CN-121991887-A - Culture medium for improving synthesis of unsaturated fatty acid in cell culture fat

Abstract

The invention relates to the technical field of biological culture, in particular to a culture medium for improving the synthesis of unsaturated fatty acid in cell culture fat, which is used for solving the problem that the content of the unsaturated fatty acid in the culture fat is not satisfactory. Among them, specific induction factors include, but are not limited to, insulin, dexamethasone, indomethacin, 3-isobutyl-1-methylxanthine, rosiglitazone, and the like. The unsaturated fatty acid additive, including alpha-linolenic acid, can raise the differentiation efficiency of pig adipose mesenchymal stem cell and the directional synthesis of intracellular unsaturated fatty acid through adding these matters into culture medium. The improved differentiation culture medium realizes effective promotion of cell differentiation and unsaturated fatty acid synthesis by optimizing components and proportion, and has important application prospect.

Inventors

• SONG WENJUAN
• WAN JIAHUI
• DONG YUNXIANG
• LI XINRAN
• ZHAO ZONGXIN
• LIANG ZHUO
• GUO XUEQIANG
• FAN ZHENLIN
• CHANG XINJIAN
• WANG WEIYUN
• SHEN YAPING
• XI LINGLING
• She Songbo

Assignees

• 新乡医学院

Dates

Publication Date

20260508

Application Date

20241226

Claims (6)

1. 1. A culture medium for improving the synthesis of unsaturated fatty acid in cell culture fat is characterized by comprising the following components of an induction factor for promoting the adipogenic differentiation of stem cells, an exogenous unsaturated fatty acid additive and the balance of a basic culture medium containing 10% of fetal calf serum and 1% of penicillin and streptomycin double antibody.
2. 2. The medium of claim 1, wherein the induction factor comprises one or more of insulin, dexamethasone, indomethacin, 3-isobutyl-1-methylxanthine, rosiglitazone.
3. 3. The medium for increasing the synthesis of unsaturated fatty acids in cell culture fat according to claim 1, wherein the unsaturated fatty acid additive comprises alpha-linolenic acid.
4. 4. The medium for increasing the synthesis of unsaturated fatty acids in cell culture fat according to claim 1, wherein the basal medium comprises one of DMEM high sugar medium, DMEM low sugar medium, MEM medium, DMEM/F12 medium, and F10 medium.
5. 5. The medium for increasing the synthesis of unsaturated fatty acids in cell culture fat according to claim 2, wherein the induction factor comprises one or more of 5-50 μg/mL insulin, 1-10 μΜ dexamethasone, 0.1-10 mm indomethacin, 0.1-10 mm 3-isobutyl-1-methylxanthine, 1-10 μΜ rosiglitazone.
6. 6. A culture medium for increasing the synthesis of unsaturated fatty acids in cell culture fat according to claim 3, wherein the unsaturated fatty acid additive is added in an amount of 10 μm to 10mM of alpha-linolenic acid.

Description

Culture medium for improving synthesis of unsaturated fatty acid in cell culture fat Technical Field The invention relates to the technical field of biological culture, in particular to a culture medium for improving the synthesis of unsaturated fatty acid in cell culture fat. Background Fat determines the flavor, texture, nutrition and visual appearance of meat and also has a significant impact on the juiciness and mouthfeel of meat, and thus cell culture fat plays a vital role in cell culture of meat. In the process of producing cell culture fat, improving the differentiation efficiency of fat cells and the content of unsaturated fatty acid are of great significance for improving the quality of cultured meat. Unsaturated fatty acids (Unsaturated FATTY ACI DS, UFA), such as n-3 and n-6 fatty acids, are critical to human health and play an important role in preventing cardiovascular disease, anti-inflammatory and promoting brain development, etc. However, conventional media often fail to effectively promote adipocyte differentiation and unsaturated fatty acid synthesis. The currently accepted medium formulation for inducing the differentiation of adipose-derived mesenchymal stem cells (ad ipose t i ssue-DER IVED MESENCHYMA L STEM CE L L, ADSCs) to mature adipocytes is DMEM/F12 basal medium supplemented with 10% fetal bovine serum and 1% diabody and combined induction with five inducers of 3-isobutyl-1-methylxanthine, insulin, dexamethasone, indomethacin and rosiglitazone. The culture medium can achieve better differentiation effect about 10 days of differentiation. However, the use of this differentiation medium to induce ADSCs differentiation efficiency and unsaturated fatty acid content in the cultured fat is not satisfactory. Alpha-linolenic acid is an easily available n-3PUFA with wide sources, is a necessary fatty acid for the body, and can be used for synthesizing other types of n-3 PUFAs, such as EPA and DHA. Research shows that the fatty acid composition in the livestock and poultry bodies is directly influenced by the fatty acid composition in daily ration, and the addition amount and the continuous addition time of PUFA in the daily ration can regulate and control the synthesis and catabolism of the PUFA in the livestock and poultry bodies, so that the deposition amount of the PUFA in the livestock and poultry products is determined. The linseed is rich in alpha-linolenic acid, and researches show that the addition of the linseed in the daily ration of fattening pigs can obviously improve the UFA content in pork. Based on the above, a culture medium for improving the synthesis of unsaturated fatty acid in cell culture fat is provided, so that the defects of the conventional differentiation culture medium can be overcome. Disclosure of Invention The invention aims to provide a culture medium for improving the synthesis of unsaturated fatty acid in cell culture fat, which solves the problem that the unsaturated fatty acid content in the culture fat is unsatisfactory in the prior art. In order to achieve the above purpose, the present invention provides the following technical solutions: a culture medium for increasing the synthesis of unsaturated fatty acid in cell culture fat contains the induction factor for promoting the adipogenic differentiation of stem cells, exogenous unsaturated fatty acid additive and basic culture medium containing 10% of fetal calf serum and 1% of penicillin and streptomycin. Based on the technical scheme, the invention also provides the following optional technical schemes: in one alternative, the induction factor comprises one or more of insulin, dexamethasone, indomethacin, 3-isobutyl-1-methylxanthine, and rosiglitazone. In one alternative, the unsaturated fatty acid additive comprises alpha-linolenic acid. In an alternative, the basal medium comprises one of DMEM high-sugar medium, DMEM low-sugar medium, MEM medium, DMEM/F12 medium, F10 medium; In an alternative scheme, the induction factor comprises one or more of 5-50 mu g/mL insulin, 1-10 mu M dexamethasone, 0.1-10 mM indomethacin, 0.1-10 mM 3-isobutyl-1-methylxanthine and 1-10 mu M rosiglitazone. In an alternative, the unsaturated fatty acid additive is added as 10. Mu.M to 10mM alpha-linolenic acid. Compared with the prior art, the invention has the following beneficial effects: The invention can improve the differentiation efficiency of ADSCs and the directional synthesis of unsaturated fatty acid in cells. The improved differentiation culture medium realizes effective promotion of cell differentiation and unsaturated fatty acid synthesis by optimizing components and proportion, and has important application prospect. Drawings FIG. 1 shows the effect of alpha-linolenic acid on adipogenic differentiation of ADSCs (A: oil red O staining. B: quantitative analysis of adipogenic differentiation. C: expression level of adipogenic differentiation-related gene. P < 0.001). FIG. 2 is a chart of PCA analysis of the intrac