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CN-121991953-A - Nucleic acid interferon for black chest termite and its application in preventing and controlling termite

CN121991953ACN 121991953 ACN121991953 ACN 121991953ACN-121991953-A

Abstract

The invention discloses a nucleic acid interferon of black-chest termite and application thereof in termite control, wherein the design of the three kinds of interferon is respectively derived from Catalase genes, medium CHAIN ACYL CoA ligase genes and C47753 genes in the black-chest termite body, and the DNA sequences are SEQ 1A, SEQ B and SEQ 1C respectively. Dsrnas of CAT, ACSF2 and C47753, respectively, i.e. SEQ 3A, SEQ B and SEQ 3C, i.e. the interferons of the three genes, were synthesized by the T7 in vitro transcription system. The CAT, ACSF2 and c47753 gene interferon obtained by the invention can weaken the physiological defensive ability of termites, obviously improve the control effect of metarhizium anisopliae on termites, and provide a novel targeting gene for environment-friendly termite control.

Inventors

  • LIU LONG
  • TANG QINGBO
  • FENG YIYING
  • HE FANGCHAN
  • Tang Gujin
  • Lv Zhengxun
  • ZHAO PENGFEI

Assignees

  • 河南农业大学

Dates

Publication Date
20260508
Application Date
20260106

Claims (10)

  1. 1. The nucleic acid interferon for the black-chest termites is characterized by comprising three genes, namely a key gene derived from the antioxidant function of the black-chest termites in vivo, a key enzyme gene derived from the synthesis of acyl-coa in the black-chest termites in vivo and a key gene derived from a transcriptome database of the black-chest termites.
  2. 2. A black-chest termite nucleic acid interferon according to claim 1 wherein: The key gene of the antioxidant function in the black-chest termite body, namely the Catalase (CAT) gene, the DNA sequence of which is derived from a black-chest termite transcriptome database and the DNA sequence of which is SEQ 1A; the key enzyme gene for catalyzing the synthesis of acyl-CoA in the body of the termite body, namely a Medium CHAIN ACYL CoA ligase (ACSF 2) gene, the DNA sequence of the gene is derived from a termite transcriptome database of the termite body, and the DNA sequence of the gene is SEQ 1B; the key gene from the black-chest termite transcriptome database, namely the C47753 gene, has the DNA sequence of SEQ 1C.
  3. 3. The nucleic acid interferon for Alternaria melanogaster as claimed in claim 1, wherein the CAT gene SEQ 1A of Alternaria melanogaster is CAT dsRNA template with the DNA sequence of SEQ 2A.
  4. 4. The nucleic acid interferon for Alternaria melanogaster of claim 1, wherein the ACSF2 gene SEQ 1B of the Alternaria melanogaster is designed into an ACSF2 dsRNA template, and the DNA sequence is SEQ 2B.
  5. 5. The nucleic acid interferon for Alternaria melanogaster of claim 1, wherein the termite C47753 gene SEQ 1C designs a C47753 dsRNA template, and the DNA sequence is SEQ 2C.
  6. 6. The nucleic acid interferon of Alternaria melanogaster of claim 3, wherein the CAT dsRNA template is prepared into CAT dsRNA by a T7 in vitro transcription system, and the RNA sequence is SEQ 3A.
  7. 7. The nucleic acid interferon for Alternaria melanogaster of claim 4, wherein the ACSF2dsRNA template is prepared into ACSF2dsRNA by a T7 in vitro transcription system, and the RNA sequence is SEQ 3B.
  8. 8. The nucleic acid interferon for Alternaria melanogaster of claim 5, wherein the C47753 dsRNA template generates C47753 dsRNA by a T7 in vitro transcription system, and the RNA sequence is SEQ 3C.
  9. 9. The use of nucleic acid interferon of black-chest termite according to any one of claims 1 to 8, wherein for termite control, namely, 3 nucleic acid interferon termite CAT dsRNA, ACSF2 dsRNA and c47753 dsRNA, the synergistic agent of the nucleic acid interferon and the metarhizium anisopliae can remarkably inhibit physiological defense of termite and improve biocontrol bacteria.
  10. 10. The use of the nucleic acid interferon of the black-chest termite according to claim 9, wherein the 3 nucleic acid interferon termites CAT dsRNA, ACSF2 dsRNA and c47753 dsRNA cooperate with beauveria bassiana or paecilomyces to control termites.

