CN-121991856-A - Lactobacillus paracasei CCFM1529 capable of promoting bifidobacterium bifidum growth and freeze-drying survival through metabolism

Abstract

The invention discloses a bifidobacterium bifidum growth promoting and freeze-drying surviving lactobacillus paracasei CCFM1529 by metabolism, belonging to the field of microbial fermentation engineering. The method is characterized in that bifidobacterium bifidum is inoculated into the fermentation supernatant of the lactobacillus paracasei for culture, so that the maximum biomass of the bifidobacterium bifidum CCFM1167 is increased by 115.17 percent, and the freeze-drying survival rate is increased by 20.18 percent.

Inventors

• CUI SHUMAO
• SHEN TIANYU
• MAO BINGYONG
• TANG XIN
• ZHANG QIUXIANG
• LIU FEI
• ZHAI QIXIAO
• ZHAO JIANXIN
• CHEN WEI

Assignees

• 江南大学

Dates

Publication Date

20260508

Application Date

20260311

Claims (10)

1. 1. A strain of Lactobacillus paracasei (Lacticaseibacillus paracasei) CCFM1529, said Lactobacillus paracasei CCFM1529 having been deposited with the Guangdong province microorganism strain collection under the accession number GDMCC No:67539.
2. 2. The metagen prepared by using the lactobacillus paracasei CCFM1529 according to claim 1, wherein the metagen is obtained by fermenting the lactobacillus paracasei CCFM1529 and collecting the fermentation supernatant.
3. 3. The metazoan according to claim 2, wherein the fermentation is carried out at a temperature of 35-39 ℃ and is carried out at a temperature of 20-30 h.
4. 4. The metagen according to claim 3, further comprising glucose at a final concentration of 20 to 30 g/L, wherein the metagen has a pH of 6.0 to 6.1.
5. 5. The metazoan according to claim 4, wherein the fermentation medium comprises 5-10 g/L beef extract, 5-10 g/L peptone, 5-10 g/L yeast extract, 20-30 g/L glucose, 2-5 g/L sodium acetate, 2-5 g/L diammonium hydrogen citrate 、2-5 g/L K 2 HPO 4 ·3H 2 O、0-0.5 g/L MgSO 4 ·7H 2 O、0-0.5 g/L MnSO 4 ·H 2 O、1-2 mL/L Tween 80、1-2 g/L cysteine, and water.
6. 6. A method for promoting the growth of bifidobacterium bifidum, which is characterized in that bifidobacterium bifidum is inoculated into the metazoan of any one of claims 2-5 for fermentation.
7. 7. The method of claim 6, wherein the bifidobacterium bifidum comprises bifidobacterium bifidum CCFM1167.
8. 8. The method according to claim 7, wherein the fermentation is performed under anaerobic conditions of 35-39 ℃.
9. 9. A method for promoting freeze-drying survival of bifidobacterium bifidum is characterized by inoculating bifidobacterium bifidum into the metazoan of any one of claims 2-5 for fermentation, and collecting thalli for freeze-drying after fermentation, wherein the bifidobacterium bifidum comprises bifidobacterium bifidum CCFM1167.
10. 10. The method according to claim 9, wherein after the collection of the cells, the lyoprotectant solution is mixed with the cells in a ratio of (2:1) - (4:1) v/m, wherein the lyoprotectant solution comprises 30-50 g/L trehalose, 30-50 g/L sucrose, and 100-200 g/L skim milk powder.

Description

Lactobacillus paracasei CCFM1529 capable of promoting bifidobacterium bifidum growth and freeze-drying survival through metabolism Technical Field The invention relates to a bifidobacterium bifidum growth promoting and freeze-drying surviving lactobacillus paracasei CCFM1529 by metabolism, belonging to the field of microbial fermentation engineering. Background The bifidobacterium bifidum is used as one of the core probiotics of the human intestinal canal, plays a key role in early colonisation of the infant intestinal canal and healthy microecology construction, can efficiently degrade various dietary fibers to produce short chain fatty acids, regulate the pH value of the intestinal canal, and can reduce the occurrence risk of allergic and inflammatory bowel diseases through the interaction of unique surface molecules and a host immune system. In addition, the application value of the strain specificity in the fields of metabolic syndrome, mental health and the like is becoming a research hotspot, and the important significance of the strain specificity in deep development is highlighted. However, the industrial high-density cultivation of bifidobacteria bifidus faces significant biological limitations, as a strictly anaerobic heterotrophic microorganism, the growth of which is severely dependent on complex nutrient sources and is extremely sensitive to the redox potential of the culture medium, and the growth of which can be irreversibly inhibited by a small amount of oxygen. In the high-density fermentation process, the bacteria can be rapidly metabolized to produce organic acid, so that the osmotic pressure of a culture system is rapidly increased, and the bifidobacterium bifidum is sensitive to osmotic pressure change due to relatively weak thallus structure, so that the final biomass is severely limited. Meanwhile, the protein lacks complete anabolic pathway and has dependence on growth factors such as various amino acids, vitamins, nucleotides and the like. These complex nutritional requirements and stringent anaerobic environmental controls make it difficult to optimize by conventional processes for achieving economically viable industrialized high-density cultures. Subsequent freeze-drying processes are also a serious challenge for the maintenance of bifidobacterium bifidum activity. The physical and chemical stress such as ice crystal formation, osmotic pressure rapid change, membrane lipid phase change and the like in the freeze-drying process can directly damage the integrity of cell walls and cell membranes of the thalli, so that intracellular substances of the thalli leak, and finally the bioactivity is lost. Although freeze-drying protective agents such as trehalose, skim milk and the like are widely applied, for environmental sensitive strains such as bifidobacterium bifidum, the conventional protective agent compounding optimization often has limited protective efficiency, so that the survival rate and stability of live bacteria after freeze-drying are greatly attenuated. Therefore, development of a biotechnological method which can optimize the fermentation process to obtain high biomass and effectively enhance the freeze-drying stress resistance of the plant without depending on complex equipment has become an urgent need for breaking through the preparation bottleneck and improving the competitiveness of the plant. Bifidobacterium bifidum CCFM1167 is a disclosed functional strain with constipation relieving effect, and in practical industrial application, the problems of slow strain growth and low freeze-drying survival rate are faced. How to improve the growth efficiency of bifidobacterium bifidum CCFM1167 and the freeze-drying survival rate is a technical problem which needs to be solved in the prior art. Disclosure of Invention In order to solve the problems in the prior art, the invention provides the application of the Lactobacillus paracasei CCFM1529 and the fermentation supernatant thereof in promoting the growth and freeze-drying survival of bifidobacterium bifidum. The invention provides a strain of cheese bacillus paracasei (Lacticaseibacillus paracasei) CCFM1529, wherein the cheese bacillus paracasei CCFM1529 is preserved in the microorganism strain collection of Guangdong province at the 12 th month of 2025, the preservation number is GDMCC No:67539, and the preservation address is building 59 of No. 100 university in Guangzhou city martyr. In one embodiment, the lactobacillus paracasei CCFM1529 has the following characteristics: In one embodiment, the cells of the Lactobacillus paracasei CCFM1529 are slightly irregular, round-ended, non-motile Campylobacter under a microscope, and the colonies after 48h of culture on MRS solid medium are generally milky white, smooth convex, and round with a diameter of 1-2 mm. In one embodiment, the lactobacillus paracasei CCFM1529 is a gram positive bacterium, is facultative anaerobic, has a temperature preference, has an optimal growth te