CN-121991848-A - Algicidal bacterial agent and preparation method and application thereof

Abstract

The invention discloses an algicidal bacterial agent, a preparation method and application thereof, and relates to the technical field of environmental microorganism and water treatment. The active ingredients of the algicidal agent comprise extracellular metabolites of bacillus licheniformis. The bacillus licheniformis with algae dissolving capability is obtained by separation. The extracellular metabolite of the strain is taken as a core active ingredient, and experiments prove that the strain has high-efficiency algae dissolving effect on various common algae such as microcystis aeruginosa, chlorella, sphingei algae, and the like, and breaks through the technical bottleneck of narrow algae dissolving spectrum of a single strain. The invention develops a novel algicidal agent based on the bacillus licheniformis, and the active ingredients of the algicidal agent have stable properties under the conventional storage condition and have high efficiency and environmental friendliness. The invention provides stable and reliable technical support for algae control in complex water environments and has wide application prospect.

Inventors

• BAI LONG
• JING SHUANGYI
• ZHANG ZHEKANG
• WANG ZHICHAO
• LI WEIPING
• LI GUOWEN
• LI REN
• MA MINGHUI
• HOU CHENLI
• YIN ZHENYU

Assignees

• 内蒙古科技大学

Dates

Publication Date

20260508

Application Date

20260211

Claims (10)

1. 1. The bacillus licheniformis (Bacillus licheniformis) with algae dissolving capability is characterized in that the bacillus licheniformis is preserved in the China general microbiological culture Collection center (China Committee) for culture Collection of microorganisms in 2025, 12 months and 12 days, the preservation address is North Star Xiyu No. 1,3 in the Chaoyang area of Beijing, and the preservation number is CGMCC No.37050.
2. 2. Use of the bacillus licheniformis of claim 1 for the preparation of algicidal agents.
3. 3. An algicidal agent, wherein the active ingredient comprises the extracellular metabolite of bacillus licheniformis of claim 1.
4. 4. The algicidal agent of claim 3, wherein the algicidal agent is in the form of a liquid formulation or a lyophilized powder formulation.
5. 5. The algicidal agent of claim 4, wherein the lyophilized powder further comprises a lyoprotectant.
6. 6. The algicidal agent of claim 5, wherein the lyoprotectant is trehalose, skim milk, or glycerol.
7. 7. The preparation method of the algicidal liquid preparation is characterized by comprising the following steps: fermenting and culturing the bacillus licheniformis of claim 1 to obtain a fermentation liquor, namely the algicidal liquid preparation.
8. 8. The preparation method of the algae dissolving freeze-dried powder is characterized by comprising the following steps: Fermenting and culturing the bacillus licheniformis of claim 1 to obtain a fermentation liquor; and adding a freeze-drying protective agent into the fermentation liquor, and performing freeze-drying treatment to obtain the algicidal freeze-drying powder.
9. 9. Use of the bacillus licheniformis of claim 1 or the algicidal agent of any of claims 3-6 for removing algae from a body of water.
10. 10. A method of removing algae from a body of water comprising the step of applying the bacillus licheniformis of claim 1 or the algicidal agent of any of claims 3-6 to the body of water to be treated.

Description

Algicidal bacterial agent and preparation method and application thereof Technical Field The invention relates to the technical field of environmental microorganism and water treatment, in particular to an algicidal bacterial agent, a preparation method and application thereof. Background Algae excessive proliferation caused by water eutrophication is a prominent technical problem facing the current water treatment field, and mass propagation of algae (algal bloom) can destroy the material circulation and energy flow of an aquatic ecosystem, so that the ecological structure of the water body is unbalanced. In the existing algae control technology, the physical method relies on means such as salvaging, ultrasonic and the like, has the technical limitations of high energy consumption, complex operation, difficult treatment of large-area water bodies and incapability of fundamentally inhibiting algae regeneration, and the chemical method realizes quick algae control by applying an algicide, but chemical agents are easy to remain in the water bodies to cause secondary pollution, and meanwhile, the chemical properties of the water bodies can be changed to influence the normal metabolism of aquatic organisms, and the algae can be induced to generate drug resistance after long-term use, so that the algae control effect is gradually attenuated. The microbial algae dissolving technology in the biological method has the advantages of strong environmental compatibility, outstanding specificity and the like, and becomes a research key in recent years, but the prior art still has a remarkable technical bottleneck. The single algae-dissolving strain has a narrow algae-dissolving spectrum, can only play a role on specific portal algae, is difficult to adapt to complex community structures where multiple algae coexist in an actual water body, has low algae-dissolving efficiency and long acting period, cannot meet the technical requirement of quick algae control in an algae outbreak period, has weak environmental adaptability, is easily affected in the conditions of water body temperature fluctuation, pH value change or nutrient substance competition and the like, and has unstable practical application effect, and in addition, the viable bacteria are easily inactivated in the production, processing, storage and transportation processes of the microbial inoculum, so that the effective components are degraded, and the large-scale application of the microbial inoculum is further limited. Therefore, development of algae dissolving technology with broad-spectrum algae dissolving activity, high effect, strong environmental adaptability and excellent stability becomes a key direction for breaking through the limitation of the prior art. Disclosure of Invention The invention aims to provide an algicidal bacteria agent, a preparation method and application thereof, so as to solve the problems in the prior art. The algicidal bacteria agent has high efficiency, broad-spectrum algicidal activity and excellent stability, provides stable and reliable technical support for algae control in complex water environments, and has wide application prospect. In order to achieve the above object, the present invention provides the following solutions: The invention provides a bacillus licheniformis (Bacillus licheniformis) with algae dissolving capability, which is preserved in the China general microbiological culture Collection center (China Committee) for culture Collection of microorganisms in 2025, 12 months and 3 rd edition of North Star Xiyu No. 1, the preservation address of which is the Chaiyang area of Beijing, and the preservation number of which is CGMCC No.37050. The invention also provides application of the bacillus licheniformis in preparation of algicidal bacteria. The invention also provides an algicidal agent, and the active ingredient comprises the extracellular metabolite of the bacillus licheniformis. Further, the algicidal agent is in the form of liquid preparation or freeze-dried powder. Further, the freeze-dried powder further comprises a lyoprotectant. Further, the lyoprotectant is trehalose, skimmed milk or glycerol. The invention also provides a preparation method of the algicidal liquid preparation, which comprises the following steps: and (3) fermenting and culturing the bacillus licheniformis to obtain a fermentation liquor, namely the algicidal liquid preparation. The invention also provides a preparation method of the algicidal freeze-dried powder, which comprises the following steps: fermenting and culturing the bacillus licheniformis to obtain fermentation liquor; and adding a freeze-drying protective agent into the fermentation liquor, and performing freeze-drying treatment to obtain the algicidal freeze-drying powder. The invention also provides application of the bacillus licheniformis or algicidal agent in removing algae in water. The invention also provides a water body algae removal method, which comprises