CN-121991961-A - Poplar TCTP gene and application thereof in regulation and control of plant insect resistance

Abstract

The invention discloses a poplar TCTP gene and application thereof in regulating and controlling plant insect resistance. The invention separates and obtains the TCTP gene capable of regulating and controlling plant insect resistance from poplar, and over-expresses or silences the TCTP gene in poplar to obtain transgenic poplar plant, which is proved by experiments to show that the transgenic poplar plant over-expressing the TCTP gene shows the property of improving insect resistance, and the transgenic poplar plant silenced with the TCTP gene shows the property of reducing insect resistance, which indicates that the TCTP gene has the property of positively regulating and controlling plant insect resistance. The invention has application prospect in improving the insect resistance of plants.

Inventors

• WANG YANWEI
• XIAO DANDAN
• Rong Kaijing
• FU TIANTIAN
• WANG ZEQI

Assignees

• 北京林业大学

Dates

Publication Date

20260508

Application Date

20241101

Claims (9)

1. 1. A TCTP gene isolated from poplar, wherein the polynucleotide sequence is set forth in (a), (b), (c) or (d): (a) A polynucleotide sequence shown in SEQ ID No. 1; (b) A polynucleotide sequence which can hybridize with the complementary sequence of the polynucleotide sequence of SEQ ID NO.1 under stringent hybridization conditions, wherein the protein encoded by the polynucleotide sequence still has the function of regulating plant insect resistance; (c) A polynucleotide sequence having at least 90% or more homology to the polynucleotide sequence shown in SEQ ID No. 1; (d) A polynucleotide variant in which one or more bases are substituted, deleted or inserted based on the polynucleotide sequence shown in SEQ ID NO.1, and the protein encoded by the polynucleotide variant still has the function of regulating plant insect resistance.
2. 2. An expression cassette or recombinant plant expression vector comprising the TCTP gene of claim 1.
3. 3. Use of the TCTP gene of claim 1, the expression cassette of claim 2 or recombinant plant expression for regulating plant insect resistance.
4. 4. The method of claim 3, wherein the controlling plant insect resistance is to increase plant insect resistance, preferably comprising overexpressing the TCTP gene in plant cells or tissues, more preferably comprising constructing a plant expression vector for the TCTP gene, and transforming plant cells or plant tissues with the plant expression vector to overexpress the TCTP gene, wherein the plant is a poplar, more preferably the poplar is the silver gland Yang Moxing line 84K.
5. 5. A method for improving the insect-resistant performance of plant and cultivating insect-resistant plant variety is characterized by comprising the steps of constructing a recombinant plant expression vector containing the TCTP gene as claimed in claim 1, transforming plant cells or plant tissues with the recombinant plant expression vector, and enabling the TCTP gene to be over-expressed in the plant cells or tissues.
6. 6. The RNAi gene of claim 1, wherein the RNAi gene has a nucleotide sequence set forth in SEQ ID No. 2.
7. 7. An interfering expression vector comprising the RNAi gene of claim 6.
8. 8. Use of the RNAi gene of claim 6, or the interfering expression vector of claim 7, for modulating insect resistance in a plant.
9. 9. The use according to claim 8, wherein the control of plant pest resistance is a decrease in plant pest resistance, wherein the plant is a poplar, preferably the poplar is the silver gland Yang Moxing line 84K.

Description

Poplar TCTP gene and application thereof in regulation and control of plant insect resistance Technical Field The invention relates to a TCTP gene and application thereof, in particular to a TCTP gene separated from poplar and application thereof in regulating and controlling plant insect resistance, belonging to the field of TCTP genes and application thereof. Background Poplar is one of the most widely distributed and adaptable forest tree species in the world. Poplar is widely used as a model tree species for genetic improvement of trees due to its fast growth rate, high yield and high regeneration efficiency. It plays an important role in the economic and global ecosystems, but poplar is often affected by various biotic stresses, especially insect pests, during growth due to large-area single forestation. Fall webworm is a quarantine pest which seriously damages trees and is widely distributed in European and Asian areas. The feeding of fall webworm can cause the potential weakness of poplar, the stress resistance is weakened, the yield and income are reduced, even the absolute yield is realized, the fall webworm is called as 'smokeless fire', and great harm is generated to the growth and survival of forest trees. At present, the genetic engineering of the resistance of the fall webworm is mostly introduced with exogenous insect-resistant genes, such as Bt genes, and identification and functional research on the endogenous insect-resistant genes of poplar are not seen. The translational control tumor proteins (TCTP, also known as p21, p23, translationally controlled tumor protein) are proteins commonly expressed and distributed in eukaryotes and play a vital role in plant growth and stress response. At present, the research on the resistance of TCTP gene regulation forest tree to fall webworm is not reported. In view of the importance of the regulatory function of the TCTP gene and the wide regulatory range, if the TCTP gene is separated from poplar by using a genetic engineering technology and an RNAi sequence vector for constructing an over-expression vector or silencing the TCTP gene is transferred into the poplar, the method is helpful for breeding research of poplar pest resistance, helps to maintain ecological safety and plays an important role in promoting the stable development of the poplar industry. Disclosure of Invention It is an object of the present invention to provide TCTP genes isolated from poplar; It is a second object of the present invention to provide RNAi genes that silence or inhibit the expression of the TCTP gene; It is a third object of the present invention to provide an expression cassette or recombinant expression vector containing a TCTP gene or an RNAi gene silencing expression of the TCTP gene; The fourth object of the present invention is to apply TCTP gene, RNAi gene for silencing expression of TCTP gene, expression cassette or expression vector containing RNAi gene for silencing expression of TCTP gene to regulate plant insect resistance. In order to achieve the above purpose, the main technical scheme adopted by the invention comprises: in one aspect of the present invention, there is provided a TCTP gene isolated from poplar, having a polynucleotide sequence as set forth in (a), (b), (c) or (d): (a) A polynucleotide sequence shown in SEQ ID No. 1; (b) A polynucleotide sequence which can hybridize with the complementary sequence of the polynucleotide sequence of SEQ ID NO.1 under stringent hybridization conditions, wherein the protein encoded by the polynucleotide sequence still has the function of regulating plant insect resistance; (c) A polynucleotide sequence having at least 90% or more homology to the polynucleotide sequence shown in SEQ ID No. 1; (d) A polynucleotide variant in which one or more bases are substituted, deleted or inserted based on the polynucleotide sequence shown in SEQ ID NO.1, and the protein encoded by the polynucleotide variant still has the function of regulating plant insect resistance. In another aspect of the present invention, there is provided an RNAi gene for silencing expression of TCTP gene, wherein the nucleotide sequence of the RNAi gene is shown as SEQ ID No. 2. By "variant" is meant a substantially similar sequence, and for a polynucleotide, the variant comprises a deletion, insertion, or/and substitution of one or more nucleotides at one or more positions in the native polynucleotide. For polynucleotides, conservative variants include those that do not alter the encoded amino acid sequence due to the degeneracy of the genetic code. Such naturally occurring variants can be identified by existing molecular biology techniques. Variant polynucleotides also include polynucleotides of synthetic origin, such as variants of polynucleotides which still encode TCTP proteins obtained using site-directed mutagenesis or by recombinant means (e.g., DNA shuffling). The function or activity of the protein encoded by the variant polynucleotide