CN-121991946-A - Application of SlSWEET b gene in maintaining pollen viability of tomatoes under weak light stress

Abstract

The invention belongs to the technical field of plant genetic engineering, and discloses application of SlSWEET b gene in maintaining pollen viability of tomatoes under weak light stress. Members Solyc g071400 of tomato SWEET family have key regulation and control functions on carbohydrate metabolism and transportation in pollen development process under weak light stress. Specifically, functional deletions of the gene (such as sw5b-18, sw5b-22 and sw5b-24 mutants) significantly reduce pollen viability, indicating that it is essential in maintaining normal physiological function of pollen. This finding not only reveals a new function of SlSWEET family members in tomato reproductive development, but also provides an important clue for resolving the molecular mechanism of crop fertility decline under weak light stress.

Inventors

• LAN ZHIXIN
• YANG RONGCHAO
• ZHANG YUEQIN
• LI JIALONG
• SUN XIAOJIANG

Assignees

• 广东海洋大学

Dates

Publication Date

20260508

Application Date

20260306

Claims (3)

1. 1. The target knockout Solyc g071400 primer pair is characterized in that the primer pair is primer pair 1 and primer pair 2, the sequence of the primer pair 1 is shown as SEQ ID NO. 1-2, and the sequence of the primer pair 2 is shown as SEQ ID NO. 3-4.
2. Use of solac 06g071400 for maintaining pollen viability under low light stress in tomato.
3. Application of Solyc06g071400 in developing and/or screening products for improving pollen viability under weak light stress of tomato.

Description

Application of SlSWEET b gene in maintaining pollen viability of tomatoes under weak light stress Technical Field The invention belongs to the technical field of plant genetic engineering, and particularly relates to an application of SlSWEET b gene in maintaining pollen viability of tomatoes under weak light stress. Background Tomato (Solanum lycopersicum l.) belongs to an annual herb of the genus solanum of the family solanaceae, is native in south america, is widely cultivated worldwide, becomes an important vegetable crop worldwide, and occupies an important place in agricultural production in China. Tomato contains rich lycopene, vitamin C, carotene and the like, has extremely high nutritive value, has remarkable antioxidation effect on human body, and plays an important role in maintaining human health. According to statistics, in 2021, the tomato yield in China accounts for 1/3 of the tomato yield in the world, and the tomato facility cultivation position is the first in the facility cultivation area. However, tomatoes are regarded as a light-like crop, and a weak light environment is inevitably encountered in the facility cultivation process, so that the tomatoes are in a weak light state, the growth and development of the tomatoes are affected, and finally flower and fruit dropping and tomato yield reduction are caused. Tomato (Solanum lycopersicum l.) plants are very sensitive to weak light at all stages of development, especially one important external factor affecting inflorescence abscission at early stages of seedling growth is light. Plants have the ability to shed their vegetative and reproductive organs based on developmental signals and environmental stimuli. While this shedding process has many adaptive benefits, in an agricultural setting, premature shedding can result in significant decreases in crop productivity, such as fruit bearing failure. Shedding is a highly regulated process involving structural, biochemical and molecular changes, and the tissues of the shed organ are called shedding bands (AZ). These changes include protein degradation, cell wall remodeling, lamellar lysis, loss of cell membrane permeability, and programmed cell death. Weak light stress is the most important factor restricting plant growth and crop yield in winter and spring, and they induce oxidative stress by inhibiting photosynthesis, causing various metabolic changes. Under weak light stress, tomatoes are firstly stricken with chloroplast swelling, serious chloroplast membrane damage, light and system damage, ROS accumulation and oxidation resistance system damage in the plant growth and development process. Further affecting the growth and development of the tomato inflorescence, and the reproductive organs acquire reduced nutrients, at this time, the tomato plants shed off the reproductive organs according to the development signals and environmental stimulus. The cells in the shedding zone undergo a series of changes, such as protein degradation, cell wall remodeling, middle lamellar dissolution, loss of cell membrane permeability, programmed cell death and the like, and finally cause premature shedding of tomato flower organs, thereby seriously affecting the yield of tomatoes. At present, the research on the weak light stress of tomatoes at home and abroad does not have the influence focused on the development process of anthers, so the research aims at exploring the phenomenon that the plants purposefully fall flowers due to the fact that nutrients of tomatoes cannot meet the development of anthers under the weak light stress, and has important theoretical and application values for promoting the cultivation of weak light resistant varieties in tomatoes and other plants. Disclosure of Invention Based on the above-mentioned drawbacks of the prior art, the present invention first provides a primer pair for targeted knockout Solyc g 071400. The invention also provides an application of Solyc g071400 in maintaining pollen viability of tomatoes under weak light stress. The invention also provides an application of Solyc g071400 in developing and/or screening products for improving pollen viability under weak light stress of tomatoes. The aim of the invention is achieved by the following technical scheme: The invention discovers that the transcriptional level of the SlSWEET gene family member Solyc g071400 (ncbi: IPR 018179) is obviously different through transcriptome data, and the expression level of the transcriptome data is far higher than that of other genes (the expression level of a control group is 2946, and the expression level of a weak light group reaches 4453) in the period of mature pollen. From this we speculate that it plays an important role in pollen development. We therefore obtained transgenic plants by CRISPR/Cas9 technology and three mutants (sw 5b-18, sw5b-22, sw5 b-24). The mutants sw5b-18 (52 bp deletion), sw5b-24 (182 bp deletion) and sw5b-22 (55 bp deletion) cause serious disruption