CN-121991845-A - Monomonas bentonite oligotrophic KG93 and application thereof

Abstract

The invention provides a bentonite stenotrophomonas KG93 and application thereof, belonging to the technical field of agricultural microorganisms. The bentonite stenotrophomonas KG93 of the invention is preserved in China general microbiological culture Collection center (CGMCC) No.37256. The strain is separated from healthy bitter gourd rhizosphere soil in a leek-bitter gourd interplanting mode, and experiments show that the inhibition rate of the strain on colony growth of fusarium oxysporum bitter gourd specialized type bitter gourd which is pathogenic fungus of bitter gourd fusarium wilt reaches 86.06%, and the prevention and treatment effects on bitter gourd fusarium wilt in natural soil and sterilized soil are 73.91% and 36.05% respectively. The inoculation strain KG93 can obviously reduce the disease index, improve the chlorophyll content of balsam pear leaves, strengthen photosynthesis, promote plant growth and promote the enzymatic activity of soil. The strain KG93 has good application potential in biological control of the fusarium wilt of the bitter melon.

Inventors

• HAN XU
• HE HONG
• YANG YAN
• NIU YU
• YU RENBO
• LIU ZHAOHUA

Assignees

• 中国热带农业科学院热带作物品种资源研究所

Dates

Publication Date

20260508

Application Date

20260205

Claims (10)

1. 1. The stenotrophomonas bentonite (Stenotrophomonas bentonitica) KG93 is characterized in that the stenotrophomonas bentonite is preserved in China general microbiological culture Collection center (CGMCC) No.37256, the preservation time is 2025, 12 and 31 days, and the preservation address is North Star Xiya No. 1,3 of the Chaiyang region of Beijing city.
2. 2. A microbial agent, characterized in that an active ingredient in the microbial agent is the stenotrophomonas bentonite KG93 of claim 1.
3. 3. The microbial agent according to claim 2, wherein the effective viable count in the microbial agent is 1-9 x 10 7 CFU/mL.
4. 4. The preparation method of the bacterial liquid of the stenotrophomonas bentonite KG93, which is characterized by inoculating the stenotrophomonas bentonite KG93 into a fermentation medium for fermentation culture to obtain the bacterial liquid.
5. 5. The method according to claim 4, wherein the fermentation culture temperature is 26-30 ℃ and the rotation speed is 180-220 rpm.
6. 6. Use of stenotrophomonas bentonite KG93 as claimed in claim 1 in any one or more of the following: (1) Application in inhibiting fusarium oxysporum; (2) Application in preventing and treating plant wilt; (3) Use in promoting plant growth; (4) The application in improving the enzymatic activity of soil; (5) Application of the fusarium oxysporum inhibitor in preparation of a microbial inoculum for inhibiting fusarium oxysporum; (6) Application in preparing a microbial inoculum for preventing and treating plant fusarium wilt; (7) The application in preparing a microbial inoculum for promoting plant growth; (8) The application of the microbial inoculum in preparing the microbial inoculum for improving the enzymatic activity of soil.
7. 7. The use according to claim 6, wherein the plant comprises balsam pear.
8. 8. The use according to claim 6, wherein the enzyme preparation comprises soil urease, soil sucrase and soil acid phosphatase.
9. 9. A method for controlling plant blight, characterized in that plants are root-irrigated with a microbial agent comprising stenotrophomonas bentonite KG93 as defined in claim 1.
10. 10. The method of claim 9, wherein the plant comprises momordica charantia.

Description

Monomonas bentonite oligotrophic KG93 and application thereof Technical Field The invention relates to the technical field of biocontrol bactericides, in particular to a bentonite stenotrophomonas KG93 and application thereof. Background The fusarium wilt of bitter melon is an earth-borne disease caused by fusarium oxysporum balsam pear specialization (Fusarium oxysporum f.sp. momordicae), and has become one of the main factors affecting the output of balsam pear. The disease is transmitted through soil, pathogenic bacteria survive in the soil for a long time, infect the root system of balsam pear, cause the blockage of plant vascular bundles, wilt and death, and have great control difficulty. At present, the prevention and treatment of the fusarium wilt of the kumquat mainly depend on chemical pesticides, but the long-term use of the chemical pesticides is easy to cause the problems of pathogen resistance, soil microecological imbalance, environmental pollution and the like. Therefore, the development of an efficient and environment-friendly biological control method has important significance for sustainable development of the balsam pear industry. In recent years, soil-borne diseases are attracting attention in agricultural production, and screening of antagonistic microorganisms from plant rhizosphere soil has become an important way to develop strain-proofing. Plant rhizosphere soil is rich in abundant microbial resources, and the microorganisms play key roles in enhancing plant disease resistance, promoting plant growth, maintaining soil microecological balance and the like. Studies have shown that rhizosphere microorganisms are the first natural barrier against infection by soil borne pathogens. The biocontrol bacteria have the advantages of simple nutrition requirement, strong rhizosphere colonization capability, rapid propagation and the like, and have wide application prospect in crop disease control. For example, biocontrol strains such as Paenibacillus polymyxa, bacillus belicus and Bacillus brevis have achieved remarkable effects in controlling the wilt of crops such as tomatoes and cucumbers, and can reduce disease index, induce plant defense reaction and promote plant growth. Stenotrophomonas (Stenotrophomonas) microorganisms play an important role in the natural element circulation and have various biotechnological application potentials such as bioremediation, plant growth promotion and biocontrol. Among them, bentonite oligotrophic monad (Stenotrophomonas bentonitica) is a newly reported strain in recent years, and shows potential value in promoting plant growth and stress resistance, but its application in prevention and treatment of fusarium wilt of kumquat has not been reported yet. At present, the biological prevention and treatment research on the fusarium wilt of the kumquat is still limited, the prevention effect and the stability of the existing biocontrol strain are still to be further improved, and the related antagonism mechanism and the active substances are not clear. Therefore, aiming at the control requirement of the fusarium wilt of the bitter gourd, the efficient antagonistic strains are screened from the rhizosphere soil of the bitter gourd, the classification status, the biocontrol effect and the action mechanism of the efficient antagonistic strains are clear, and the efficient antagonistic strains have important theoretical and practical significance for developing novel biocontrol agents, reducing the use of chemical pesticides and guaranteeing the safe production of the bitter gourd. Disclosure of Invention In view of the above, the present invention provides a strain of stenotrophomonas bentonite KG93 and its application to solve the above problems. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a bentonite stenotrophomonas (Stenotrophomonas bentonitica) KG93 which is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of CGMCC No.37256, the preservation time of 2025 and 12 months and 31 days, and the preservation address of North Star Xidin No. 1 and 3 in the Chaoyang area of Beijing city. The invention provides a microbial agent, wherein the active ingredient in the microbial agent is the bentonite stenotrophomonas KG93 of claim 1. Preferably, the effective viable count in the microbial agent is 1-9×10 7 CFU/mL. The invention also provides a preparation method of the bacterial liquid of the stenotrophomonas bentonite KG93, which is characterized in that the stenotrophomonas bentonite KG93 is inoculated into a fermentation medium for fermentation culture, so as to obtain the bacterial liquid. Preferably, the temperature of the fermentation culture is 26-30 ℃, and the rotating speed is 180-220 rpm. The invention also provides application of the stenotrophomonas bentonite KG93 in any one or more of the following: (1) Application in inh