CN-121991861-A - Nourishing arthrobacter strain and application thereof

Abstract

The invention provides a nourishing arthrobacter strain named nourishing arthrobacter Arthrobacter pascens, which is preserved in the China general microbiological culture Collection center (CGMCC) No.37526, and the preservation date is 2026, 01 and 26 days, and the 16S rRNA nucleotide sequence of the nourishing arthrobacter Arthrobacter pascens is shown as SEQ ID NO. 1. Also provided are uses for promoting plant growth and improving plant quality. The arthrobacter lyticum Arthrobacter pascens has the capabilities of potassium decomposition, IAA secretion and siderophore production, can promote plant growth and improve plant quality, and is used for promoting the growth of new wheat seedlings.

Inventors

• YUN LAN
• NARENTUYA
• JIA ZHENYU
• LI ZHEN
• AI QIAN
• DAI RUI
• ZHOU CHENGMEI
• LIU QIYU

Assignees

• 内蒙古农业大学

Dates

Publication Date

20260508

Application Date

20260328

Claims (9)

1. 1. A nourishing Arthrobacter strain is named as nourishing Arthrobacter Arthrobacter pascens, and is preserved in China general microbiological culture Collection center (CGMCC) No.37526 and has a preservation date of 2026 and 26 days of 01 month, and a 16S rRNA nucleotide sequence of Arthrobacter pascens is shown as SEQ ID NO. 1.
2. 2. Use of a trophoblast strain according to claim 1, wherein the trophoblast strain is used to promote plant growth and improve plant quality.
3. 3. The use according to claim 2, characterized in that the plant is neo-wheat straw.
4. 4. Use according to claim 3, characterized in that the trophoblast strain is used to promote the growth of young wheat seedlings.
5. 5. The use according to claim 4, wherein the Arthrobacter trophoblasts strain is used to promote plant height, root length, fresh weight above ground and fresh weight below ground of young wheat straw seedlings.
6. 6. The use according to claim 2, characterized in that the trophoblast strain is prepared as a bacterial suspension.
7. 7. The use according to claim 6, wherein the bacterial suspension is prepared by the following method: The arthrobacter trophae Arthrobacter pascens is inoculated in an LB liquid culture medium, shake-cultured for 48 hours at the temperature of 25 ℃ and the rotating speed of 180rpm, and the concentration is adjusted to OD 600 =0.8, so as to obtain a bacterial suspension.
8. 8. The use according to claim 7, wherein the method of application of the bacterial suspension is root irrigation.
9. 9. The use of the arthrobacter trophoblasts strain of claim 1, wherein the arthrobacter trophoblasts Arthrobacter pascen has potassium-releasing, IAA-secreting and siderophore-producing capabilities.

Description

Nourishing arthrobacter strain and application thereof Technical Field The invention belongs to the technical field of Arthrobacter, and particularly relates to a nourishing Arthrobacter strain and application thereof. Background Wheat grass [ Psathyrostachys juncea (Fisch.) Nevski ] is also called Russian wild rye (Russian wildrye), is a perennial cross-pollinated plant of Gramineae, is native to Siberian and China and is distributed in Xinjiang of China, is excellent perennial pasture, and is widely cultivated in northern grassland areas of China, north American plains, russian and the like. The new wheat straw has strong stress resistance, developed root system, drought resistance, cold resistance, grazing resistance, barren resistance and salt and alkali resistance, long annual nutrition growth period, high nutrition value and strong regeneration, and is an ideal grass seed for establishing artificial grasslands and grazing grasslands. Has great potential in ecological restoration of degraded grasslands in northwest arid, semiarid and alpine regions of China. Is suitable for developing grassland animal husbandry in north China, improving natural grasslands and establishing artificial grasslands, and is also an excellent rural grassland seed for ecological construction in cold and dry areas in north China. Many students have studied new wheat plants from many aspects such as biology, physiology, breeding and cultivation, and particularly have attempted to analyze the stress resistance of the wheat plants from a genetic aspect. However, there is still a lack of research on root-soil interfaces of the plants directly related to stress resistance. Plant roots are the first barrier to stress and stress adaptation, and more studies indicate that the interaction between plant roots and specific microorganisms is specific, and thus rhizosphere microorganisms are also called "second genomes" of plants. The continuous growth and accumulation of root systems of perennial plants tend to form stable interaction relationship with microorganisms, so that the soil microenvironment and the plant growth and development are influenced for a long time. Grass seeds are preferred for ecological construction of the degenerated grasslands in the arid regions, and often need to survive and grow under poor environmental conditions, and the outstanding stress resistance is closely related to the action exerted by rhizosphere microorganisms, especially the growth promotion action of microorganisms in the seedling planting period. The slow growth of new wheat straw in seedling stage and the negative effect of environmental stress, how to establish the beneficial and stable root system-microbe interaction relationship as soon as possible are key jamming points for the early success of the grass planting. Therefore, the method screens specific pro-biotic microorganisms and utilizes the pro-biotic microorganisms to help the successful establishment of perennial grass seedlings, thereby having important value for ecological construction engineering and artificial grassland construction in cold and dry areas of China. Plant rhizosphere growth promoting bacteria (PGPR) act as a beneficial bacteria that colonizes around the plant root system, and can act directly or indirectly to promote plant growth. How to find key rhizosphere growth promoting bacteria and utilize the growth promoting effect of the rhizosphere growth promoting bacteria to promote the growth of new wheat straw in seedling stage is a key technical problem to be solved urgently at present. The present study collected and identified 33 rhizosphere bacteria from the rhizosphere of new wheat grass grown for many years at a number of different sites, from which strains with stronger physiological activity and significant growth promoting effect on new wheat grass seedlings were screened. So as to provide technical support for the efficient cultivation and management of new wheat straw. Disclosure of Invention Aiming at the defects of the prior art, the invention provides a nourishing arthrobacter strain and application thereof, wherein the nourishing arthrobacter Arthrobacter pascens has the capabilities of potassium dissolving, IAA secretion and siderophore production, can promote plant growth and plant quality, and is used for promoting the growth of new wheat straw seedlings. In order to solve the technical problems, the invention adopts the technical scheme that the Arthrobacter trophoblast strain is named as Arthrobacter trophoblast Arthrobacter pascens, is preserved in China general microbiological culture Collection center (China general microbiological culture Collection center), has a preservation address of North Star Xway No.1, china academy of sciences microbiological study, has a preservation number of CGMCC No.37526, has a preservation date of 2026, 01 and 26 days, and has a 16S rRNA nucleotide sequence of Arthrobacter pascens shown as SEQ ID NO. 1. The invention