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CN-121991844-A - Low-Wen Wanji-resistant polycyclic aromatic hydrocarbon degrading bacterium and application thereof in groundwater remediation with combined pollution

CN121991844ACN 121991844 ACN121991844 ACN 121991844ACN-121991844-A

Abstract

The invention relates to a low-resistance Wen Wanji polycyclic aromatic hydrocarbon degrading bacterium and application thereof in restoration of groundwater with combined pollution, wherein the low-resistance Wen Wanji polycyclic aromatic hydrocarbon degrading bacterium is named Novosphingobium sp. Compared with the prior art, the Novosphingobium sp.SAF strain can degrade alkyl polycyclic aromatic hydrocarbon such as methylnaphthalene, 1-methyl phenanthrene and the like, non-alkyl polycyclic aromatic hydrocarbon such as naphthalene, phenanthrene, pyrene and the like and heterocyclic aromatic hydrocarbon such as dibenzofuran and the like at the temperature of 10-28 ℃, has the property of efficiently degrading various aromatic pollutants at low temperature, and is beneficial to solving the problems of tailing and rebound and the like of groundwater restoration in petrochemical polluted sites.

Inventors

  • Cui changzheng
  • GU FEI
  • LUO YUKE
  • OUYANG JI

Assignees

  • 华东理工大学

Dates

Publication Date
20260508
Application Date
20260204

Claims (10)

  1. 1. A low-Wen Wanji-resistant polycyclic aromatic hydrocarbon degrading bacterium is characterized in that the bacterium is named Novosphingobium sp.SAF and is preserved in China Center for Type Culture Collection (CCTCC) M20253008, and the preservation time is 2025, 12 months and 25 days.
  2. 2. The use of the low Wen Wanji resistant polycyclic aromatic hydrocarbon degrading bacteria according to claim 1 in the remediation of groundwater with combined pollution.
  3. 3. The use of claim 2, wherein the low Wen Wanji-resistant polycyclic aromatic hydrocarbon degrading bacteria are used for in situ degradation of contaminants in the co-contaminated groundwater, the contaminants including alkyl polycyclic aromatic hydrocarbons, non-alkyl polycyclic aromatic hydrocarbons, heterocyclic aromatic hydrocarbons.
  4. 4. The use according to claim 3, wherein the alkyl polycyclic aromatic hydrocarbon is selected from any one or more of methylnaphthalene, 1-methylphenanthrene, 4-ethylbiphenyl; The non-alkyl polycyclic aromatic hydrocarbon compound is selected from any one or more of naphthalene, phenanthrene and pyrene; The heterocyclic aromatic compound is dibenzofuran.
  5. 5. The application of claim 3, wherein the working temperature of the low Wen Wanji polycyclic aromatic hydrocarbon degrading bacteria is 10-28 ℃ when in-situ degrading the pollutants in the composite polluted groundwater.
  6. 6. The use according to claim 3, wherein the low Wen Wanji polycyclic aromatic hydrocarbon degrading bacteria has a working pH of 6.0-9.0 when degrading contaminants in the co-contaminated groundwater in situ.
  7. 7. The use according to claim 3, wherein the low Wen Wanji polycyclic aromatic hydrocarbon degrading bacteria have an initial contaminant concentration of no more than 80 mg/L when in situ degrading contaminants in the co-contaminated groundwater.
  8. 8. A product for degrading a contaminant in a co-contaminated groundwater, comprising the low Wen Wanji-resistant polycyclic aromatic hydrocarbon degrading bacterium according to claim 1, wherein the contaminant comprises an alkyl polycyclic aromatic hydrocarbon compound, a non-alkyl polycyclic aromatic hydrocarbon compound, and a heterocyclic aromatic hydrocarbon compound.
  9. 9. The method for degrading the pollutants in the composite polluted groundwater is characterized by comprising the following steps of: the low Wen Wanji-resistant polycyclic aromatic hydrocarbon degrading bacteria in the invention as set forth in claim 1 are inoculated into the ground water with complex pollution for degradation.
  10. 10. The method of claim 9, wherein the OD 600nm value of the low Wen Wanji polycyclic aromatic hydrocarbon degrading bacteria in the contaminated groundwater is not less than 0.2, the degradation temperature is 10-28 ℃, the pH is 6.0-9.0, and the initial concentration of the pollutant is not more than 80 mg/L.

