CN-121991852-A - Application of bacillus pseudolaris strain FD-6 in preparation of product for promoting growth of large-root glossoside and accumulating soluble protein content

Abstract

The application relates to application of a bacillus pseudolaris strain FD-6 in preparation of a product for promoting growth of large-root glossoside and accumulating soluble protein content, and belongs to the technical field of microorganisms. The microbial agent for promoting the growth of the large root of the dyers woad and promoting the accumulation of the soluble protein content of the large root of the dyer woad, disclosed by the application, has the active ingredient of viable bacteria of single bacillus pseudobacteria strain FD-6, and can be used for remarkably promoting the accumulation of the growth quantity, biomass and soluble protein content of the large root of the dyer woad She Qiguan. Wherein the preservation number of the bacillus pseudochinensis strain FD-6 is CCTCC NO: M20252238, which is preserved in China center for type culture Collection (China 10 month and 17 days) and classified and named as bacillus pseudochinensis FD-6 Fictibacillus sp.FD-6.

Inventors

• LI ZONGYAN
• WU LV
• LI YUNLONG
• LI MEIQIN
• LI YUANJUN

Assignees

• 西南林业大学

Dates

Publication Date

20260508

Application Date

20260226

Claims (5)

1. 1. The application of the bacillus pseudostell (Fictibacillus sp.) strain FD-6 in preparing a product for promoting the growth of the large root glossoside is provided, wherein the preservation number of the bacillus pseudostell FD-6 is CCTCC NO: M20252238, the bacillus pseudostell is preserved in the China center for type culture collection (China center for type culture collection) at 10 months of 2025, and the classification name is bacillus pseudostell FD-6 Fictibacillus sp. FD-6.
2. 2. The use according to claim 1, wherein said promoting growth of large root canal lingual blue comprises promoting root system growth, promoting leaf growth and/or increasing overall biomass.
3. 3. The application of the bacillus pseudostellatus strain FD-6 in preparing the product for promoting the accumulation of the soluble protein content of the large root glossoside is provided, wherein the preservation number of the bacillus pseudostellatus strain FD-6 is CCTCC NO: M20252238, the bacillus pseudostellatus strain FD-6 is preserved in the China center for type culture collection (China center for type culture collection) at 10 and 17 of 2025, and the classification is named as bacillus pseudostellatus FD-6 Fictibacillus sp.
4. 4. A microbial agent for promoting the growth of large root glossoside is characterized in that the active ingredient of the microbial agent is the living bacterium of single bacillus pseudolaris strain FD-6, the preservation number of the bacillus pseudolaris strain FD-6 is CCTCC NO: M20252238, the bacillus pseudolaris strain FD-6 is preserved in China center for type culture collection (China center for type culture collection) at 10 month 17 of 2025, and the class name is bacillus pseudolaris FD-6 Fictibacillus sp. FD-6.
5. 5. The microbial agent of claim 4, wherein the agent is used to promote accumulation of soluble protein content of the large root of glossodium falvescens.

Description

Application of bacillus pseudolaris strain FD-6 in preparation of product for promoting growth of large-root glossoside and accumulating soluble protein content Technical Field The invention relates to the technical field of microorganisms, in particular to application of a bacillus pseudolaris strain FD-6 in preparation of a product for promoting growth of large-root glossoside and accumulating soluble protein content. Background The large root of the glossopus macrophylla is a perennial epiphyte of the glossopus genus (Holcoglossum) of the orchidaceae (Orchidaceae), and the root of the glossopus macrophylla is rich due to the fact that the stem base wrapped by the leaf sheath has a plurality of bends. The large root of the glossopus macrophylla mainly grows on trunks in evergreen broad-leaved forests in mountain areas with the altitude of 1250-2000 meters, and is only distributed in the south to the west of Yunnan province in China. Large root of glossostrea is already listed in the washington Convention (CITES) -appendix II as limiting its international trade and is listed by the world natural protection consortium (IUCN) as a susceptible (VU) species, facing a higher risk of extinction. The national key protection wild plant directory clearly lists the whole genus of the hyoglossum plant as the first-class protection plant of the country, prohibits illegal mining and trading, and simultaneously the plant of the genus is listed in the national minimum population wild plant rescue protection engineering plan. More than 20 plants of the genus hyoglossum exist worldwide, 18 plants (the name of Chinese plant species 2025) are found and recorded in China, and partial new distribution is reported in the main distribution areas of Myma, thailand, laos and Vietnam. Very few studies have been conducted worldwide on the plants of the genus hygienics, including mainly the phylogenetic studies of the plants of the genus hygienics based on nuclear and chloroplast genes, and the taxonomic studies of the plants based on cell chromosomes. In addition, without any pollination medium or auxiliary, the large root of the glossodes radicata can rotate anthers through 360 degrees, and pollen blocks are precisely fed into the stigma cavity at the base of the coleus to complete fertilization, so that the glossodes radicata is evolved as a new propagation mechanism which is suitable for drought and lacks pollinating insect environments, and is reported by Nature in 2006. The plant of the genus hyoglossum has important scientific research value as an experimental material for researching the space-time distribution mode and the evolution process of the peculiar epiphyte of east Asia. Meanwhile, the flower has various colors and beautiful corolla shape, and becomes an important ornamental flower, thereby having great gardening development value. The soluble protein contains key enzymes in each growth period of plants, and the content of the soluble protein can directly reflect the growth vigor degree of the plants and the flowering potential of gardening flowers. In addition, part of soluble proteins are involved in the modeling of plant stress resistance properties, including Heat Shock Proteins (HSPs), osmotic regulatory proteins (such as dehydrins), antifreeze proteins (AFPs), metallothionein (MTs), etc., to improve the resistance of plants to high temperature, drought, cold, heavy metal stresses. For the large root glossostrea, the measurement of the content of the soluble protein is of great significance to plant physiological state evaluation, cultivation management and variety breeding, and especially the large root glossostrea is used as an important ornamental flower, and the content of the soluble protein is an important evaluation index in ornamental quality regulation and stress resistance management. The method can accelerate plant physiological metabolism, promote nitrogen utilization, regulate plant flowering in advance, enhance plant resistance and prolong the maintenance time of flower morphology by increasing the soluble protein content of the large root glossostrea majoris. The improvement of the content of the soluble protein has important indication effects on the aspects of screening of good varieties of large-root glossoside, early warning of stress injury, optimization of water and fertilizer management and the like. At present, no research and technical report about the breeding of the large root slot orchid exist at home and abroad, the invention aims to provide a single microbial inoculum for promoting the growth of the large root slot orchid leaf and the accumulation of the content of soluble protein, the effective growth of plants is realized through core microorganisms planted in the rhizosphere of the large root slot orchid, the synthesis of the soluble protein is promoted, the technical bottleneck of the important ornamental gardening flower breeding process is broken, and the technical support is p