CN-121991972-A - Tea tree expansin CsEXPA gene and application thereof in regulating and controlling cold resistance of tea tree

CN121991972ACN 121991972 ACN121991972 ACN 121991972ACN-121991972-A

Abstract

The invention belongs to the technical field of genetic engineering, and discloses a tea tree expansin CsEXPA gene and application thereof in regulating and controlling cold resistance of tea trees. The gene is tea EXPANSIS family gene, the nucleotide sequence is shown as SEQ ID NO.1, and the coding protein sequence is shown as SEQ ID NO. 2. The gene is in a down-regulation expression mode at a low temperature of 4 ℃, is a negative regulation factor of tea tree low-temperature response, and can aggravate tea tree leaf low-temperature damage by over-expression, and can obviously relieve tea tree young shoot low-temperature damage by inhibiting the expression by an antisense oligonucleotide technology. The invention also constructs recombinant plasmid and transgenic engineering bacteria containing the gene, firstly defines the function of the gene in low-temperature stress response of tea trees, fills up the research blank of the expansin in the field of cold-resistant regulation of tea trees, provides a new thought for cold-resistant mechanism research of tea trees, and also provides gene targets and technical support for genetic improvement of cold-resistant resistance characters of tea trees.

Inventors

TONG WEI
Yi Lianghui
Xia Enhua
LI FANGDONG
ZHU YUQING
ZHAO WENSHI
MA LINHONG

Assignees

安徽农业大学

Dates

Publication Date: 20260508
Application Date: 20260318

Claims (6)

1. The tea tree expansin CsEXPA gene is characterized in that the tea tree expansin CsEXPA gene is a tea tree EXPANSIS family gene, and the nucleotide sequence of the tea tree expansin CsEXPA gene is shown in a sequence table SEQ ID NO. 1.
2. A tea tree expansin CsEXPA gene according to claim 1 wherein the amino acid sequence of the protein encoded by the tea tree expansin CsEXPA gene is as shown in sequence table SEQ ID No. 2.
3. A recombinant plasmid containing the tea tree expansin CsEXPA gene according to claim 1, wherein said recombinant plasmid comprises the PGEX4T-1: csEXPA10 plasmid obtained by ligating CsEXPA gene to PGEX4T-1 Vector vector or CsEXPA-PCAMBIA 1305 vector obtained by ligating CsEXPA gene to PCAMBIA1305 vector.
4. A transgenic engineering bacterium containing the recombinant plasmid of claim 3, wherein the transgenic engineering bacterium is an engineering bacterium obtained by transforming the recombinant plasmid into escherichia coli DH5 alpha or an engineering bacterium obtained by transforming the recombinant plasmid into agrobacterium GV 3101.
5. Use of the tea tree expansin CsEXPA gene according to claim 1 for regulating cold tolerance of tea tree, wherein expression of CsEXPA gene in tea tree is inhibited and low temperature tolerance of tea tree is improved.
6. The application of the tea tree expansin CsEXPA gene in regulating and controlling cold resistance of tea trees according to claim 5, wherein the application is applicable to low-temperature stress scenes caused by frost and cold in the spring, the low-temperature range is-2 ℃ to 4 ℃, and the application is used for genetically improving and breeding of the cold resistance of tea trees.

Description

Tea tree expansin CsEXPA gene and application thereof in regulating and controlling cold resistance of tea tree Technical Field The invention relates to the technical field of genetic engineering, in particular to a tea tree expansin CsEXPA gene and an application thereof in regulating and controlling cold resistance of tea trees. Background Tea tree (CAMELLIA SINENSIS (L.) Kuntze) is an important cash crop which mainly picks up tender buds and likes Wen Wei cold, tea can be processed into drink, contains various beneficial components and has health care effect, and the development of tea industry has both civil and national economic values. In recent years, tea trees in winter and early spring frequently suffer from low-temperature injuries such as frost, cold in the spring and the like, after new shoots of the tea trees are damaged, the yield and the quality of the tea trees can be greatly reduced, and the tea trees can die when serious, and the low temperature becomes one of the most important and serious natural disasters in the tea production, so that the health sustainable development of the tea industry is seriously threatened. Expansins (EXPANSIS) are an important component of plant cell walls, and are widely found in various plant cell tissues. The content of the expansin is lower under the normal growth condition of the plant, and the expansin can be rapidly induced to be up-regulated by several times or even hundreds of times when the plant is in a specific development stage or is stimulated by external environmental factors. The existing research shows that the expansin is involved in a plurality of growth and development links such as plant seed germination, initiation and extension of root hairs, leaf growth and development, leaf stalk drop, pollen tube extension, fruit maturation and the like, and is also involved in a plurality of stress resistance reactions such as drought resistance, disease resistance, high temperature resistance and the like of plants. However, up to now, the role of the expansin in the tea tree response low temperature stress process is not clear, and the specific genes for the tea tree cold resistance genetic improvement are lacking, so that effective support cannot be provided for the tea tree cold resistance mechanism research from the gene level, and directional breeding of the tea tree cold resistance character is difficult to realize, which becomes a key problem for restricting the development of the tea tree cold resistance breeding technology. Disclosure of Invention The invention aims to provide a tea tree expansin CsEXPA gene and application thereof for regulating and controlling cold resistance of tea trees, overcomes the defect that the expansin has an unknown function in low-temperature stress response of the tea trees in the prior art, clearly determines the regulation and control relationship between the CsEXPA gene and the cold resistance of the tea trees, provides a new thought for research on cold resistance mechanisms of the tea trees, provides specific gene targets and practical application schemes for breeding of cold resistance characters of the tea trees, and solves the technical problems of yield and quality reduction of the tea trees due to low-temperature stress. In order to achieve the above object, the present invention provides the following technical solutions: the invention provides a tea tree expansin CsEXPA gene, wherein the tea tree expansin CsEXPA gene is a tea tree EXPANSIS family gene, and the nucleotide sequence of the tea tree expansin CsEXPA gene is shown in a sequence table SEQ ID NO. 1. Preferably, the amino acid sequence of the protein coded by the tea tree expansin CsEXPA gene is shown in a sequence table SEQ ID NO. 2. A recombinant plasmid containing tea tree expansin CsEXPA gene, which comprises PGEX4T-1 obtained by connecting CsEXPA gene to PGEX4T-1 Vector vector CsEXPA plasmid or CsEXPA-PCAMBIA vector obtained by connecting CsEXPA gene to PCAMBIA1305 vector. A transgenic engineering bacterium containing a recombinant plasmid is an engineering bacterium obtained by transforming the recombinant plasmid into escherichia coli DH5 alpha or an engineering bacterium obtained by transforming the recombinant plasmid into agrobacterium GV 3101. Application of tea tree expansin CsEXPA gene in regulating cold resistance of tea tree, inhibiting expression of CsEXPA gene in tea tree body, and improving low temperature tolerance of tea tree. Preferably, the application is suitable for low-temperature stress scenes caused by frost and cold in the spring, the temperature range of the low temperature is-2 ℃ to 4 ℃, and the application is used for genetic improvement breeding of the cold resistance character of the tea tree. The invention has the beneficial effects that: (1) The invention clones and verifies the gene CsEXPA of the expansin for regulating the tea tree to respond to the low-temperature stress for the first time, and definitely