Search

CN-121991962-A - Cold-resistant gene CTB3 of rice and application thereof in screening and creating cold-resistant rice

CN121991962ACN 121991962 ACN121991962 ACN 121991962ACN-121991962-A

Abstract

Provides a rice cold-resistant gene CTB3 and application of a promoter thereof in rice cold-resistant characters. The application also provides application of the rice cold-resistant gene CTB3 and a promoter thereof in screening and creating cold-resistant rice.

Inventors

  • LI JINJIE
  • LI JIN
  • GUO HAIFENG
  • CHEN CHAO
  • SUN XINGMING
  • ZHANG ZHANYING
  • ZHANG HONGLIANG
  • LI ZICHAO

Assignees

  • 中国农业大学
  • 中国种子集团有限公司

Dates

Publication Date
20260508
Application Date
20241101

Claims (10)

  1. 1. A rice cold tolerance gene CTB3 comprising the nucleotide sequence shown in SEQ ID No.1 or comprising a nucleotide sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more than 99% identity to SEQ ID No. 1.
  2. 2. The rice cold tolerance gene CTB3 according to claim 1, which consists of the nucleotide sequence shown in SEQ ID NO. 1.
  3. 3. A protein encoded by rice cold tolerance gene CTB3, said protein comprising the amino acid sequence shown in SEQ ID No. 2 or comprising an amino acid sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more than 99% identity to SEQ ID No. 2.
  4. 4. A nucleic acid encoding the protein of claim 3.
  5. 5. An expression vector comprising the rice cold tolerance gene CTB3 of claim 1 or 2 or the nucleic acid of claim 4.
  6. 6. A transgenic method comprising transforming a cell of rice with the rice cold tolerance gene CTB3 of claim 1 or 2, the nucleic acid of claim 4 or the expression vector of claim 5, and selecting a rice cell having the rice cold tolerance gene CTB3, the nucleic acid or the expression vector incorporated in the genome.
  7. 7. The transgenic method of claim 6, wherein the rice is selected from the group consisting of rice, upland rice, indica, japonica, early, late, sticky, glutinous, she Youmao, and She Momao.
  8. 8. An Indel molecular marker for screening cold-tolerant rice germplasm, selected from the group consisting of a first Indel molecular marker and a second Indel molecular marker, wherein the first Indel molecular marker is obtained by PCR amplification from a pair of primers shown in SEQ ID nos. 5 and 6, and wherein the second Indel molecular marker is obtained by PCR amplification from a pair of primers shown in SEQ ID nos. 7 and 8.
  9. 9. The Indel molecular marker of claim 8, wherein the rice is indicated to be cold tolerant when the PCR product is about 272bp and/or about 735 bp.
  10. 10. The Indel molecular marker of claim 8 or 9, wherein the rice is selected from the group consisting of rice, upland rice, indica rice, japonica rice, early rice, late rice, sticky rice, glutinous rice, rice She Youmao and rice She Momao.

Description

Cold-resistant gene CTB3 of rice and application thereof in screening and creating cold-resistant rice Technical Field The application relates to the technical field of rice molecular genetic breeding, in particular to a rice CTB3 gene and application thereof in cold-resistant rice screening and creation. Background Rice is a major food crop originating in tropical regions and is very sensitive to low temperatures. The planting area of the warm-zone japonica rice is wider, and the warm-zone japonica rice can be planted in cold areas with high altitude and high latitude, compared with the warm-zone japonica rice, the indica rice and the tropical japonica rice are suitable for being planted in areas with more sufficient illumination and temperature. In the background of more frequent global extreme weather events, low temperature stress seriously threatens the safety of rice production. It is estimated that the yield of chinese rice will be lost 300-500 ten thousand tons due to the low temperature in autumn. The yield of chinese rice was reduced by about 3.7% by extreme coldness between 1999 and 2012. The cold tolerance of booting stage directly determines the yield of rice under cold stress, so that the cold tolerance of the rice is very urgent to excavate and utilize the cold tolerance gene of booting stage to improve. The low-temperature cold injury of rice mainly refers to the phenomenon that the rice is affected by low temperature below the lowest critical temperature of continuous or short-term low temperature, so that the growth of the rice is delayed, and nutrition or reproductive organs are destroyed when serious, so that the rice cannot normally develop to reduce the yield. The rice is easy to suffer from low-temperature cold injury in the whole growth period. According to the period of low temperature, the cold injury in the vegetative growth period (germination period and seedling period) and the cold injury in the reproductive growth period (booting period and flowering period) can be divided. The booting stage is a key stage of meiosis and microsporogenesis of pollen mother cells of rice, and the rice is most sensitive to low temperature in the stage. Cold injury during booting stage affects the differentiation and development of microspores, and causes pollen abortion and reduced fruiting rate, and directly affects yield. The cold-resistant locus or gene of the booting stage of the rice is excavated and cloned, so that molecular breeding is developed, and the loss of the yield of the rice caused by cold damage of the booting stage is reduced. At present, only five booting stage cold-resistant genes are identified by natural populations. CTB1 is the first cloned booting stage cold tolerance gene, and positively regulates booting stage cold tolerance, but the mechanism of action is still unknown. CTB4a and AtpB interact to increase the setting rate of rice in high altitude areas by increasing energy supply, CTB2 maintains cell membrane permeability by increasing the levels of sterols and acylsterols, and damage to pollen grains and pollen walls is reduced, CTB2 and CTB4a together promote rice planting in high altitude areas such as Yunnan plateau areas. The OsLEA9 can negatively regulate and control the cold resistance of the booting stage, and can improve the cold resistance of the booting stage through gene editing. qCTB7 is used for positively regulating and controlling the cold resistance of booting stage, and qCTB7 dominant haplotype is more beneficial to rice breeding in high latitude areas. Cold tolerance of rice in booting stage is a complex quantitative character, and the genes are still insufficient for molecular breeding of cold tolerance of rice. Therefore, the identification of the new booting stage cold-resistant gene has very important theoretical and practical significance for cultivating new varieties of cold-resistant rice. Disclosure of Invention The inventor of the application discovers a new cold-tolerant gene CTB3 for controlling cold tolerance of rice in booting stage for the first time and promoter function variation and related applications thereof. The gene can positively regulate and control the cold tolerance of rice in booting stage, and the promoter has 2 Indel variations among indica rice and japonica rice subgroups to regulate the expression and regulate the cold tolerance. The cold tolerance of rice varieties in booting stage can be improved or screened by using the gene and 2 molecular markers on the promoter, so that the planting area of rice is enlarged, and the stable yield of late season rice in China is ensured. In particular, the application provides the following technical scheme. In a first aspect, the present application provides a rice cold tolerance gene CTB3 comprising the nucleotide sequence shown in SEQ ID No. 1 or comprising a nucleotide sequence having at least 80%, 85%, 90%, 95%, 96%, 97%, 98%, 99% or more than 99% identity to SEQ ID No. 1. In a