Description

Low-Wen Wanji-resistant polycyclic aromatic hydrocarbon degrading bacterium and application thereof in groundwater remediation with combined pollution Technical Field The invention belongs to the technical field of environmental pollution microbial remediation, and relates to a low Wen Wanji-resistant polycyclic aromatic hydrocarbon degrading bacterium and application thereof in groundwater remediation with combined pollution. Background Groundwater systems, as a key resource for maintaining ecological balance, are often severely threatened by non-aqueous liquid (NAPLs) organic contaminants. NAPLs contaminants are deposited in the form of droplets or films in the subsurface environment in soil pores with very strong concealment and persistence. In the existing pollution site treatment, although the physical extraction technology can effectively remove free phase NAPLs, the removal effect of residual phase NAPLs pollutants bound by capillary force is poor, a long-term tailing phenomenon usually occurs, rapid rebound of pollutant concentration easily occurs repeatedly after extraction is stopped, wherein alkyl polycyclic aromatic hydrocarbon (ALKYLATED POLYCYCLIC AROMATIC HYDROCARBONS, A-PAH) is used as a main component with high toxicity and high carcinogenic risk in NAPLs, and the continuous dissolution of the alkyl polycyclic aromatic hydrocarbon in underground water becomes a key bottleneck for restricting the site to reach the standard for restoration. The above problems are mainly due to the difficulty in fundamentally reducing the residual pollution source items by physical means, and in recent years, the degradation of key pollution components in NAPLs residual phases by in-situ bioremediation is considered as a preferred option for achieving green sustainable remediation of groundwater. The formation of a-PAHs is multi-sourced, not only from the natural association of Polycyclic Aromatic Hydrocarbon (PAHs) precursors with alkyl side chains in crude oil, but also from the more widespread incorporation of alkyl side chains onto PAHs precursor structures by subterranean anaerobic microorganisms during the metabolism of petroleum hydrocarbons, resulting in a sustained accumulation of a-PAHs in contaminated systems and the manifestation of significant characteristics. In petroleum pollution sites, the detection rate of the A-PAHs is high, and the content of the A-PAHs can even account for more than 98 percent of the total PAHs content. Among them, the bicyclic and tricyclic A-PAHs represented by methylnaphthalene and 1-methylphenanthrene are most widely distributed, and the abundance of them in groundwater NAPLs is often an order of magnitude higher than that of corresponding non-alkyl PAHs. The a-PAHs exhibit greater bioaccumulation and environmental persistence than the 16 PAHs (naphthalene, indeno [1,2,3-cd ] pyrene, acenaphthylene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo [ a ] anthracene, chrysene, benzo [ b ] fluoranthene, benzo [ k ] fluoranthene, benzo [ a ] pyrene, dibenzo [ a, h ] anthracene, benzo [ ghi ] perylene) that are preferentially monitored by the united states Environmental Protection Agency (EPA). Due to the introduction of alkyl substituent, the chemical substance has reduced water solubility and enhanced hydrophobicity, so that the chemical substance is more easily accumulated in organisms, and further, a higher biotoxic effect is induced. Studies show that methylphenanthrene has 2-5 times higher potency in activating human aromatic hydrocarbon receptors (AhR) than non-alkyl phenanthrenes, resulting in significant increases in risk of genotoxicity, carcinogenicity, and the like. In addition, the natural degradation path of the pollutants is blocked due to the limitation of the underground anoxic environment and the stable chemical structure of the A-PAHs, and the ecological safety of underground water forms a long-term serious challenge. However, the existing bioremediation research for polycyclic aromatic hydrocarbons is still mainly focused on non-alkyl PAHs (naphthalene, anthracene, fluoranthene, benzo [ b ] fluoranthene, benzo [ a ] pyrene have been definitely limited in the underground water quality standard (GB/T14848-2017), naphthalene is simultaneously incorporated into the priority control chemical directory (first batch) of China), the efficient repair research on A-PAHs is relatively deficient, only a small number of research reports on A-PAHs degradation strains are provided, but the degradation is generally greatly influenced by the growth environment, and particularly degradation is inhibited under the actual scenes of low temperature, combined pollution and the like, and the efficiency is remarkably reduced. For example, while Sphingobium sp.MP 9-4 strain has degradation ability for 1-methylphenanthrene, its reaction conditions are strictly limited to laboratory constant temperature environment of 28 ℃ and can only process 9.5 mg/L of 1-methylph