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CN-121991846-A - Phosphate-dissolving nitrogen-fixing carburetion functional bacterium and application thereof in soil-bearing growth promotion

CN121991846ACN 121991846 ACN121991846 ACN 121991846ACN-121991846-A

Abstract

The invention provides a phosphate-dissolving nitrogen-fixing carburant functional bacterium and application thereof in soil-bearing growth promotion, belonging to the technical field of agricultural microorganisms. The Acer ginnala ZC-7 is preserved in China center for type culture Collection with the preservation number of CCTCC NO: M20252883, the preservation time of 2025, 12 months and 15 days, and the preservation address of Wuhan university. The strain has effects of dissolving phosphorus, fixing nitrogen and producing IAA. In addition, the strain has wide carbon source utilization spectrum, has resistance to various chemical substances and can adapt to various soil environments. The strain can obviously promote the growth of corn plants and improve the corn yield, and field experiments show that the application of the microbial agent containing the strain can reduce the use of chemical fertilizers and improve the corn yield by 27%. The strain has comprehensive functions and strong adaptability, provides technical support for green agricultural development, and has important application value.

Inventors

  • WANG HAIZHEN
  • ZHOU YAO
  • XU JIANMING
  • YANG YULING
  • SUN HONGXIN

Assignees

  • 浙江大学

Dates

Publication Date
20260508
Application Date
20260205

Claims (10)

  1. 1. A Acer ginnala (Rahnella sp.) ZC-7 is characterized by being preserved in China center for type culture Collection (CCTCC NO: M20252883), wherein the preservation time is 2025, 12 months and 15 days, and the preservation address is Wuhan university.
  2. 2. A microbial agent, which is characterized in that the active ingredient of the microbial agent is Acer maple ZC-7 as described in claim 1.
  3. 3. The microbial agent according to claim 2, wherein the microbial agent is a liquid microbial agent, and the effective viable count in the liquid microbial agent is 1-9 x 10 9 CFU/mL.
  4. 4. The method for preparing the microbial agent according to claim 3, wherein the Acer ginnala ZC-7 is inoculated in a fermentation medium for fermentation culture to obtain the microbial agent.
  5. 5. The method according to claim 4, wherein the fermentation culture temperature is 28-32 ℃ and the rotation speed is 180-220 rpm.
  6. 6. Use of the rahnella acervulina ZC-7 of claim 1 in any one or more of the following: (1) Application in phosphate dissolution; (2) Use in the production of a nitrogen fixation enzyme; (3) Application in nitrogen fixation; (4) The application in the production of indoleacetic acid; (5) Use in promoting plant growth and/or increasing crop yield; (6) Application in soil fertility; (7) The application in preparing phosphate-dissolving bacteria agent; (8) The application in preparing the microbial inoculum for producing the azotase; (9) Application in preparing azotobacter; (10) The application in preparing the microbial inoculum for producing the indoleacetic acid; (11) The application in preparing a microbial inoculum for promoting plant growth and/or improving crop yield; (12) The application of the microbial inoculum with the soil fertility promoting effect is prepared.
  7. 7. The use according to claim 6, wherein the fertility of the soil is the improvement of the contents of available phosphorus, ammonium/nitrate nitrogen, soluble organic carbon/nitrogen and total organic carbon in the soil.
  8. 8. A method for promoting plant growth, characterized in that the microbial agent of the rahnella acervulina ZC-7 of claim 1 or the microbial agent of claim 2 is applied to soil of plant rhizosphere.
  9. 9. The method according to claim 8, wherein after the microbial agent is applied, the effective viable count of the Acer maple Rahnella ZC-7 in the soil of the plant rhizosphere is 0.8-1.2×10 6 CFU/g of soil.
  10. 10. The method of claim 8 or 9, wherein the plant comprises maize.

