CN-121991928-A - Insect lysozyme capable of preventing and treating rice virus diseases and application thereof

Abstract

The invention discloses insect lysozyme capable of preventing and treating rice virus diseases and application thereof. The invention innovatively digs three lysozyme (RdLYZI, rdLYZC1 and RdLYZC) with remarkable inhibitory activity on various rice viruses from the natural transmission vector insects of the viruses, and the amino acid sequences of the lysozyme are shown as SEQ ID NO.13, SEQ ID NO.14 and SEQ ID NO. 15. Experiments prove that the lysozyme RdLYZI, rdLYZC1 and RdLYZC can effectively inhibit the replication and accumulation of various rice viruses in plants and medium insects, have obvious prevention and treatment effects on related virus diseases and relieve plant growth disorder caused by the viruses. The invention provides a novel biological control strategy with environmental protection and strong targeting for green prevention and control of rice virus diseases, and has important application prospect.

Inventors

• WEI TAIYUN
• DU YU
• CHEN QIAN
• LI YOU

Assignees

• 福建农林大学

Dates

Publication Date

20260508

Application Date

20260224

Claims (10)

1. 1. An insect lysozyme is characterized by comprising an amino acid sequence shown in any one of the following: (1) Lysozyme RdLYZI1 with the amino acid sequence shown in SEQ ID NO. 13; (2) Lysozyme RdLYZI2 with the amino acid sequence shown in SEQ ID NO. 14; (3) Lysozyme RdLYZC1 with the amino acid sequence shown in SEQ ID NO. 15; (4) The amino acid sequence of lysozyme RdLYZC is shown as SEQ ID NO. 16.
2. 2. A nucleic acid molecule for encoding the insect lysozyme according to claim 1, wherein the nucleotide sequence of the nucleic acid molecule is shown in SEQ ID NO. 1-4.
3. 3. An expression cassette, a recombinant vector, a recombinant cell and a recombinant microorganism comprising the nucleic acid molecule of claim 2.
4. 4. A product for preventing and treating rice virus diseases is characterized in that the active ingredient is the insect lysozyme according to claim 1, or the nucleic acid molecule according to claim 2, or the expression cassette, the recombinant vector, the recombinant cell and the recombinant microorganism according to claim 3.
5. 5. The product of claim 4, wherein the rice virus disease comprises rice tumor dwarf, rice streak mosaic disease and southern rice black streak dwarf disease.
6. 6. The product of claim 4, wherein the product is effective to inhibit replication and accumulation of rice viruses in rice plants and virus-mediated insects and to restore rice plant growth disorders induced by rice viruses.
7. 7. The product of claim 6, wherein the vector insect is an electro-optic leafhopper or a sogatella furcifera.
8. 8. Use of insect lysozyme according to claim 1, a nucleic acid molecule according to claim 2 or an expression cassette for a nucleic acid molecule according to claim 3, a recombinant vector, a recombinant cell and a recombinant microorganism for the preparation of any one of the following products: (1) A product resistant to rice viruses; (2) Products for preventing and treating rice virus diseases; (3) A product that inhibits replication of rice viruses in its vector insect.
9. 9. The method of claim 8, wherein the rice virus disease comprises rice tumor dwarf, rice streak mosaic and southern rice black streak dwarf, and the vector insect is an electro-optic leafhopper or a sogatella furcifera.
10. 10. The method according to claim 8, wherein the product is applied in a manner of soaking a diseased rice root system or soaking seedlings before transplanting, and the concentration of the product is 0.5-4 mg/mL.

