CN-121991878-A - Tissue culture and rapid propagation culture medium for poplar and tissue culture method thereof

Abstract

The invention discloses a poplar tissue culture rapid propagation culture medium and a tissue culture method thereof, belonging to the technical field of plant tissue culture. The invention firstly uses the fullerol (fullerene modified with 24 hydroxyl groups) in the field of plant tissue culture, and prepares the poplar tissue culture rapid propagation culture medium by adding the fullerol, the ABT rooting powder and the plant hormone into a 1/2MS culture medium. Based on the culture medium, the invention also provides a poplar tissue culture method. Experimental results show that the rooting culture is carried out by using the poplar tissue culture rapid propagation culture medium provided by the invention, the root system development of different varieties of poplar tissue culture seedlings can be obviously promoted, the rooting rate of adventitious roots is improved, and the surface area and length of the root system are increased. The invention provides a novel culture medium and a tissue culture method for effectively solving the problems that the rooting culture stage of tissue culture and rapid propagation of poplar is long in period, the root system is thin and weak, vitrification is easy, the death is easy in a greenhouse seedling hardening link and the like, and provides technical support for efficient seedling culture of poplar.

Inventors

• CUI ZHIMIN
• MA XIAOXIA
• SHE PING
• LI BAOLONG
• GUAN YUANYUAN
• FENG LICHENG

Assignees

• 山西省桑干河杨树丰产林实验局科技服务中心

Dates

Publication Date

20260508

Application Date

20260211

Claims (10)

1. 1. The poplar tissue culture rapid propagation culture medium is characterized by comprising a basic culture medium, fullerol and ABT rooting powder.
2. 2. The poplar tissue culture rapid propagation medium of claim 1 wherein the minimal medium is a 1/2MS medium.
3. 3. The poplar tissue culture rapid propagation medium according to claim 1, wherein the concentration of the fullerol is 1-6mg/L.
4. 4. The poplar tissue culture rapid propagation medium of claim 1 wherein the concentration of ABT rooting powder is 0.5mg/L.
5. 5. The poplar tissue culture rapid propagation medium of claim 1 further comprising 0.05mg/L NAA, 0.3mg/L IBA, 5g/L agar powder and 15g/L sucrose.
6. 6. Use of the poplar tissue culture rapid propagation medium according to any one of claims 1 to 5 in poplar tissue culture.
7. 7. The use according to claim 6, wherein the poplar is aspen-pie poplar.
8. 8. The use according to claim 6, wherein the variety of poplar is 84K poplar, populus tomentosa or populus tomentosa.
9. 9. A poplar tissue culture method, which is characterized by comprising the step of inoculating a poplar tissue culture seedling to the poplar tissue culture rapid propagation medium according to any one of claims 1 to 5 for rooting culture.
10. 10. The tissue culture method of poplar of claim 9 wherein the variety of poplar is 84K poplar, populus tomentosa or populus tomentosa.

Description

Tissue culture and rapid propagation culture medium for poplar and tissue culture method thereof Technical Field The invention relates to the technical field of plant tissue culture, in particular to a poplar tissue culture rapid propagation culture medium and a tissue culture method thereof. Background The tissue culture technology is a asexual propagation technology developed on the basis of totipotency of plant cells, and is mainly realized by aseptic operation, so that a large amount of complete plants are obtained in a short time under the condition of manual control. The tissue culture technology has the advantages of being capable of accurately controlling the environment, free of season limitation, high in propagation speed, high in propagation efficiency, material-saving, convenient to manage, capable of guaranteeing regular and consistent propagation offspring, keeping excellent characters of original varieties and the like, becomes an effective way for rapid propagation of the forest, and particularly has great application value for the forest with long growth cycle, difficult sexual propagation and low cutting survival rate. At present, tree species such as Eucalyptus (Eucalyptus) and hawthorn (Crataegus pinnatifida) have been subjected to in vitro rapid propagation by using a tissue culture technology. Poplar (Populus) is one of important tree species widely cultivated in the world, has the excellent characteristics of high and upright tree body, rapid growth in seedling stage, good wood quality, strong adaptability, strong disease and insect resistance and the like, and is an excellent tree species for building material forests and protecting forests. In recent years, as the task of poplar forestation is intensified, the requirement of using tissue culture rapid propagation technology to carry out poplar seedling raising is increased. However, the tissue culture propagation process is complex, on one hand, the concentration and the component ratio of the hormone preparation are not easy to control, and in the tissue culture process of poplar, the hormone types and the concentrations suitable for adventitious bud regeneration have great differences due to different poplar varieties. On the other hand, in tissue culture of poplar plants, a plurality of varieties belong to tree species difficult to root, a relatively good tissue culture method is needed to obtain relatively high rooting rate and robust plants, the tissue culture and rapid propagation rooting culture stage of poplar pie poplar is relatively long in period, the root system of the tissue culture seedling is thin and weak and is easy to vitrify, and the seedling is easy to die in a greenhouse seedling hardening link. These factors limit the development and application of tissue culture and rapid propagation technology of poplar, so it is highly desirable to provide a tissue culture method suitable for various poplar tissue culture and rapid propagation culture medium and shortening culture period. Disclosure of Invention The invention aims to provide a poplar tissue culture rapid propagation culture medium and a tissue culture method thereof, so as to solve the problems of the prior art, and the invention provides a new culture medium and a tissue culture method for effectively solving the problems that the period of rooting culture stage of Yang Shuzu culture rapid propagation is long, the root system is thin and weak, vitrification is easy, the death is easy in a greenhouse seedling hardening link and the like by adding fullerol (fullerene modified with 24 hydroxyl groups) into a 1/2MS culture medium to prepare the poplar tissue culture rapid propagation culture medium. In order to achieve the above object, the present invention provides the following solutions: the invention provides a poplar tissue culture rapid propagation culture medium which comprises a basic culture medium, fullerol and ABT rooting powder. Further, the minimal medium is 1/2MS medium. Further, the concentration of the fullerol is 1-6mg/L. Further, the concentration of the ABT rooting powder is 0.5mg/L. Further, the feed additive also comprises 0.05mg/L NAA, 0.3mg/L IBA, 5g/L agar powder and 15g/L sucrose. The invention provides an application of the poplar tissue culture rapid propagation culture medium in poplar tissue culture. Further, the poplar is a aspen pie poplar. Further, the variety of poplar is 84K poplar, white poplar or castanea longifolia. The invention provides a poplar tissue culture method, which comprises the step of inoculating poplar tissue culture seedlings to the poplar tissue culture rapid propagation culture medium for rooting culture. Further, the variety of poplar is 84K poplar, white poplar or castanea longifolia. The invention discloses the following technical effects: The invention firstly uses the fullerol (fullerene modified with 24 hydroxyl groups) in the field of plant tissue culture, and prepares the poplar tissue culture rapid