CN-121991855-A - Bacillus pseudomycoides BN59 and application thereof in promoting growth of wheat

Abstract

The invention belongs to the technical field of microbial fertilizers, and particularly relates to bacillus pseudomycosis BN59 and application thereof in promoting growth of wheat. A bacillus pseudomycoides BN59, wherein the preservation number of the bacillus pseudomycoides BN59 is CGMCC No.36454. The bacillus pseudomycosis BN59 provided by the invention has remarkable growth promoting effect on root length, plant height and fresh weight of wheat in seedling stage, has remarkable growth promoting effect on plant height and yield of potted wheat, and has a field growth promoting and yield increasing effect of 19.21%. And the BN59 is a natural microorganism, has higher safety to plants, environment, people and livestock, does not generate toxic and harmful substances in the fermentation and bacterial liquid preparation processes, and provides theoretical support and technical approach for development and production of the wheat microorganism bacterial fertilizer.

Inventors

• XIE LANFEN
• KAN YUNCHAO
• LIU RUNQIANG
• XU LI
• FEI LIWEI
• Liu Wuyu

Assignees

• 河南科技学院

Dates

Publication Date

20260508

Application Date

20260306

Claims (7)

1. 1. The bacillus pseudomycosis BN59 is characterized in that the preservation number of the bacillus pseudomycosis BN59 is CGMCC No.36454.
2. 2. Use of bacillus pseudomycosis BN59 according to claim 1 for promoting wheat growth.
3. 3. The method of claim 2, wherein promoting growth of wheat comprises promoting root length, shoot length, plant height, fresh weight, spike length, and spike number of wheat.
4. 4. A method for promoting wheat growth by using the bacillus pseudomycoides BN59 as defined in claim 1, which is characterized by comprising the step of treating wheat seeds by using the bacillus pseudomycoides BN59 fermentation liquid, wherein the viable count of the bacillus pseudomycoides BN59 is not lower than 1.0X10 7 CFU/mL.
5. 5. The method of claim 4, wherein the fermentation broth of bacillus pseudomycoides BN59 is diluted 10-100 fold.
6. 6. The method according to claim 4, wherein the wheat seeds are treated with the fermentation broth of Bacillus pseudomycoides BN59 by irrigation, seed soaking and/or seed dressing.
7. 7. A microbial fertilizer for wheat, comprising bacillus pseudomycosis BN59 or a fermentation broth thereof according to claim 1.

Description

Bacillus pseudomycoides BN59 and application thereof in promoting growth of wheat Technical Field The invention belongs to the technical field of microbial fertilizers, and particularly relates to bacillus pseudomycosis BN59 and application thereof in promoting growth of wheat. Background Wheat (Triticum aestivum l.) is an important staple food crop worldwide, a food source for 30% of the population. It supplies 8-20% protein and 20% energy to humans. For improving the yield of wheat, a chemical fertilizer application mode is mainly adopted for realizing the wheat yield improvement. The long-term use of chemical fertilizers in large quantities causes a series of production and environmental problems, such as reduced land productivity, heavy metal accumulation (mainly from chemical phosphate fertilizers), water eutrophication, reduced biodiversity, food safety and the like. At present, main measures of fertilizer reduction in agricultural production in China comprise soil testing and formulated fertilization, accurate fertilization according to soil nutrient supply and crop nutrient requirements, organic-inorganic coordination and other traditional technical means. Under the condition of continuous cropping high yield, the traditional technology is only used for reducing the application and enhancing the efficiency, so that a new thought needs to be created, a new technology is developed and a new product is developed, and the microbial technology is one of important means for activating soil nutrients, promoting plant growth, enhancing crop stress resistance, improving fertilizer efficiency and reducing the application of fertilizer. The plant rhizosphere growth promoting bacteria (plant growth promoting rhizobacteria, PGPR) have various physiological and ecological functions, and are safe, nontoxic, resource-saving and environment-friendly. Currently, there are a few tens of thousands of PGPR bacterial fertilizers registered in China, and the active bacterial components mainly comprise bacillus subtilis, paenibacillus mucilaginosus, bacillus megaterium and other groups. However, the number of PGPR bacterial manure which is registered and applied to wheat is only 500, and the phenomena of poor effect, high cost and the like exist. Therefore, the breeding of more excellent wheat PGPR strain is urgently needed, so as to provide reliable microbial strain resources for the yield increase and the application reduction of wheat planting industry. Disclosure of Invention The invention aims to provide application of bacillus pseudomycosis BN59 in promoting growth of wheat, so as to solve the problems in the prior art in the background art. In order to achieve the above purpose, the present invention provides the following technical solutions: the first object of the present invention is to provide a strain of Bacillus pseudomycosis BN59, wherein the preservation number of the Bacillus pseudomycosis BN59 is CGMCC No.36454. The second object of the invention is to provide the application of the bacillus pseudomycoides BN59 in promoting wheat growth. Further, the wheat growth promotion comprises promotion of root length, bud length, plant height, fresh weight, spike length and spike grain number of wheat. The third object of the invention is to provide a method for promoting wheat growth by using the bacillus pseudomycosis BN59, which comprises the step of adopting the bacillus pseudomycosis BN59 fermentation liquid to treat wheat seeds, wherein the viable count of the bacillus pseudomycosis BN59 is not lower than 1.0 multiplied by 10 7 CFU/mL. Further, the dilution factor of the fermentation liquid of the bacillus pseudomycoides BN59 is 10-100 times. Further, the wheat seeds are treated by the bacillus pseudomycoides BN59 fermentation liquid in a watering treatment, a seed soaking treatment and/or a seed dressing coating treatment. The fourth object of the present invention is to provide a wheat microbial fertilizer comprising the bacillus pseudomycoides BN59 or a fermentation broth thereof. Compared with the prior art, the invention has the following beneficial effects: the bacillus pseudomycosis BN59 provided by the invention has obvious growth promoting effect on root length, plant height and fresh weight of seedling wheat, has obvious growth promoting effect on plant height and yield of potted wheat, has a field growth promoting and yield increasing effect of 19.21%, and can effectively promote growth and yield increasing of the growth promoting wheat. Meanwhile, BN59 is a natural microorganism derived from wheat rhizosphere soil, has higher safety to plants, environment, people and livestock, and does not generate toxic and harmful substances in the fermentation and bacterial liquid preparation processes. Description of biological preservation Bacillus pseudomycosis (Baclllus pseudomycoides) BN59 is preserved in China general microbiological culture Collection center (CGMCC) at 12 and 31 days 2025, and has an ad