Description

Phosphate-dissolving nitrogen-fixing carburetion functional bacterium and application thereof in soil-bearing growth promotion Technical Field The invention relates to the technical field of agricultural microorganisms, in particular to a phosphorus-dissolving nitrogen-fixing carburetion functional bacterium and application thereof in soil-bearing growth promotion. Background Nitrogen and phosphorus are used as mineral elements necessary for plants, are important constituent components of various structural and functional substances such as proteins, nucleic acids, phospholipids, enzymes, plant hormones and the like in living cells of plants, and play an important role in the processes of plant energy metabolism, organic matter synthesis and metabolism, substance operation, gene expression and regulation, cell signal transduction and the like. The lack of nitrogen and phosphorus will inhibit the growth and development of plants, causing some hypo-vegetations and even failing to complete their life cycle. The application of the fertilizer not only increases the agricultural production cost, but also causes the selective accumulation of certain elements in the soil and the deterioration of the physicochemical properties of the soil, thereby causing environmental pollution. The microbial agent with multiple growth promoting functions is developed and utilized, and is an effective strategy for improving soil and promoting crop growth. Microorganisms play an important role in the soil nutrient circulation. In the prior art, there are many microbial sources with a single function. For example, phosphate solubilizing microorganisms can convert poorly soluble phosphorus in soil into an effective form, and nitrogen fixing microorganisms can fix nitrogen in the atmosphere as a plant available nitrogen source. In addition, some strains also have the ability to secrete plant growth hormone or antagonize pathogenic bacteria. However, the improvement of soil fertility is a system engineering, and the synergism of a plurality of nutrient elements such as nitrogen fixation, phosphorus dissolution, carburetion and the like is required. However, the existing commercial microbial inoculum generally has the problems of poor environmental adaptability, single function and the like, and the practical application effect is limited by the soil type, the climate condition and other multi-factor external conditions. Disclosure of Invention In view of the above, the invention provides a phosphate-dissolving nitrogen-fixing carburant functional bacterium and application thereof in soil-vortexing, the strain has the effects of phosphate dissolving, nitrogen-fixing, carburant and soil-vortexing, and the strain has strong environment adaptability, provides technical support for green agriculture development, and has important application value. In order to achieve the above object, the present invention provides the following technical solutions: The invention provides a Acer ginnala (Rahnella sp.) ZC-7 which is preserved in China center for type culture Collection, wherein the preservation number is CCTCC NO: M20252883, the preservation time is 2025, 12 months and 15 days, and the preservation address is Wuhan university. The invention also provides a microbial agent, and the active ingredient of the microbial agent is the Acer maple Rahnella ZC-7. Preferably, the microbial agent is a liquid microbial agent, and the effective viable count in the liquid microbial agent is 1-9×10 9 CFU/mL. The invention also provides a preparation method of the microbial agent, and the preparation method comprises the steps of inoculating the Acer ginnala ZC-7 in a fermentation medium for fermentation culture to obtain the microbial agent. Preferably, the temperature of the fermentation culture is 28-32 ℃, and the rotating speed is 180-220 rpm. The invention also provides application of the Acer ginnala ZC-7 in any one or more of the following: (1) Application in phosphate dissolution; (2) Use in the production of a nitrogen fixation enzyme; (3) Application in nitrogen fixation; (4) The application in the production of indoleacetic acid; (5) Use in promoting plant growth and/or increasing crop yield; (6) Application in soil fertility; (7) The application in preparing phosphate-dissolving bacteria agent; (8) The application in preparing the microbial inoculum for producing the azotase; (9) Application in preparing azotobacter; (10) The application in preparing the microbial inoculum for producing the indoleacetic acid; (11) The application in preparing a microbial inoculum for promoting plant growth and/or improving crop yield; (12) The application of the microbial inoculum with the soil fertility promoting effect is prepared. Preferably, the soil fertility efficacy is to increase the content of available phosphorus, ammonium/nitrate nitrogen, soluble organic carbon/nitrogen and total organic carbon in the soil. The invention also provides a method for pro