CN-121991971-A - Plant selenium accumulation and salt stress tolerance regulation gene GmLsi L and application thereof

CN121991971ACN 121991971 ACN121991971 ACN 121991971ACN-121991971-A

Abstract

The invention discloses a plant selenium accumulation and salt stress tolerance regulation gene GmLsi L and application thereof, belonging to the field of genetic engineering. The nucleotide sequence of the gene is shown as SEQ ID NO. 5, and the amino acid sequence is shown as SEQ ID NO. 6. Is primarily localized to the endoplasmic reticulum. Experiments prove that the over-expression of GmLsi L genes can remarkably improve the selenium accumulation capacity of arabidopsis, the total selenium content is remarkably increased compared with that of a wild type after 40 mu M Na 2 SeO 3 treatment, and meanwhile, the tolerance of plants to salt stress, selenium stress and compound stress is enhanced, and the fresh weight and chlorophyll content of an over-expression strain are better than those of the wild type. The gene can be applied to genetic improvement of crops, can improve the nutritional value and stress resistance of agricultural products, and provides a new path for optimizing crop varieties such as soybeans and utilizing saline-alkali soil.

Inventors

LI LI
WANG YE
ZHANG NA
XIONG YUZHOU
ZHANG XIAOYUN
ZHANG BAOCHEN

Assignees

武汉轻工大学

Dates

Publication Date: 20260508
Application Date: 20260309

Claims (9)

1. A plant selenium accumulation and salt stress tolerance regulating gene GmLsi L is characterized in that the nucleotide sequence of the gene GmLsi L is shown as SEQ ID NO. 5 or a nucleotide sequence which has more than 90% homology with the sequence shown as SEQ ID NO. 5 and codes the same functional protein.
2. A protein encoded by the gene GmLsi L as claimed in claim 1, wherein the amino acid sequence of the protein is shown in SEQ ID NO. 6.
3. A recombinant expression vector comprising the gene GmLsi L of claim 1.
4. A host cell comprising the recombinant expression vector of claim 3, wherein the host cell is escherichia coli DH 5a, agrobacterium EHA105, or a plant cell.
5. Use of the gene GmLsi L of claim 1, or the protein of claim 2, or the expression vector of claim 3, or the host cell of claim 4, for regulating selenium accumulation and/or salt tolerance in a plant.
6. The use according to claim 5, wherein gene GmLsi L is introduced into the plant for overexpression, to increase the uptake and accumulation of selenium in the plant and/or to increase the salt tolerance of the plant.
7. The use according to any one of claims 5-6, wherein the plant comprises soybean, arabidopsis, tomato or maize.
8. A method of growing a transgenic plant with high selenium accumulation comprising using genetic engineering means to overexpress the gene of claim 1 in the plant or to increase the protein content or activity of claim 2 in the plant.
9. A method for improving salt tolerance in a plant, comprising overexpressing the gene of claim 1 in the plant or increasing the content or activity of the protein of claim 2 in the plant by genetic engineering means.

Description

Plant selenium accumulation and salt stress tolerance regulation gene GmLsi L and application thereof Technical Field The invention relates to the technical field of genetic engineering, in particular to a plant selenium accumulation and salt stress tolerance regulation gene GmLsi L and application thereof. Background Selenium (Se) is a trace element essential for human and animal health maintenance, and is also a beneficial element for plants. The enhancement of dietary selenium intake of human body through crop selenium enhancement is a hot problem studied in recent years, and can not only improve the selenium deficiency condition of human body, but also help plants to cope with various adverse environmental pressures. Therefore, the novel gene with selenium accumulation regulation and control and saline-alkali tolerance functions is excavated and applied to soybean genetic improvement, and becomes a key for breaking through the bottleneck of the prior art and realizing soybean yield improvement and quality optimization. Disclosure of Invention In view of the above, the present invention aims to provide a plant selenium accumulation and salt stress tolerance regulatory gene GmLsi L and its application. The present invention is based on transcriptome data sequenced by NGS (next-generation sequencing) and SMRT (single-molecular real-time) cloned from soybean variety "dongnong 690" into a new Lsi2-like gene (designated GmLsi L) which is located on chromosome 16 along with another 2 Lsi2 homologous genes. The analysis result of the phylogenetic tree shows that soybean GmLsi L has closer relatedness to Lsi2 protein in Arabidopsis thaliana and tomato and farthest relatedness to Lsi2 of horsetail, and neither the Arabidopsis thaliana nor the tomato has silicon transport activity, and the functions of the soybean GmLsi L are unknown at present. Analysis of cis-acting elements of a gene promoter region shows that a large number of promoter elements involved in stress response and hormone response, including response elements of ABA, biotin, jasmonic acid, ethylene, salicylic acid, gibberellin, drought stress, high-salt stress and the like exist in GmLsi L promoter region. In a first aspect of the invention, a plant selenium accumulation and salt stress tolerance regulation gene GmLsi L is provided, the nucleotide sequence of the gene GmLsi L is shown as SEQ ID NO. 5, or a nucleotide sequence which has more than 90% homology with the sequence shown as SEQ ID NO. 5 and codes the same functional protein. In a second aspect of the present invention, there is provided a protein encoded by the above gene GmLsi L, the amino acid sequence of which is shown in SEQ ID NO. 6. In a third aspect of the present invention, there is provided a recombinant expression vector comprising the gene GmLsi L described above. In a fourth aspect of the invention, a host cell is provided, comprising the recombinant expression vector described above, wherein the host cell is E.coli DH 5. Alpha., agrobacterium EHA105 or a plant cell. In a fifth aspect, the invention provides the use of said gene GmLsi L, or said protein, or said expression vector, or said host cell, for modulating selenium accumulation and/or salt tolerance in a plant. Further, gene GmLsi L is introduced into plants to be overexpressed, so that the selenium absorption and accumulation capacity of the plants is improved and/or the salt tolerance of the plants is improved. Further, the plant comprises soybean, arabidopsis, tomato or maize. In a sixth aspect of the present invention, there is provided a method for growing transgenic plants with high selenium accumulation, comprising using genetic engineering means to overexpress said genes in plants or to increase the content or activity of said proteins in plants. In a seventh aspect of the present invention, there is provided a method for improving salt tolerance of a plant, comprising using genetic engineering means to overexpress said gene in the plant or to increase the content or activity of said protein in the plant. The beneficial effects of the invention at least comprise: (1) The GmLsi L gene cloned by the invention can obviously enhance the selenium absorption and accumulation capacity of plants, the total selenium content of the arabidopsis overexpression strain is obviously improved compared with that of wild type after the arabidopsis overexpression strain is treated by 40 mu M Na 2SeO3, the key functional gene is provided for crop selenium biological enhancement, the selenium nutrition level of agricultural products can be effectively improved, and the dietary selenium intake requirement of human bodies is met. (2) The GmLsi L gene provided by the invention can simultaneously improve the tolerance of plants to salt stress and selenium stress, and under 150 mM NaCl single salt, 20 mu M Na 2SeO3 single selenium and salt+selenium compound stress, the fresh weight and chlorophyll content of the over-expression strain