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CN-121991986-A - Rice disease spot mutant lrs3 and application thereof

CN121991986ACN 121991986 ACN121991986 ACN 121991986ACN-121991986-A

Abstract

The invention belongs to agricultural biotechnology engineering, and particularly relates to a rice disease spot gene LRS3 and application thereof in rice breeding. The invention discloses a rice lesion gene LRS3, the DNA sequence is shown in SEQ ID NO. 1, and the cDNA sequence is shown in SEQ ID NO. 2. The invention also provides a rice disease spot LRS3 mutant which is a mutant of LOC_Os03g06410 gene, wherein the mutation is a change of protein structure translated by LRS3 gene caused by single base substitution, so that the function of the protein is changed. The rice disease spot mutant influences the growth and development of rice, changes the agronomic characters of the rice, and can be applied to rice breeding.

Inventors

  • Deng Jiarui
  • QU JIALE
  • LIN CHENGDE
  • YE SHUANG
  • XIONG WENHUI
  • LIAN JINJIN
  • Mu Siyi
  • XU JIANGMIN
  • LUO YILIN
  • LIU FUYUAN
  • GE YIJUN
  • Zhu Engao
  • RAO YUCHUN

Assignees

  • 浙江师范大学

Dates

Publication Date
20260508
Application Date
20260205

Claims (9)

  1. 1. The rice disease spot gene LRS3 is characterized in that the DNA sequence of the rice disease spot gene LRS3 is shown in SEQ ID NO. 1.
  2. 2. The rice lesion gene LRS3 according to claim 1, wherein the cDNA sequence of the rice lesion gene LRS3 is shown in SEQ ID NO. 2.
  3. 3. The protein encoded by the rice lesion gene LRS3 according to claim 1 or 2, wherein the amino acid sequence is shown in SEQ ID NO. 3.
  4. 4. The gene of the mutant material lrs3 is characterized in that the mutant DNA sequence is shown as SEQ ID NO. 4, and the cDNA sequence is shown as SEQ ID NO. 5.
  5. 5. The application of genes of the rice lesion gene LRS3 and the mutant material LRS3 is characterized by affecting the growth and development of rice.
  6. 6. The application of genes of the rice lesion gene LRS3 and the mutant material LRS3 is characterized in that the application of the genes is characterized in that the agronomic characters are changed and the genes are applied to rice breeding.
  7. 7. The method of using the genes of LRS3 and lrrs 3 as defined in claim 5 or 6, wherein the plant height, tillering number, ear length, seed setting rate and grain size of rice are affected.
  8. 8. The method of using the gene LRS3 or LRS3 as defined in claim 5 or 6, wherein the agronomic trait comprises red brown rust.
  9. 9. A method for regulating growth and development of rice and changing agronomic traits, characterized in that the rice lesion gene LRS3 as claimed in claim 1 or 2 and the gene of the mutant material LRS3 as claimed in claim 4 are utilized.

Description

Rice disease spot mutant lrs3 and application thereof Technical Field The invention belongs to agricultural biotechnology engineering, and particularly relates to a rice disease spot gene LRS3 and application thereof in rice breeding. Background The rice disease spots (Rice lesion mimic) refer to necrotic or chlorosis spots similar to diseases on organs such as leaves when rice is not infected by obvious pathogenic bacteria, and the phenotype of the rice disease spots is diversified, wherein the rice disease spots can be classified into brown, white, red and the like according to colors, can be in dot-like, strip-like and irregular shapes according to shapes, have obvious size differences, and part of disease spots can be gradually enlarged or connected with [1] along with plant growth. The disease-like spots are generated from the tillering stage to the grouting stage of the rice, and the disease-like spots appear in the lower functional leaves in the initial stage and expand to the upper leaves along with the growth process. Its occurrence has space-time specificity, part of mutant type lesions are only expressed under specific temperature (25-30 ℃) or illumination condition (12-14 h illumination), and the lesion formation process is accompanied with typical characteristics of Programmed Cell Death (PCD), such as nucleus shrinkage, cytoplasmic degradation, etc. [2]. In contrast, the disease spots of the rice can be accurately prevented and controlled, the photosynthetic efficiency and the yield stability of the rice can be effectively ensured, and the economic and ecological benefits of grain production are further maintained. The leaf is used as the core organ for photosynthesis and accumulation of photosynthesis products of rice, and is also the key part for sensing external stress and starting defense response of the plant, and the integrity and physiological functions of the leaf directly determine the mass production capacity [3] of the rice. Along with the appearance and expansion of the disease spots on the leaves, chloroplast structures in the leaves are damaged firstly, thylakoid membranes are disintegrated, basal grains are arranged in disorder, and simultaneously, the degradation of photosynthetic pigments and the abnormal decomposition of macromolecular substances such as proteins, nucleic acids and the like are accompanied, in addition, excessive accumulation of Reactive Oxygen Species (ROS) is caused in the disease spot area, the oxidation-reduction balance in cells is broken, and the cell damage [4] is further aggravated. The leaves bear the core functions of providing carbohydrates and nutrient substances for root growth, spike development and grain grouting in key growth stages of rice tillering, jointing, grouting and the like, and the substances are transported to storehouse organs such as young spikes, grains and the like through phloem, so that the forming [5] of the fruiting rate and thousand grain weight is directly influenced. Therefore, the occurrence degree and distribution characteristics of leaf lesions can be generally used as important indexes for assessing the health condition of rice and predicting the yield potential. The formation of the rice lesion is not a random process, but is regulated and controlled by various intrinsic factors, such as the signal balance of the expression regulation hormone of the lesion-related genes (OsLSD 1, osPAL and the like), and is also obviously influenced by external environmental factors, such as pathogenic bacteria infection, extreme temperature and light conditions, soil nutrient imbalance, heavy metal stress and the like [6][7]. Notably, some rice leaf spot-like mutants or leaf spot-like-induced plants may exhibit an enhanced resistance effect against bacterial leaf spot banded sclerotial disease (Xanthomonas oryzae pv. Oryzicola, xoc induced), a phenomenon closely related to activation of the defensive response accompanying the formation of leaf spots. From the physiological aspect, the plant can start physiological metabolism changes locally related to acquired resistance of a system in the process of disease spot generation, on one hand, the disease spot area and surrounding tissues can accumulate defensive hormones such as Salicylic Acid (SA), jasmonic Acid (JA) and the like, wherein SA can enhance early recognition and response capacity [8] of the plant to Xoc infection by regulating and controlling expression of PR (disease-related protein) genes (such as OsPR1 and OsPR 5), on the other hand, the disease spot-induced active oxygen (ROS) can be locally accumulated, although the disease spot is formed by cell programmed death (PCD), the disease spot can be taken as a signal molecule, the activity of a downstream antioxidant enzyme system (such as SOD and POD) can be activated, the capacity of eliminating excessive ROS generated after Xoc infection of the plant is improved, and oxidative damage [9] caused by pathogenic bacteria is r