CN-121991854-A - Streptomyces, microbial preparation and application thereof

Abstract

The invention relates to the field of microorganisms, in particular to streptomyces, a microbial preparation and application thereof. The present invention provides Streptomyces sp, deposited under the accession number: GDMCC 67625. The invention provides a novel streptomycete strain LE64, a preparation containing the strain and application thereof. The strain can effectively inhibit banana vascular wilt and greatly promote banana plant growth, and provides an environment-friendly microbial solution for solving the banana vascular wilt problem.

Inventors

• HU YONGHUA
• LV XIUJUN
• DENG NA
• GU HANJIE
• MO KUNLIAN

Assignees

• 中国热带农业科学院热带生物技术研究所

Dates

Publication Date

20260508

Application Date

20260304

Claims (10)

1. 1. Streptomyces sp, characterized in that it has a deposit number GDMCC 67625.
2. 2. A microbial agent characterized by comprising any one of the following and acceptable adjuvants: (a) Streptomyces sp according to claim 1, and/or (B) An inactivated strain of Streptomyces sp according to claim 1, and/or (C) A metabolite, derivative, fermentation broth, culture, exosome, lysate or extract of Streptomyces sp.
3. 3. A fertilizer or a fertilizer additive, comprising the Streptomyces sp according to claim 1 and/or the microbial agent according to claim 2 and an acceptable adjuvant.
4. 4. A fertilizer or fertilizer additive according to claim 3 wherein the viable count of Streptomyces sp. is not less than 10 6 CFU/mL.
5. 5. A biopesticide comprising the Streptomyces sp according to claim 1 and/or the microbial agent according to claim 2 and an acceptable adjuvant.
6. 6. Use of Streptomyces sp according to claim 1, a microbial agent according to claim 2, a fertilizer or fertilizer additive according to claim 3 or 4 and/or a biopesticide according to claim 5 for controlling banana vascular wilt.
7. 7. The use according to claim 6, wherein said banana vascular wilt is caused by fusarium oxysporum dedicated tropical physiological race No. 4.
8. 8. Use of Streptomyces sp according to claim 1, a microbial agent according to claim 2 and/or a fertilizer or fertilizer additive according to claim 3 or 4 for promoting plant growth.
9. 9. A method for controlling banana vascular wilt, characterized in that the Streptomyces sp according to claim 1, the microbial agent according to claim 2, the fertilizer or fertilizer additive according to claim 3 or 4 and/or the biopesticide according to claim 5 are applied in the growing environment of plants.
10. 10. The control method according to claim 9, wherein the Streptomyces sp is applied in an amount of 10 6 ~10 7 CFU/mL or The viable count of the Streptomyces sp in the microbial agent is 10 6 ~10 7 CFU/mL, or The number of viable bacteria of the Streptomyces sp in the fertilizer or fertilizer additive is 10 6 ~10 7 CFU/mL, or The number of viable bacteria of the Streptomyces sp. in the biopesticide is 10 6 ~10 7 CFU/mL.

Description

Streptomyces, microbial preparation and application thereof Technical Field The invention relates to the field of microorganisms, in particular to streptomyces, a microbial preparation and application thereof. Background Banana wilt, especially diseases caused by Fusarium oxysporum Guba specialization tropical physiological race 4 (Foc TR 4), is one of the most devastating threats facing the banana industry worldwide. The pathogenic bacteria infects banana vascular bundles, causes plant wilting and death, can survive in soil for a long time, and has limited effects and is not environment-friendly in the traditional chemical control and rotation method. The biological control by utilizing antagonistic microorganisms with plant rhizosphere growth promotion function is a very promising strategy in sustainable agriculture. However, the biocontrol strain reported in the prior art generally has the problems of narrow disease resistance spectrum, unstable growth promoting effect, weak colonization capability, poor effect in a complex field environment and the like. Therefore, the novel resources of the localized microorganisms with high-efficiency antagonism Foc TR4 and remarkable growth promoting function are separated and screened from the specific ecological niche of the banana rhizosphere, and the novel resources have important significance for developing high-efficiency and stable biological control products and realizing sustainable development of banana industry. Disclosure of Invention In view of this, the present invention provides Streptomyces, microbial preparations and uses thereof. The invention provides a novel streptomycete strain LE64, a preparation containing the strain and application thereof. The strain can effectively inhibit banana vascular wilt and greatly promote banana plant growth, and provides an environment-friendly microbial solution for solving the banana vascular wilt problem. In order to achieve the above object, the present invention provides the following technical solutions: The present invention provides Streptomyces sp, deposited under the accession number: GDMCC 67625. The invention also provides a microbial agent, which comprises any one of the following and acceptable auxiliary agents: (a) Streptomyces sp., as described above, and/or (B) The above-mentioned inactivated bacteria of Streptomyces sp, and/or (C) Metabolites, derivatives, fermentation broths, cultures, exosomes, lysates or extracts of the above Streptomyces sp. The invention also provides a fertilizer or a fertilizer additive, comprising the Streptomyces sp and/or the microbial agent and acceptable auxiliary agents. In some embodiments of the invention, in the above fertilizer or fertilizer additive, the viable count of Streptomyces sp. is not less than 10 6 CFU/mL. The invention also provides a biological pesticide which comprises the Streptomyces sp and/or the microbial agent and acceptable auxiliary agents. The invention also provides application of the Streptomyces sp, the microbial agent, the fertilizer or fertilizer additive and/or the biological pesticide in preventing and treating banana vascular wilt. In some embodiments of the invention, the banana vascular wilt is caused by fusarium oxysporum gulf-specialised tropical physiological race No. 4 in the above-mentioned applications. The invention also provides application of the Streptomyces sp, the microbial agent and/or the fertilizer or fertilizer additive in promoting plant growth. The invention also provides a method for preventing and treating banana vascular wilt, which comprises the step of applying the Streptomyces sp, the microbial agent, the fertilizer or fertilizer additive and/or the biological pesticide in a plant growth environment. In some embodiments of the present invention, in the above control method, the Streptomyces sp is applied in an amount of 10 6~107 CFU/mL, or The viable count of the Streptomyces sp in the microbial agent is 10 6~107 CFU/mL, or The number of viable bacteria of the Streptomyces sp in the fertilizer or fertilizer additive is 10 6~107 CFU/mL, or The number of viable bacteria of the Streptomyces sp. in the biopesticide is 10 6~107 CFU/mL. The beneficial effects of the invention include: (1) The invention provides a brand new microbial resource with definite taxonomy novelty and highly integrated functions. By heterogeneous taxonomic identification (16S rRNA gene sequence and whole genome ANI/dDDH analysis), this strain was identified as a new species of Streptomyces (Streptomyces) (example 1). In the prior art, the banana root system has strong antagonism Foc TR and comprehensive growth promoting functions, and is suitable for the lack of localized microorganism resources in banana root environment. The strain LE64 is obtained by directional screening from the specific ecological niche of the rhizosphere of healthy banana plants. (2) The strain provided by the invention realizes the synergistic effect of 'promoting gr