CN-121991860-A - Acid-resistant slow rooting tumor bacteria for degrading nitrous oxide and application thereof

Abstract

The invention belongs to the technical field of microorganisms, and particularly relates to acid-resistant slow rooting tumor bacteria for degrading nitrous oxide and application thereof. An acid-resistant slow-growing rhizobia which is a beet slow-growing rhizobia (Bradyrhizobium betae) purchased from China general microbiological culture Collection center, and has a preservation number of CGMCC 1.15594. The acid-resistant slow rooting tumor bacteria can still keep good growth state and physiological activity under the condition of low pH value and no additional carbon source assistance. After the fertilizer is applied to farmlands, stable colonization can be realized, the pH value of soil can be effectively buffered and improved through own metabolic activity, the rhizosphere microenvironment of crops is improved, meanwhile, the biological holding capacity of a soil microbial community on nitrogen is enhanced, inorganic nitrogen is trapped, and a biological foundation is provided for preventing and controlling agricultural non-point source pollution, reducing nitrogen fertilizer loss and fertilizer application reduction and efficiency improvement.

Inventors

• WANG ZITING
• Zhou Shengsen
• SUN YONGFENG

Assignees

• 广西大学

Dates

Publication Date

20260508

Application Date

20260327

Claims (8)

1. 1. The acid-resistant slow-growing rhizobia is characterized by being a beet slow-growing rhizobia (Bradyrhizobium betae) purchased from China general microbiological culture collection center, and the collection number is CGMCC 1.15594.
2. 2. Use of the bradyrhizobium of claim 1 for degrading nitrous oxide.
3. 3. Use of the bradyrhizobium of claim 1 for improving the acidic environment of soil.
4. 4. Use of a bradyrhizobium according to claim 1 for inhibiting nitrifying functional microorganisms.
5. 5. Use of the bradyrhizobium of claim 1 for reducing the carbon to nitrogen ratio of a soil microorganism.
6. 6. Use of the slow-growing rhizobia of claim 1 in the content of acid-insoluble organic nitrogen in soil.
7. 7. A microbial agent comprising the acid-fast slow rooting tumor of claim 1.
8. 8. The microbial agent according to claim 7, wherein the preparation method of the microbial agent comprises the following steps: S1, culturing the acid-resistant slow rooting tumor strain in a YMA solid culture medium at a constant temperature of 28 ℃ until full single colonies grow; S2, selecting the single colony after activation, transferring to YMA liquid culture medium, and carrying out shake culture at 28 ℃ and 150-200rpm until the single colony reaches a logarithmic growth phase to obtain fermentation bacteria liquid; S3, collecting effective thalli in the fermentation broth, and re-suspending and adjusting the concentration of the broth to 1X 10 8 -1×10¹ 0 CFU/mL to obtain the microbial inoculum.

Description

Acid-resistant slow rooting tumor bacteria for degrading nitrous oxide and application thereof Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to acid-resistant slow rooting tumor bacteria for degrading nitrous oxide and application thereof. Background Nitrous oxide (N 2 O) is an important greenhouse gas with a global warming potential far exceeding carbon dioxide. Farmland soil is the main source of artificial N 2 O emissions. Acidic red soil is widely distributed in tropical and subtropical areas such as south China. Because nitrogen fertilizer is excessively applied throughout the year in intensive agricultural production (such as cash crops like sugarcane, etc.), serious surplus nitrogen and low utilization rate are caused, and the acidification process of soil is further accelerated. Soil acidification not only restricts crop growth, but also greatly promotes the emission of N 2 O as a direct negative result, so that the area becomes the key point and the difficulty of emission reduction of agricultural greenhouse gases. N 2 O reductase encoded by nosZ gene in microorganisms is the only enzyme known to reduce N 2 O to harmless nitrogen (N 2) in the biosphere. However, prior studies have generally shown that low pH significantly inhibits the final reduction process of N 2 O. The biochemical mechanism is mainly that the lower pH value does not necessarily directly inhibit nosZ transcription, but can seriously interfere the posttranscriptional assembly and functional expression of N 2 O reductase in the periplasm of cells, so that a large amount of N 2 O generated in acid soil cannot be thoroughly reduced and discharged into the atmosphere. Although weak N 2 O reduction was observed in some acid soils by the scientific community, so far few functional microorganisms capable of independent growth under acidic conditions and possessing a high N 2 O reduction rate were isolated. Some pure culture strains (such as Rhodanobacter) reported in the prior literature have reducing capability at pH3.7-4.0, but can not grow basically at the acidity, while other co-cultures (such as Serratia sp. And Desulfosporosinus sp.) have the effect of reducing N 2 O at pH 4.5, and not only are auxiliary carbon sources such as pyruvic acid and the like needed, but also the time for completely removing N 2 O is long, so that the carbon reduction requirement of practical farmlands is difficult to meet. Therefore, the screening of functional strains which can tolerate low pH, independently grow and effectively reduce N 2 O from typical acidic farmland soil is of great significance in preventing and controlling the emission of N 2 O in agriculture. The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. Disclosure of Invention The invention aims to provide an acid-resistant slow rooting tumor bacterium for degrading nitrous oxide and application thereof, so as to solve the problems in the prior art in the background technology. In order to achieve the above purpose, the present invention provides the following technical solutions: An acid-resistant slow-growing rhizobia which is a beet slow-growing rhizobia (Bradyrhizobium betae) purchased from China general microbiological culture Collection center, and has a preservation number of CGMCC 1.15594. The invention also provides application of the slow-growing rhizobia in degrading nitrous oxide, improving the acidic environment of soil, inhibiting nitrifying function microbial bacteria, reducing the carbon-nitrogen ratio of soil microorganisms, or improving the content of acid-insoluble organic nitrogen in soil. The invention also provides a microbial agent, which comprises the acid-resistant slow rooting tumor bacteria. Further, the preparation method of the microbial agent comprises the following steps: S1, culturing the acid-resistant slow rooting tumor strain in a YMA solid culture medium at a constant temperature of 28 ℃ until full single colonies grow; S2, selecting the single colony after activation, transferring to YMA liquid culture medium, and carrying out shake culture at 28 ℃ and 150-200rpm until the single colony reaches a logarithmic growth phase to obtain fermentation bacteria liquid; S3, collecting effective thalli in the fermentation broth, and re-suspending and adjusting the concentration of the broth to 1X 10 8-1×10¹0 CFU/mL to obtain the microbial inoculum. Compared with the prior art, the invention has the following beneficial effects: (1) The acid-resistant slow rooting tumor bacteria can still keep good growth state and physiological activity under the condition of low pH value and no additional carbon source assistance. After the fertilizer is applied to farmla