CN-121991859-A - Deep sea bacillus megatherium and application thereof

Abstract

The invention discloses a deep sea bacillus megatherium and application thereof, belonging to the technical field of agricultural microorganism and biological control. The invention obtains a deep sea bacillus megatherium strain NT-3-5 through screening and identification, and experiments prove that the deep sea bacillus megatherium strain NT-3-5 provided by the invention can resist salt and alkali and grow in an environment with 12% NaCl and pH 9, the strain NT-3-5 can obviously inhibit various plant fungus pathogenic bacteria and plant bacterial pathogenic bacteria, and the potting prevention effect of the strain NH-3-5 on pepper southern blight reaches 72.09%. Therefore, the deep sea bacillus megatherium strain NT-3-5 provided by the invention can be used for preparing products for inhibiting plant fungal pathogenic bacteria and plant bacterial pathogenic bacteria and preventing and controlling plant diseases, is beneficial to promoting agricultural sustainable development, can greatly reduce environmental pollution, and has good application prospects.

Inventors

• Shao Anqi
• SHAO MINGSHI
• LI BOWEN
• TIAN YANJU

Assignees

• 新乡市金谷生物技术有限公司

Dates

Publication Date

20260508

Application Date

20260325

Claims (10)

1. 1. A strain of deep sea bacillus megatherium (Oceanobacillus profundus) NH-3-5 is characterized in that the deep sea bacillus megatherium NH-3-5 is deposited at the microorganism strain collection of Guangdong province on the year 01 month 04 of 2026, the deposition number is GDMCC No:67589, and the deposition address is the institute of microorganisms of Guangdong province, seisakusho 100 No. 59 building 5 building, guangzhou City, martyr.
2. 2. The use of the deep sea bacillus megatherium NH-3-5 according to claim 1 for the preparation of a microbial agent.
3. 3. A microbial agent comprising the deep sea bacillus marinus NH-3-5 or a fermentation broth thereof of claim 1.
4. 4. Use of the deep sea bacillus megatherium NH-3-5 or its fermentation broth of claim 1 or the microbial agent of claim 3 for inhibiting plant fungal pathogens and plant bacterial pathogens.
5. 5. Use of the deep sea bacillus megatherium NH-3-5 or a fermentation broth thereof of claim 1 or the microbial agent of claim 3 for the preparation of a product for inhibiting plant fungal pathogens and plant bacterial pathogens.
6. 6. The use according to claim 4 or 5, wherein the plant fungal pathogens include southern blight (Sclerotium rolfsii), phytophthora capsici (Phytophthora capsici Leonian), sheath blight corn (Rhizoctonia solani) and/or colletotrichum capsici (Colletotrichum capsici); The plant bacterial pathogens include black rot of radish (Xanthomonas campestris pv. campescris), soft rot of konjak (Erwinia carotovora subsp. Carotovora), bacterial blight of rice (Ralstonia solanacearum) and/or bacterial wilt of tomato (Ralstonia solanacearum).
7. 7. Use of the deep sea bacillus megatherium NH-3-5 or its fermentation broth of claim 1 or the microbial inoculant of claim 3 for controlling plant diseases, wherein the plant diseases comprise capsicum southern blight.
8. 8. Use of the deep sea bacillus megatherium NH-3-5 or a fermentation broth thereof according to claim 1 or the microbial inoculum according to claim 3 for the preparation of a product for controlling plant diseases, wherein the plant diseases comprise capsicum southern blight.
9. 9. A method for inhibiting plant fungal pathogens and plant bacterial pathogens comprising the step of treating the plant fungal pathogens and plant bacterial pathogens with the fermentation broth of bacillus megatherium NH-3-5 of claim 1; The plant fungal pathogens comprise capsicum southern blight germ, phytophthora capsici leonian, rhizoctonia solani and/or capsicum anthracnose germ; the plant bacterial pathogens comprise black rot of radish, soft rot of konjak, bacterial leaf blight of rice and/or bacterial wilt of tomato.
10. 10. A method for controlling plant diseases, which comprises the step of irrigating plants with the fermentation broth of the deep sea bacillus marinus NH-3-5 of claim 1; The plant diseases include capsicum southern blight.

