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CN-121991968-A - Rice heat shock transcription factor OsHSFC a and application of coding gene thereof in rice disease-resistant breeding

CN121991968ACN 121991968 ACN121991968 ACN 121991968ACN-121991968-A

Abstract

The invention discloses a rice heat shock transcription factor OsHSFC a and a coding protein and application thereof, wherein the gene OsHSFC a provided by the invention is a DNA molecule shown in 1) SEQ ID NO.1, 2) SEQ ID NO.2, 3) a DNA molecule which hybridizes with a DNA sequence limited by 1) or 2) under strict conditions and codes the protein, and 4) a DNA molecule which has more than 90% homology with a DNA sequence limited by 1) or 2) or 3) and codes a plant heat shock transcription factor. The invention also provides a protein encoded by the gene, and the protein affects disease resistance of plants. The coding gene of the protein is knocked out in plants with normal disease resistance, so that transgenic plants with enhanced disease resistance can be cultivated. The protein and the coding gene thereof can be applied to plant genetic improvement.

Inventors

  • WAN JIANMIN
  • ZHOU LEI
  • JIANG LING
  • LI KUNHAO
  • WANG CHENGYU
  • SHI XIAOCHUAN
  • FENG HEYAN
  • HE JUN
  • LIU SHIJIA
  • YU TING

Assignees

  • 南京农业大学

Dates

Publication Date
20260508
Application Date
20251229

Claims (9)

  1. The OsHSFC1a gene is characterized in that OsHSFC a gene is selected from one of DNA molecules shown in the following 1), 2), 3) or 4): 1) A DNA molecule shown in SEQ ID No. 1; 2) A DNA molecule shown in SEQ ID No. 2; 3) A DNA molecule which hybridizes under stringent conditions to the DNA sequence defined in 1) or 2) and which codes for a protein according to SEQ ID NO. 3; 4) A DNA molecule which has more than 90% homology with the DNA sequence defined in 1) or 2) or 3) and codes for a protein related to a heat shock transcription factor.
  2. 2. A protein encoded by the OsHSFC a gene of claim 1.
  3. 3. The protein of claim 2, selected from any one of the following (a) or (b): (a) A protein consisting of the amino acid sequence shown in SEQ ID NO. 3; (b) And (3) the protein which is derived from the SEQ ID NO.3, is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence of the SEQ ID NO.3 and is related to disease resistance.
  4. 4. The knock-out vector of OsHSFC a gene according to claim 1.
  5. 5. The knockout vector of claim 4, wherein the knockout vector is a CRISPR-Cas9 vector, and the target sequence of the CRISPR-Cas9 vector is a DNA molecule represented by SEQ ID No.1 or SEQ ID No. 2.
  6. 6. A recombinant expression vector, cassette or recombinant bacterium comprising the OsHSFC a gene of claim 1.
  7. 7. Use of the OsHSFC a gene of claim 1, or the protein of claim 2 or 3, or the knockout vector of claim 4 or 5, or the recombinant expression vector, expression cassette or recombinant bacterium of claim 6 in breeding disease-resistant rice.
  8. 8. The use according to claim 7, wherein the disease resistance is against rice blast and bacterial leaf blight.
  9. 9. The use according to claim 7, wherein the knock-out vector of claim 4 or 5 is capable of increasing disease resistance in rice by knocking out OsHSFC a gene in rice, or reducing the expression level of the protein of claim 2 or 3 in rice, or transferring the vector into rice.

Description

Rice heat shock transcription factor OsHSFC a and application of coding gene thereof in rice disease-resistant breeding Technical Field The invention belongs to the field of genetic engineering, and particularly relates to a rice disease-resistant gene OsHSFC a, and a coding protein and application thereof. Background Rice (Oryza satival.) is a grain in more than 50% of the world population, and is also an important grain crop in China, and the rice production is very important in national economy. Over-reliance on chemical pesticides not only increases cost and environmental pressure, but also faces the challenge of disease variation leading to failure of resistance. Therefore, creating a new variety with broad spectrum and lasting disease resistance is a necessary way to fundamentally realize green sustainable rice production. Cultivating disease-resistant rice varieties is important to guaranteeing national grain safety. Heat shock transcription factors are a group of special heat shock proteins which are rapidly synthesized by scientists in the last 60-70 th century to protect cells from damage when the organisms are found to be subjected to high temperatures and other adverse conditions. Whereas studies confirm that there is a conserved DNA sequence upstream of the gene for heat shock proteins, called the heat shock element. Whereas, at the end of the 80 s to the beginning of the 90 s of the last century, researchers first isolated and identified proteins that bind HSE, i.e., heat shock transcription factors, from yeast and drosophila. Subsequently, the first HSF gene (from yeast) was cloned in 1990. In plants, however, plant HSF families are abnormally large, much more than animals (only 3 in humans) as revealed by whole genome sequencing. Up to 25 OsHsf members in rice. This suggests that plant HSF may be highly functionally differentiated and that the tissue expression patterns of the different OsHsf members are different and respond differently to various stresses such as high temperature, drought, high salt, low temperature and pathogenic bacteria infection, indicating the broad scope of their function. Plant HSFs are classified into three major classes A, B, C, based on their structural features (e.g., oligomerization and transcription activation domains), of which class a and class B are the heart of the study. The OsHsf family of rice also follows this classification. However, there is still little research on HSF class C. Therefore, a system is needed to analyze novel disease-resistant mechanisms of crops, the key problem of scarcity of disease-resistant genetic resources is overcome, molecular modules and breeding ways which can be used for disease resistance improvement are explored, and novel germplasm with continuous disease resistance is created. Disclosure of Invention In order to solve the technical problems in the prior art, the invention provides a rice heat shock transcription factor OsHSFC a and application of a coding gene thereof in rice disease-resistant breeding. The technical scheme of the invention is as follows: it is a first object of the present invention to provide OsHSFC a gene, said OsHSFC a gene being selected from one of the DNA molecules shown in 1), 2), 3) or 4) below: 1) A DNA molecule shown in SEQ ID No. 1; 2) A DNA molecule shown in SEQ ID No. 2; 3) A DNA molecule which hybridizes under stringent conditions to the DNA sequence defined in 1) or 2) and which codes for a protein according to SEQ ID NO. 3; 4) A DNA molecule which has more than 90% homology with the DNA sequence defined in 1) or 2) or 3) and codes for a protein related to a heat shock transcription factor. A second object of the present invention is to provide a protein encoded by the OsHSFC a gene described above. Further, selected from any one of the following (a) or (b): (a) A protein consisting of the amino acid sequence shown in SEQ ID NO. 3; (b) And (3) the protein which is derived from the SEQ ID NO.3, is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence of the SEQ ID NO.3 and is related to disease resistance. A third object of the present invention is to provide a knock-out vector of the OsHSFC a gene described above. Further, the knockout vector is a CRISPR-Cas9 vector, and the target sequence of the CRISPR-Cas9 vector is a DNA molecule shown as SEQ ID NO.1 or SEQ ID NO. 2. The fifth object of the present invention is to provide the use of OsHSFC a gene, protein, or knock-out vector as described above in breeding disease-resistant rice. Further, the disease resistance is rice blast resistance and bacterial leaf blight resistance. Furthermore, the rice disease resistance can be mentioned by knocking out OsHSFC a gene in rice, or reducing the expression level of the protein in rice, or transferring the knock-out vector into rice. Advantageous effects The invention discovers that the related protein of the heat shock transcription fa