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CN-121991890-A - Stem cell-derived engineering nano vesicle for promoting hair generation and preparation method thereof

CN121991890ACN 121991890 ACN121991890 ACN 121991890ACN-121991890-A

Abstract

The invention relates to stem cell-derived engineering nano vesicles (e-NVs) for promoting hair generation, which are prepared from stem cells sequentially through engineering induction, apoptosis induction and procedural serial extrusion processes, wherein an inducer used for engineering induction is 3,3' -diindolylmethane. The particle size of the engineering nanometer vesicle is mainly distributed in 50-300 nm, the polydispersity index is 0.05-0.20, and the expression proportion of phosphatidylserine is 40% -50%. The rice vesicle can effectively improve the microenvironment of hair follicles and regulate the hair growth cycle, promote proliferation and differentiation of hair follicle stem cells, remarkably improve the hair regeneration rate of an androgenetic alopecia model mouse, and provide a safe, convenient and efficient clinical strategy for hair regeneration treatment.

Inventors

  • HUO MEIRONG
  • LIAN YUNFEI
  • LIU NING
  • GUO HAO

Assignees

  • 南京赛立康生物医学科技有限公司

Dates

Publication Date
20260508
Application Date
20260121

Claims (10)

  1. 1. The stem cell-derived engineering nano vesicle for promoting hair generation is characterized in that the engineering nano vesicle is prepared from stem cells sequentially through engineering induction, apoptosis induction and programmed serial extrusion processes, and an inducer used for the engineering induction is 3,3' -diindolylmethane.
  2. 2. The stem cell-derived engineering nanovesicle of claim 1, wherein the induction of apoptosis is chemical induction, physical method induction, or genetic control induction.
  3. 3. The stem cell derived engineering nanovesicle of claim 1, wherein the extrusion pressure of the tandem extrusion process is at 0.210.0mpa.
  4. 4. The stem cell-derived engineering nanovesicle of claim 1, wherein the tandem extrusion process is a sequential passage through a large pore porous membrane and a small pore porous membrane.
  5. 5. The stem cell derived engineering nanovesicle of claim 1, wherein the average particle size of the engineering nanovesicle is 100500nm.
  6. 6. The stem cell derived engineered nanocapsule of claim 1 wherein said engineered nanocapsule has a polydispersity index of 0.050.30.
  7. 7. The stem cell derived engineering nanovesicle of claim 1, wherein the surface of the engineering nanovesicle has a phosphatidylserine expression rate of greater than 35%.
  8. 8. The method for preparing stem cell-derived engineered nanovesicles as claimed in any one of claims 1 to 7, wherein the method comprises the steps of: (1) Culturing stem cells by using a culture medium, and when the cell growth confluence is 70-90%, engineering the stem cells by using a 3,3' -diindolylmethane solution to obtain engineering MSCs; (2) Discarding the culture medium supernatant of the engineering MSCs obtained in the step (1), adding a serum-free culture medium, performing apoptosis induction, and continuing to culture after the induction is finished; (3) Collecting the apoptosis-induced engineering MSCs obtained in the step (2), and carrying out programmed serial extrusion on the suspension containing the apoptosis-induced engineering MSCs by using serial extrusion equipment to obtain the apoptosis-induced engineering MSCs.
  9. 9. Use of the stem cell-derived engineered nanovesicles of any one of claims 1-7 in the manufacture of a medicament for preventing or treating hair loss or promoting hair regrowth.
  10. 10. The use according to claim 9, wherein the hair loss is androgenic hair loss, telogen loss, scarring hair loss, traction hair loss, lichen planus or alopecia areata.

Description

Stem cell-derived engineering nano vesicle for promoting hair generation and preparation method thereof Technical Field The invention belongs to the technical field of biology, and particularly relates to stem cell source engineering nano vesicles capable of promoting hair generation and a preparation method thereof. Background Alopecia is a worldwide common disease characterized by hair loss, wherein androgenetic alopecia (Androgenetic Alopecia, AGA) is the most common type of non-scarring alopecia, which not only severely affects the appearance and image of a patient, but also has a significant negative impact on mental health and social functions thereof, and has become an increasingly prominent public health problem. The current main means for clinically treating androgenic alopecia include drug therapy, surgical treatment and physical therapy, but these methods still have obvious limitations. External medicines represented by minoxidil need to be continuously used every day, and adverse reactions such as scalp itching and hirsutism are easily caused, and partial patients have insufficient response. Finasteride can reduce the level of Dihydrotestosterone (DHT) in hair follicles by inhibiting type II 5α -reductase, but may cause systemic adverse reactions such as sexual dysfunction, mood swings, etc., and hair is easy to shed again after drug withdrawal. The low-intensity laser treatment needs long-term regular irradiation, the action mechanism is not completely clear, and the curative effect is also obviously different from individual. Autologous hair transplantation, although improving local density, belongs to invasive surgery, is limited by donor area resources, is costly, and cannot improve the sustained degeneration of the primary hair follicle. In summary, the existing treatment schemes still have difficulty in meeting clinical demands in terms of safety, effectiveness and long-term maintenance, and development of a novel treatment strategy that is safe, efficient and well-adapted to patients is highly desirable. In recent years, stem cells and exosomes thereof have shown important potential in hair regeneration research, and have become a research hotspot in the field of biological therapy. The stem cell exosome can regulate hair follicle microenvironment through paracrine action due to carrying various bioactive molecules of parent cells, and provides a brand new thought for hair regeneration. However, the conventional stem cell exosomes still have key bottlenecks in the application of hair regeneration, such as low content of factors related to the exosome hair regeneration promotion and insufficient targeting, so that the efficiency of activating the proliferation and differentiation of the hair follicle stem cells is limited, and meanwhile, the exosome preparation process has the problems of low yield, difficult precise quality control and the like, restricts the mass production and clinical transformation process, and cannot fully meet the actual requirements of hair regeneration treatment. Therefore, the directional engineering modification of the stem cell vesicles and the optimization of the preparation process become the core direction for improving the hair regeneration efficiency and promoting the clinical application. Based on the method, an engineering stem cell nano vesicle (ENGINEERED NANOVESICLES, e-NVs) which is rich in key factors for promoting hair regeneration, efficient and controllable in preparation process and excellent in biological activity is developed and becomes a key break for solving the defects of the prior art. Disclosure of Invention In view of the problems of limited hair regeneration treatment means, low content of key regulation and control signal molecules for hair regeneration of vesicles from stem cells, uneven particle size distribution and the like in the prior art, the invention aims to provide stem cell-derived engineering nano vesicles for promoting hair generation and a preparation method thereof, so as to realize enrichment of the key regulation and control signal molecules for hair regeneration by vesicles and high-efficiency regeneration promotion in a hair follicle microenvironment, thereby overcoming the defects of the existing treatment scheme. In order to solve the technical problems, the invention provides stem cell-derived engineering nanovesicles (ENGINEERED NANOVESICLES, e-NVs) for promoting hair generation. The e-NVs realize the engineering of mesenchymal stem cells through the induction of DIM (3, 3'-diindolylmethane, 3' -Diindolylmethane), enrich more key regulation and control signal molecules for hair regeneration, and have remarkable hair regeneration promotion effect in the hair follicle microenvironment. Meanwhile, the induced mesenchymal stem cells adopt a serial extrusion process, so that the prepared e-NVs have more uniform particle size distribution. The first aspect of the invention provides stem cell-derived engineering nano v