on an environment containing abamectin.
8. 8. The method of reducing abamectin concentration in an environment of claim 7, wherein said environment is soil, water or fruit.
9. 9. The method of reducing the concentration of abamectin in an environment according to claim 7, wherein in the environment containing abamectin, the pH is 6.0-8.0, the temperature is 25-40 ℃, and the concentration of abamectin is 20-100 mg/L.
10. 10. The method of reducing the concentration of abamectin in an environment according to claim 9, wherein said environment containing abamectin is soil, the pH is 7.0, the temperature is 35 ℃, and the concentration of abamectin is 60mg/L.

Description

Sinorhizobium meliloti and application thereof Technical Field The invention belongs to the technical field of microorganisms, and discloses sinorhizobium meliloti and application thereof. Background Avermectin (AVERMECTIN, AVM) is a fat-soluble macrolide insecticide, stable in property at normal temperature, and easy to dissolve in organic solvent and difficult to dissolve in water. The excellent insecticidal activity makes the insecticidal composition widely applied to global agriculture, but the insecticidal composition is also continuously remained and accumulated in the environment due to strong fat solubility and difficult natural degradation. These residues not only induce microbial resistance, but also pose a potential threat to the ecosystem and human health. At present, various technologies for removing avermectin residues have been developed, mainly comprising photocatalytic degradation, thermal decomposition, microbial degradation and the like. Among them, microbial degradation is attracting attention due to its low cost and environmentally friendly nature. The presently reported functional microorganisms involve several genera, such as strains of Bacillus spp and Burkholderia sp, which have been demonstrated to degrade directly with abamectin as a carbon source. However, these natural strains generally face bottlenecks such as limited degradation efficiency, weak environmental adaptability and insufficient application stability, and these bottlenecks have been generally observed and reported in fields and fruit applications of pesticide degradation microbial inoculum, which severely restrict the practical large-scale application thereof. Therefore, the natural degrading bacteria with strong adaptability to the excavation environment, high degradation capacity and wide application range are important for developing high-efficiency biological bacteria. Disclosure of Invention In order to overcome the problems in the prior art, the invention provides sinorhizobium meliloti and application thereof. Compared with the prior art, the invention has the advantage of providing the sinorhizobium meliloti TLF6-3 which is derived from rhizobium and has high-efficiency abamectin degradation capability for the first time. The strain not only has inherent plant symbiotic characteristics and environmental adaptability of rhizobia, but also has a remarkable degradation function on abamectin, and widens the resource range of pesticide degrading bacteria. The strain has high degradation efficiency, the degradation rate of avermectin under experimental conditions is obviously superior to that of most reported degrading bacteria, and the strain has excellent pesticide decomposition potential. The strain TLF6-3 has the advantages of rapid growth and propagation, strong stress resistance, capability of maintaining degradation activity under various environmental conditions and good field application potential. The strain is nontoxic and harmless, can be directly or prepared for degrading abamectin residues of agricultural soil, water, fruits and the like, and has dual functions of increasing yield and quality of agriculture and protecting environment. In the technical scheme provided by the invention, "TLF6-3", "bacterial strain TLF6-3" or "Sinorhizobium meliloti TLF6-3" and "avermectin degrading bacteria" all represent Sinorhizobium meliloti provided by the invention. In one aspect, the invention relates to a plant of Sinorhizobium meliloti, the preservation information of which is as follows: The name of the strain is Sinorhizobium meliloti TLF6-3; classified naming, sinorhizobium meliloti Sinorhizobium meliloti; The preservation time is 2025, 12 months and 04 days; The preservation unit is China general microbiological culture Collection center; the preservation number is CGMCC No.36886; Address of the Qingyang district North Star, west Song No. 1, 3 in Beijing city. In another aspect, the invention relates to the use of said sinorhizobium meliloti for degrading avermectin. Further, in the application provided by the invention, the pH of the degradation environment is 6.0-8.0, the temperature is 25-40 ℃, and the concentration of abamectin is 20-100 mg/L. Further, in the application provided by the invention, the pH of the degradation environment is 7.0, the temperature is 35 ℃, and the avermectin concentration is 60mg/L. In another aspect, the present invention relates to a microbial agent, the active ingredient of which comprises the supernatant, fermentation product, filtrate or extract of sinorhizobium meliloti and/or its culture. In another aspect, the invention relates to a preparation for degrading abamectin in the environment, the active ingredients of which comprise the supernatant, fermentation product, filtrate or extract of sinorhizobium meliloti and/or the culture thereof. Any of the sinorhizobium meliloti provided herein, or any of its active variants, e.g., combinations of hyphae, can be formulated as