CN-121991865-A - Staphylococcus vortioides and composite microbial inoculum thereof, preparation method and application

Abstract

A staphylococcus vortioides and a composite microbial agent, a preparation method and application thereof belong to the technical field of microorganisms, the staphylococcus vortioides (Staphylococcus warneri) has a strain name of LH-403 and a preservation number of CGMCC No.36214, and the staphylococcus vortioides and the trichoderma longibrachiatum (Trichoderma longibrachiatum) and the bacillus thuringiensis (Bacillus thuringiensis) LMU-81 are jointly prepared to obtain the composite microbial agent. The invention combines the three strains to generate synergistic effect, can produce a large amount of lignocellulose at normal temperature, and is suitable for degrading bagasse lignocellulose.

Inventors

• ZHOU HAO
• HUANG ZHI
• Nong Chengtao
• CHEN SIMIN
• LI JIANBIN
• ZHU BIYANG
• LI KAI
• He Huiban
• Xie Lianglong
• LIANG HANZHU
• DING DE
• DENG LIGAO

Assignees

• 广西大学

Dates

Publication Date

20260508

Application Date

20260410

Claims (6)

1. 1. A staphylococcus vortioides is characterized in that the staphylococcus vortioides is named as staphylococcus vortioides (Staphylococcus warneri) in a classification mode, the staphylococcus vortioides is named as LH-403 in a preservation number of CGMCC No.36214, the preservation number of the staphylococcus vortioides is China general microbiological culture Collection center, and the preservation date is 2025, 10 months and 9 days.
2. 2. Use of staphylococcus vortioides as defined in claim 1 for the preparation of a complex bacterial agent promoting lignocellulose degradation.
3. 3. The use of Staphylococcus vortioides according to claim 1 for the preparation of a biological preparation for producing cellulases and ligninases, wherein the cellulases comprise one or more of exo-glucanase Exg, endo-glucanase Eng, beta-glucosidase beta-glu and filter paper enzyme FPA, and the ligninases comprise one or more of laccase Lac, lignin peroxidase LiP and manganese peroxidase MnP.
4. 4. A composite microbial inoculum is characterized in that the composite microbial inoculum is prepared from staphylococcus vortioides (Staphylococcus warneri) LH-403, trichoderma longibrachiatum (Trichoderma longibrachiatum) and bacillus thuringiensis (Bacillus thuringiensis) LMU-81; The staphylococcus vortioides (Staphylococcus warneri) LH-403 is preserved in China general microbiological culture Collection center (CGMCC) with a preservation number of CGMCC No.36214 at the 10 th month 9 of 2025; The trichoderma longibrachiatum (Trichoderma longibrachiatum) is preserved in China center for type culture Collection of microorganisms, and the preservation number is CICC 41185; the Bacillus thuringiensis (Bacillus thuringiensis) LMU-81 is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 36215 in the year 10 and the day 9 of 2025.
5. 5. The method for preparing the composite microbial inoculum according to claim 4, wherein the method comprises the steps of inoculating staphylococcus vortioides (Staphylococcus warneri) LH-403, trichoderma longibrachiatum (Trichoderma longibrachiatum) and bacillus thuringiensis (Bacillus thuringiensis) LMU-81 into a liquid culture medium respectively, culturing at 28 ℃ and adjusting the colony number to 1X 10 8 CFU/mL, and mixing the strains in equal volumes to obtain the composite microbial inoculum.
6. 6. The use of the composite microbial inoculant of claim 4 for degrading sugarcane sugar-making waste.

Description

Staphylococcus vortioides and composite microbial inoculum thereof, preparation method and application Technical Field The invention belongs to the technical field of microorganisms, and particularly relates to staphylococcus vortioides and a composite microbial agent, a preparation method and application thereof. Background The degradation period of the natural lignocellulose under natural conditions is longer, and the utilization rate is low. Therefore, the microbial method with low cost, environmental friendliness and convenient operation is selected to degrade the natural lignocellulose, and the microbial method is widely paid attention to people. However, the use of a single microorganism for lignocellulose degradation has limitations such as long culture time and low efficiency. In order to achieve efficient biodegradation, this can be achieved by complex microorganisms consisting of different strains. The synergistic effect between different functional microorganisms is used as the key of efficient degradation, and the composite microbial system shows stronger decomposing capability and stable lignocellulose activity compared with any single strain. Because of higher substrate utilization rate, the compound microorganism gradually replaces a single strain, and becomes the first choice method for biodegrading lignocellulose. The composite microbial community is discussed to have great significance for degradation of lignocellulose. At present, the constructed compound microorganism is mainly obtained through targeted domestication and artificial construction. The composite microorganism can be subjected to targeted domestication, so that the function of the composite microorganism can be remarkably improved, but the time required for domestication is long. The artificial construction of the compound microbiome has the advantages of promoting the complementary advantages among microorganisms and integrally improving the performance of the compound microorganisms. The artificial construction does not simply mechanically bring the microorganisms together for physical mixing, but rather requires the adjustment and promotion of the synergy between them, further advancing the biochemical reaction process. Balancing the production requirements of different microorganisms is therefore a key to promote their co-operation. In the process of selecting the strain, the cross-boundary combination of the fungi and bacteria is compounded, so that the cross-boundary combination of the fungi and bacteria has more unexpected effect than the cross-boundary combination of the fungi and the fungi. The method is characterized in that competition between bacteria and fungi is avoided to prevent efficiency from being reduced, competition for resources and space are not easy to occur, the stability of the whole degradation system depends on the living state of the fungi, once the environment is uncomfortable, the whole degradation process can be rapidly stopped, the fungi provide living physical supports and degraded nutrient sources for the bacteria, and the bacteria quickly consume small molecular products to create favorable conditions for continuous enzymolysis activities of the fungi. Therefore, the different effects on lignocellulose degradation by adopting different bacteria and fungus composite microbial inoculum combinations are the continuous exploration direction of the technicians in the field. Staphylococcus vortioides (Staphylococcus warneri), belonging to the genus Staphylococcus, is widely present in the normal flora of human skin. The strain has a diameter of 0.5-1.5 mu m, can form single-strain, double-strain or grape-shaped arrangement, and can resist a high-salt environment. The method is mainly applied to research and development of pathogen detection chips, classification research and fermentation food development. In previous studies, most of the lignocellulose enzymes were derived from fungi, in particular from the genera trichoderma and aspergillus. There is a very recent report in the prior art on the ability of staphylococcus vortioides to be used for the breakdown of lignocellulose. For example, china patent refers to a staphylococcus vortioides AACE8 capable of promoting growth of armillaria mellea and application thereof, publication number refers to CN119639609A, and the staphylococcus vortioides AACE8 has cellulose degradation capability but specific degradation capability is unknown. In the prior art, although a plurality of lignocellulose degrading composite microbial agents exist, the composite microbial agents can only realize the effect of efficiently degrading one or two components of cellulose, hemicellulose and lignin, the degradation capability is not balanced, and the degradation capability of the existing composite microbial agents is unsatisfactory when the existing composite microbial agents are applied to substrates with different sources and different component ratios. Disclosure