Description

Deep sea bacillus megatherium and application thereof Technical Field The invention relates to the technical field of agricultural microorganism and biological control, in particular to deep sea bacillus and application thereof. Background Soil salinization is a core adversity problem that restricts the sustainable development of global agriculture. As a typical extreme habitat, the high-salt ion poisoning and high-pH dual stress of the saline-alkali soil can directly destroy the microecological balance of plant rhizosphere, disturb the cell permeation balance and ion steady state, inhibit the absorption of root system nutrients and the normal growth and development of plants, thereby greatly reducing the crop yield, and simultaneously, the saline-alkali stress can reduce the disease resistance and stress resistance of the plants, exacerbate the occurrence and spread of soil facsimile diseases such as fusarium, saprophytium and the like, and form the dual hazard of 'saline-alkali stress + disease infection'. Compared with common cultivated lands, the method for developing and utilizing the saline-alkali soil in large scale has irreplaceable strategic significance, and not only can make a large amount of idle low-efficiency land resources be coiled, effectively enlarge the amount of the reserved cultivated lands, build the national grain safety barrier, but also can improve the ecology of regional soil, inhibit the further salinization and degradation of the land and realize win-win ecological benefit and economic benefit. And the special extreme environment of the saline-alkali soil also provides urgent demands for the green prevention and control of crop stress resistance and growth promotion and soil fax bacteria diseases far exceeding the common soil. The traditional chemical modifier and chemical pesticide are easy to lose efficacy and have great discounted effect in a high-salt and high-alkali environment, secondary problems such as secondary pollution to soil, drug resistance increase of pathogenic bacteria, unbalance of rhizosphere microbial communities and the like are caused, and the ecological improvement requirement of the saline-alkali soil is difficult to adapt, and the saline-alkali resistant functional microorganism-mediated biological improvement technology has the characteristics of being ecological, strong in targeting, good in sustainability and the like, can actively adapt to the saline-alkali extreme environment, directionally regulate and control rhizosphere micro-ecology, and becomes the core research and application direction of the current saline-alkali soil agricultural field. As an important plant rhizosphere growth promoting bacterium, bacillus has saline-alkali tolerance, bacteriostasis and growth promoting potential, can relieve plant salt stress through metabolic regulation, is suitable for extreme habitat of saline-alkali soil, can inhibit common soil fax bacteria pathogenic bacteria of the saline-alkali soil from secreting antibacterial substances in a targeted manner, can promote crop growth through nitrogen fixation, phosphorus dissolution, growth hormone production and other modes, and meets the special requirements of the saline-alkali soil planting industry. Therefore, the high-quality saline-alkali-resistant microorganism strain which has outstanding saline-alkali resistance, obvious antibacterial activity on the special soil facsimile bacteria pathogenic bacteria of the saline-alkali soil, stable promotion effect and strong rhizosphere colonization capacity is screened and obtained, and the application of the high-quality saline-alkali-resistant microorganism strain in the ecological improvement of the saline-alkali soil, the biological control of the crop soil facsimile bacteria diseases and the stress-resistant promotion yield increase of crops is developed, so that the gap of the functional microorganism resources of the current high-adaptability saline-alkali soil can be filled, the large-scale and green planting industry development of the saline-alkali soil can be promoted, and the high-quality saline-alkali-soil microbial strain has important practical significance and application value for realizing the efficient utilization of the resources of the saline-alkali soil, the development of ecological sustainable agriculture and the guarantee of grain safety, and is a key research direction to be broken through in the current agricultural microorganism and saline-alkali soil treatment field. Disclosure of Invention The invention aims to provide the deep sea bacillus megatherium strain NT-3-5 which can be used for preparing products for inhibiting plant fungal pathogenic bacteria and plant bacterial pathogenic bacteria and preventing plant diseases, is beneficial to promoting agricultural sustainable development, can greatly reduce environmental pollution, and has good application prospect. In order to achieve the above object, the present invention provides the fo