Description

Insect lysozyme capable of preventing and treating rice virus diseases and application thereof Technical Field The invention belongs to the field of plant protection and biotechnology, and in particular relates to insect lysozyme capable of preventing and treating rice virus diseases and application thereof. Background Rice is one of the most important grain crops worldwide, and the yield and quality of the rice directly affect the grain safety. However, various rice virus diseases seriously threaten rice production, and among them, diseases caused by southern rice black streaked dwarf virus (southern rice black-STREAKED DWARF virus, SRBSDV), rice tumor dwarf virus (RICE GALL DWARF virus, RGDV), rice streak mosaic virus (RICE STRIPE mosaic virus, RSMV) and the like are particularly prominent. The viruses are mainly transmitted through vector insects such as the plant hopper (Sogatella furcifera), the electro-optic leafhopper (Recilia dorsalis) and the like in a lasting proliferation mode, so that symptoms such as dwarfing of infected plants, thickening of leaf deformity, tumor-like protrusion, spike delay, obvious reduction of fruiting rate and the like are caused, and even the plants die when serious symptoms are caused, so that serious yield loss is caused. At present, the field control for the virus diseases mainly depends on chemical pesticides to kill virus-transmitting medium insects, and breeding and popularizing virus-resistant rice varieties. However, the long-term use of chemical pesticides in large quantities is liable to cause problems such as insect resistance, pesticide residues and environmental pollution. The breeding period of disease-resistant varieties is long, the cost is high, and the resistance of the disease-resistant varieties is possibly lost due to virus variation. Therefore, development of novel biocontrol agents that are environmentally friendly, target specific and not prone to induce resistance is particularly urgent. Lysozyme is a natural hydrolase widely existing in living bodies, is known for its broad-spectrum antibacterial activity, and has been used in the fields of medical treatment and food preservation. In recent years, their potential antiviral activity has also begun to be of interest. However, current research on lysozyme has focused on vertebrate sources (such as egg white lysozyme), and research on lysozyme from insect sources, especially its application in plant virus disease control, has remained blank. Disclosure of Invention Aiming at the limitations of the existing rice virus disease prevention and control means, the invention aims at identifying and developing a novel lysozyme with high-efficiency antiviral activity from the inside of insect-mediated electro-optic leafhoppers of rice viruses, and providing a novel active ingredient and solution for green prevention and control of rice virus diseases. In order to achieve the above purpose, the present invention adopts the following technical scheme: in a first aspect, the invention provides an insect lysozyme having an amino acid sequence as set forth in any one of the following: (1) Lysozyme RdLYZI1 with the amino acid sequence shown in SEQ ID NO. 13; (2) Lysozyme RdLYZI2 with the amino acid sequence shown in SEQ ID NO. 14; (3) Lysozyme RdLYZC1 with the amino acid sequence shown in SEQ ID NO. 15; (4) The amino acid sequence of lysozyme RdLYZC is shown as SEQ ID NO. 16. In a second aspect, the invention provides a nucleic acid molecule for encoding the insect lysozyme, and the nucleotide sequence of the nucleic acid molecule is shown in SEQ ID NO. 1-4 in sequence. In a third aspect, the invention provides expression cassettes, recombinant vectors, recombinant cells and recombinant microorganisms comprising the above-described nucleic acid molecules. In a fourth aspect, the invention provides a product for preventing and treating rice virus diseases, the active ingredients of which are the insect lysozyme or the nucleic acid molecule or the expression cassette, the recombinant vector, the recombinant cell and the recombinant microorganism. Further, the rice virus diseases include rice tumor dwarf, rice streak mosaic disease and southern rice black streak dwarf. Furthermore, the product can effectively inhibit the replication and accumulation of rice viruses in rice plants and virus-mediated insects, and recover rice plant growth disorder caused by the rice viruses. Further, the vector insect is an electro-optic leafhopper or a sogatella furcifera. In a fifth aspect, the present invention provides the use of an insect lysozyme as described above, a nucleic acid molecule as described above or an expression cassette, a recombinant vector, a recombinant cell and a recombinant microorganism as described above for the preparation of any one of the following products: (1) A product resistant to rice viruses; (2) Products for preventing and treating rice virus diseases; (3) A product that inhibits replication of rice