CN-121991857-A - Streptomyces murinus FLWZ-0001 and application thereof in preventing and controlling crop fungi and oomycete diseases

Abstract

The application discloses Streptomyces murinus FLWZ-0001 and application thereof in preventing and controlling crop fungi and oomycete diseases. Streptomyces murinus (Streptomyces murinus) FLWZ-0001 is preserved in China Center for Type Culture Collection (CCTCC) with the preservation number of M20241923 at 9 and 5 of 2024. The strain and the fermentation liquor thereof have remarkable inhibition effect on various plant pathogenic bacteria, can inhibit pathogenic bacteria of fungus kingdom such as sclerotinia sclerotiorum, botrytis cinerea, penicillium digitatum and the like, and can inhibit phytophthora capsici and phytophthora nicotianae belonging to oomycetes. A potting experiment shows that the strain fermentation liquor has excellent control effect on a plurality of crop diseases such as sclerotinia rot of rape, black shank of rape, pepper epidemic disease, gray mold and the like. The application provides brand new microbial resources for developing broad-spectrum, efficient and environment-friendly multi-target biopesticide and has wide application prospect.

Inventors

• LIU FANG
• WU ZHAOYUAN
• WU HONGQU
• WANG YUEYING
• YE TING
• KE SHAOYONG
• WANG KAIMEI

Assignees

• 湖北省生物农药工程研究中心

Dates

Publication Date

20260508

Application Date

20260320

Claims (10)

1. 1. Streptomyces murinus which is preserved in China center for type culture collection (China center for type culture Collection) at 9 and 5 of 2024 is classified and named as Streptomyces murinus (Streptomyces murinus) FLWZ-0001, and has a preservation number of CCTCC NO: M20241923, and a place of China, university of Wuhan and Wuhan.
2. 2. The liquid fermentation broth of streptomyces murinus FLWZ-0001 of claim 1, prepared by a process comprising the steps of: (1) Seed liquid culture, namely inoculating streptomyces murinus FLWZ-0001 into a seed culture medium, and carrying out shake culture at 28 ℃ and 150 r/min for 4 days to obtain seed liquid; (2) Liquid fermentation, namely inoculating seed liquid into a fermentation culture medium according to 10 percent of inoculum size, and carrying out shaking culture for 7 days at 28 ℃ and 150 r/min to obtain fermentation liquid.
3. 3. Use of streptomyces murinus FLWZ-0001 according to claim 1 or of a liquid fermentation broth according to claim 2 for the preparation of a microbial preparation for controlling plant diseases.
4. 4. The use according to claim 3, wherein the plant disease is a disease caused by fungal pathogens and/or a disease caused by oomycete pathogens.
5. 5. The use according to claim 4, wherein the fungal pathogen comprises at least one of sclerotinia, botrytis cinerea, penicillium digitatum, penicillium italicum, alternaria colletotrichum, alternaria solani, fusarium graminearum, fusarium verticillium, phytophthora litchi, alternaria pyriformis and Mucor pulmonale, and the oomycete pathogen comprises at least one of Phytophthora capsici and Phytophthora nicotianae.
6. 6. The use according to claim 3, wherein the plant disease is at least one of sclerotium disease, black shank disease, pepper blight, gray mold, tobacco phytophthora disease, citrus green mold, fruit anthracnose, tomato early blight, wheat scab, corn ear rot, litchi downy mildew, pear black spot or leaf blight of tea.
7. 7. The use according to claim 6, wherein the plant disease is a combination of sclerotinia rot and black shank of rape or a combination of pepper epidemic and gray mold.
8. 8. The use according to claim 3, wherein the use is by spraying, rooting or dipping the liquid fermentation broth of claim 2 after dilution on plants, plant seeds, plant fruits or plant growth substrates.
9. 9. A method for controlling plant diseases, which comprises the step of applying the Streptomyces murinus FLWZ-0001 of claim 1 or the liquid fermentation broth of claim 2 to plants, plant seeds, plant fruits or plant growth substrates.
10. 10. Use of streptomyces murinus FLWZ-0001 according to claim 1 for screening active metabolites for controlling plant diseases.