Description

Nucleic acid interferon for black chest termite and its application in preventing and controlling termite Technical Field The invention relates to the field of biotechnology, in particular to three dsRNAs (i.e. nucleic acid interferon) based on black-chest termite genes and application thereof in termite control. Background Black-chest termites (Reticulitermes chinensis) are used as civil amphibian termites widely distributed in the south of 40 ° north latitude in China, and are mainly distributed in the loyang, south yang, standing-mars, flat-top mountain, and the Zhengzhou and Xuchang kyotong regions in the southwest of Henan province. Termite nest is built in wood structure near ground or underground residual wood, has loose honeycomb structure, has activity radius up to 12m, and has feeding range over 450 square meters, and has extremely strong environment adaptability. Although the black wing termites are main hazard species in the Henan hydraulic engineering, in recent years, researches have found that the black chest termites have caused substantial hazard in hydraulic facilities such as Qin river levee, the black wing termites nest at the position of a water slope of a back water slope of a dam, the depth of the nest is generally 2-3 meters, and the soil body structure is loose by constructing crisscrossed ant channel networks, so that dangerous situations such as piping, leakage and the like can be caused in the flood season, and the structural safety of the dam is seriously threatened. In view of the challenge, the traditional chemical control method cannot meet the green control requirement of the modern hydraulic engineering because the traditional chemical control method is difficult to penetrate into the nest and can pollute the water body and destroy the ecological system. According to the requirements of the 'hydraulic engineering termite control operation guidance opinion' issued by the water conservancy department 2023, relevant departments need to strengthen dam termite research, promote technical innovation and practical application, and popularize safe and environment-friendly green control technology. Wherein, the entomopathogenic fungi (such as metarhizium anisopliae and beauveria bassiana) are not only eco-friendly and harmless to human and livestock, but also can be transmitted through contact among termites, and penetrate into the nest group to realize more thorough control effect. The insect biocontrol fungus Metarhizium (Metarhizium) is attracting attention because of its remarkable pathogenicity to various pests such as locust, grubs, etc. The biocontrol fungi have the advantages of high specificity, environmental friendliness, no residue and the like, are not easy to induce the drug resistance of pests, and have unique value in the field of pest biocontrol. However, the use of metarhizium anisopliae in the field for termite control is not ideal. Termites are typical social insects, have a multi-level defense mechanism when dealing with pathogen infection, can prevent hyphae from propagating and diffusing in blood cavities through individual physiological immunity, for example, excite an innate immune system including cellular immunity and humoral immunity through IMD, toll or JAK/STAT and other approaches, finally kill pathogen by means of immune cells, antimicrobial peptides and the like, and maintain redox balance by regulating and controlling expression of detoxification genes and antioxidant genes. In addition, termites also have a powerful group behavioral immunity, effectively removing some pathogenic substances including fungal pathogens by cleaning the surfaces of the infected peers from each other. Therefore, how to weaken the immunity of termites becomes a problem to be solved for improving the biological control effect of pathogenic bacteria. Disclosure of Invention In order to solve the defect of the prior art that the beauveria bassiana biological agent is simply used for preventing the oviductus Ranae effect, the invention provides target genes CAT, ACSF2, c47753, CAT dsRNA thereof, ACSF2 dsRNA, c47753 dsRNA thereof and application thereof in biological control of termites. Can weaken the physiological defensive ability of termites and remarkably improve the control effect on termites. A nucleic acid interferon for the black-chest termite contains three genes, namely the key gene from the antioxidizing function of black-chest termite, the key enzyme gene from the synthesis of acyl-coa in black-chest termite, and the key gene from the transcriptome database of black-chest termite. The key gene of the antioxidant function in the black-chest termite body, namely the Catalase (CAT) gene, the DNA sequence of which is derived from a black-chest termite transcriptome database and the DNA sequence of which is SEQ 1A; the key enzyme gene for catalyzing the synthesis of acyl-CoA in the body of the termite body, namely a Medium CHAIN ACYL CoA ligase (ACSF 2) gene, the DNA se