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CN-121991960-A - Common thrips death related gene MuRop and application thereof

CN121991960ACN 121991960 ACN121991960 ACN 121991960ACN-121991960-A

Abstract

The invention discloses a death-related gene MuRop of common thrips and application thereof, wherein the nucleotide sequence of the death-related gene MuRop is shown as SEQ ID NO. 1. The invention discovers that the dsRNA fed with MuRop genes of common thrips can cause death of common thrips, has good lethal effect on common thrips in different development stages, and is obviously superior to the dsRNA of other potential insect lethal related genes. And further establishes a method for preventing and treating common thrips, which can effectively prevent and treat common thrips, and provides a new thought and a new way for effectively and environmentally-friendly preventing and treating common thrips.

Inventors

  • PAN HUIPENG
  • YU YUANBIN
  • YANG CHUNXIAO
  • ZHANG CHAOQUN
  • TANG RIYUAN
  • ZHONG ZEXIN
  • LI JIANXING
  • CHEN JIAN

Assignees

  • 华南农业大学

Dates

Publication Date
20260508
Application Date
20241108

Claims (10)

  1. 1. A gene MuRop related to death of common thrips is characterized in that the nucleotide sequence of the gene is shown as SEQ ID NO. 1.
  2. 2. Use of dsRNA directed against the lethality-related gene MuRop of claim 1 for controlling common thrips.
  3. 3. Use of dsRNA directed against the death-related gene MuRop according to claim 1 for the preparation of a product for controlling common thrips.
  4. 4. The use according to claim 2 or 3, wherein the nucleotide sequence of the dsRNA is shown in SEQ ID NO. 2.
  5. 5. The dsRNA for preventing and treating common thrips is characterized in that the nucleotide sequence is shown as SEQ ID NO. 2.
  6. 6. A method of controlling common thrips comprising feeding common thrips with dsRNA directed against the death-related gene MuRop according to claim 1.
  7. 7. The method of claim 6, wherein the dsRNA has a nucleotide sequence as set forth in SEQ ID No. 2.
  8. 8. A kit for controlling common thrips comprising dsRNA directed against the death-related gene MuRop according to claim 1.
  9. 9. The kit of claim 8, wherein the dsRNA has a nucleotide sequence as set forth in SEQ ID No. 2.
  10. 10. The kit of claim 9, further comprising an in vitro transcription reagent.

Description

Common thrips death related gene MuRop and application thereof Technical Field The invention relates to the technical field of pest control, in particular to a common thrips death related gene MuRop and application thereof. Background Common thrips Megalurothrips usitatus (Bagnall) belongs to the genus Thysanoptera, thrips and thrips. The insects cause serious harm to various crops, mainly including leguminous crops such as cowpea, kidney bean and hyacinth bean, and other crops such as tomatoes, loofah, corn and the like, and particularly the occurrence and the harm in areas such as Hainan, guangdong and the like are serious. Currently, field control of common thrips mainly depends on chemical control. However, common thrips have strong concealment, so that the dosage of the common thrips is increased and the common thrips are frequently applied in the prevention and treatment process, and the common thrips have different degrees of drug resistance to common drugs. In addition, the chemical control not only kills natural enemies, pollinating insects and other beneficial organisms, but also is difficult to degrade partial chemical synthetic insecticide, thereby causing serious threat to the ecological environment. Therefore, research on an environment-friendly control method is needed to control the damage of common thrips. RNA interference (RNA INTERFERENCE, abbreviated RNAi) is a gene silencing technique triggered by endogenous or exogenous double-stranded RNA (dsRNA) that inhibits target gene expression by specifically degrading intracellular mRNA. The RNAi interference technology can control key genes of pest development or important behaviors, can indirectly block normal growth and reproduction of pests and even directly lead to death of the pests, thereby realizing the aim of pest control, being a novel green environment-friendly control method and having wide application prospect. Obtaining efficient and safe lethal target genes is a key to pest control by using RNAi technology. Rop (Ras opposite) genes play an important role in membrane fusion process of intracellular vesicle transport. The Sec1/Munc18 family proteins encoded by this gene mediate vesicle fusion by regulating the assembly of SNARE complexes. When the expression of the Rop gene is disturbed, various cell functions may be restricted, possibly resulting in blocked development or death of the insect. For example, the Rop gene deletion mutant embryos of drosophila melanogaster (Drosophila melanogaster) showed accumulation of extracellular secretion products, blocked cell proliferation, abnormal cell shape and incomplete digestion of intestinal yolk, which eventually resulted in embryonic death, failure to hatch into one-instar larvae, showing the importance of the Rop gene for drosophila embryo development. In red spiders (Tetranychus urticae), the Rop gene is critical to maintaining normal fertility, but has no significant effect on survival, and interference with the Rop gene by RNAi can cause darkening of red spiders body color and affect their excretion function. In addition, in red-anthropomorphic robusta (Tribolium castaneum), mortality rates of 11 days after dsRop injection into pupae and larvae reached 100%. RNAi interference efficiency is greatly different among different insect groups, RNAi interference efficiency of dsRNA on coleoptera and orthoptera insects is highest, RNAi is difficult to achieve by lepidoptera insects, and more researches show that RNAi efficiency of thysanoptera insects is lower. In the thrips palmi (THRIPS PALMI), the mortality rate of dsTpAPN and dsTpAPN to the thrips palmi is lower than 15%, and the mortality rate of dsTpAPN and dsTpAPN which are loaded with star-shaped cationic polymer (Star polycation, SPc) materials is only 23.3% and 30.0% compared with the control group dsGFP, so that the RNAi efficiency of thrips pests is low. At present, a high-efficiency thrips RNAi method is not researched and explored. Disclosure of Invention The invention aims to overcome the defects in the prior art and provides a common thrips death related gene MuRop and application thereof. The first object of the invention is to provide a gene MuRop related to death of common thrips. The second object of the invention is to provide the application of dsRNA aiming at the lethal related gene MuRop in preventing and controlling common thrips. The third object of the invention is to provide the application of dsRNA aiming at the lethal related gene MuRop in preparing products for preventing and controlling common thrips. The fourth object of the invention is to provide dsRNA for preventing and treating common thrips. A fifth object of the invention is to provide a method for controlling common thrips. The sixth object of the invention is to provide a kit for preventing and treating common thrips. In order to achieve the above object, the present invention is realized by the following means: the invention claims a common thrips