Description

Streptomyces murinus FLWZ-0001 and application thereof in preventing and controlling crop fungi and oomycete diseases Technical Field The application relates to the technical field of biological control of plant diseases, in particular to Streptomyces murinus FLWZ-0001 and application thereof in controlling crop fungi and oomycete diseases. More specifically, it relates to a new strain FLWZ-0001 of Streptomyces murinus (Streptomyces murinus) with broad-spectrum efficient control effect on crop fungal diseases and oomycetes diseases, a fermentation liquor thereof and application thereof in controlling various plant diseases such as sclerotinia rot of rape, black shank of rape, pepper epidemic disease, gray mold, tobacco epidemic disease and citrus postharvest diseases. Background Rape and capsicum are important cash crops in China and occupy important roles in agricultural production. However, during its growth and development, it is often threatened by various pathogenic bacteria, resulting in serious yield loss and quality degradation. Wherein, sclerotinia sclerotiorum caused by sclerotinia sclerotiorum (Sclerotinia sclerotiorum) and rape black shank caused by black shank germ (Leptosphaeria maculans) are two major destructive diseases in rape production, which can lead to yield reduction and even harvest failure. Meanwhile, phytophthora capsici (Phytophthora capsici) and gray mold (Botrytis cinerea) are caused to the pepper industry, and the diseases are often mixed and have great control difficulty. At present, the prevention and treatment of the diseases mainly depend on chemical bactericides. However, the long-term use of a large amount of chemical pesticides not only easily causes the pathogenic bacteria to generate drug resistance and increases the prevention and treatment cost, but also causes the problems of exceeding pesticide residues, environmental pollution and the like, and does not meet the requirements of green and sustainable development of modern agriculture. Therefore, the development of an environment-friendly, safe and efficient biological control technology has become a research hotspot in the field of plant protection. The use of beneficial microorganisms and their metabolites to control plant diseases is one of the important directions of biological control. Presently, reported biocontrol bacteria mainly include Trichoderma (Trichoderma spp.), bacillus (Bacillus spp.), pseudomonas spp.), and the like. However, the existing biocontrol bacteria have the defects of narrow control spectrum, unstable control effect and the like. For example, trichoderma has a certain control effect on sclerotium disease but poor effect on black shank, and Bacillus can inhibit part of soil-borne diseases but has limited control effect on air-borne diseases (such as gray mold). So far, no report of a single microorganism strain capable of simultaneously and efficiently preventing and treating sclerotinia rot, black shank and pepper epidemic disease and gray mold has been found. Streptomyces spp is an important class of biocontrol actinomycetes capable of producing abundant secondary metabolites with broad-spectrum antibacterial activity. Among them, streptomyces murinus (Streptomyces murinus) has been studied as a species of this genus, and some strains thereof have been shown to have biocontrol potential. For example, chinese patent document CN104726362a discloses a strain of streptomyces murinus FXQ-5B isolated from mangrove soil, which has a remarkable antagonistic effect on camellia oleifera leaf blight bacteria (trichoderma microsporum Pestalotiopsis microspora) and a certain inhibitory effect on pathogenic bacteria of various fungal diseases such as fusarium oxysporum (Fusarium oxysporum). However, the strain and related researches are mainly focused on the prevention and treatment of oil tea diseases and partial fungal diseases, do not relate to the prevention and treatment effect on main diseases of crops such as rape, peppers and the like, particularly on Phytophthora spp (Phytophthora spp) which belongs to oomycetes (oomyceta) in taxonomy, and have limitation on application range. Therefore, the prior art is lack of a broad-spectrum biocontrol strain capable of simultaneously and efficiently controlling fungus diseases (such as sclerotium disease, black shank and gray mold) and oomycete diseases (such as epidemic disease) on various crops such as rape, capsicum and the like. Therefore, a new Streptomyces murinus strain which has broad-spectrum antibacterial activity, high control effect on fungi and oomycetes and strong environmental adaptability is developed, and has important theoretical and application values for developing multi-target biological pesticides and solving the actual disease problem in agricultural production. Disclosure of Invention Therefore, the application aims to provide a novel streptomyces griseofulvus strain with broad-spectrum efficient control effects on crop fungal dise