CN-121991987-A - Soybean RING type E3 ubiquitin ligase gene GmSSE1 and application thereof

Abstract

The invention belongs to the technical field of plant genetics and genetic engineering, and particularly relates to soybean RING type E3 ubiquitin ligase GmSSE1 and application thereof. The invention separates soybean RING type E3 ubiquitin ligase gene GmSSE1 from soybean Williams 82 (Williams 82), and experiments prove that the gene expression is induced by salt stress and can negatively regulate and control the salt tolerance of soybean. Furthermore, by haplotype analysis and phenotype association studies, it was found that there was a significant difference in individual grain weights of the different haplotypes of GmSSE1 (GmSSE 1 Hap1 、GmSSE1 Hap2 and GmSSE1 Hap3 ) in the natural population. The invention not only provides important gene resources for elucidating the salt-tolerant molecular mechanism of plants, but also provides effective molecular targets and technical support for crop salt-tolerant genetic improvement, and has important theoretical significance and application potential.

Inventors

• XIANG FENGNING
• XU JINLONG
• LI SHUO
• Shan Mengqi
• ZHENG XIAOJIAN

Assignees

• 山东大学

Dates

Publication Date

20260508

Application Date

20260206

Claims (10)

1. 1. A soybean RING type E3 ubiquitin ligase gene designated GmSSE1, wherein the soybean RING type E3 ubiquitin ligase gene is selected from the group consisting of: (a1) A nucleotide sequence shown as SEQ ID NO. 1; (a2) A nucleotide sequence which encodes a protein of the same amino acid sequence as the nucleotide sequence of (a 1) but differs in sequence due to the degeneracy of the genetic code; (a3) A nucleotide sequence which has more than or equal to 90% of the identity with the nucleotide sequence shown in (a 1) or (a 2) and codes for a protein with the same or similar functionality; (a4) A nucleotide sequence complementary to any one of (a 1) to (a 3).
2. 2. A protein encoded by the soybean RING type E3 ubiquitin ligase gene of claim 1, wherein the protein is soybean RING type E3 ubiquitin ligase.
3. 3. A recombinant expression vector, transgenic cell line, host bacterium or transgenic plant comprising the soybean RING type E3 ubiquitin ligase gene of claim 1.
4. 4. The recombinant expression vector, transgenic cell line, host cell, or transgenic plant of claim 3, wherein the recombinant expression vector is operably linked to an expression vector, which can be any one or more of a viral vector, a plasmid, a phagemid, a cosmid, or an artificial chromosome, by a soybean RING type E3 ubiquitin ligase gene; Wherein the plant cells are crop cells and the crop is a dicotyledonous crop, wherein leguminous plants, particularly plants of the genus Glycine, are preferred, most preferably Glycine max; wherein the host bacteria are bacteria, fungi and actinomycetes; the transgenic plant is a transgenic crop, further preferably a dicotyledonous crop, wherein leguminous plants, especially plants of the genus Glycine, are preferred, most preferably Glycine max.
5. 5. Use of the soybean RING type E3 ubiquitin ligase gene of claim 1, the soybean RING type E3 ubiquitin ligase of claim 2, the recombinant expression vector of any one of claims 3-4, a transgenic cell line, a host bacterium, or a transgenic plant in any one or more of: (b1) Regulating and controlling the salt tolerance and/or high yield performance of plants; (b2) Improving and/or growing salt tolerant and/or high yielding plants; (b3) Screening or identifying salt tolerant and/or high yielding plants.
6. 6. The use according to claim 5, wherein in (b 1) the regulation of plant salt tolerance is characterized in that the soybean RING type E3 ubiquitin ligase gene is inhibited from being expressed in soybean, and the fresh weight of the expressed soybean in a salt environment is inhibited from being increased compared with a control group.
7. 7. The use according to claim 5, wherein in (b 2) the improvement and/or cultivation of plants is embodied as improvement and cultivation of a plant variety having salt tolerance, said plants being crops, further preferably dicotyledonous crops, wherein leguminous plants, especially plants of the genus Glycine, are preferred, most preferably Glycine max.
8. 8. A method for improving and growing salt tolerant plants, which comprises increasing the expression level and/or activity of the GmSSE gene according to claim 1 endogenous to the plant or allowing plants not containing GmSSE1 gene to express the GmSSE gene and/or the protein according to claim 2.
9. 9. The method according to claim 8, wherein the plant is any plant at any stage of development, in particular the plant is a crop, further preferably a dicotyledonous crop, wherein plants of the family leguminosae, in particular plants of the genus glycine, are preferred, most preferably soybean.
10. 10. A method for screening salt-resistant plants, which comprises detecting the transcript level of GmSSE gene of the plant or detecting the expression level or activity of the above protein.

Description

Soybean RING type E3 ubiquitin ligase gene GmSSE1 and application thereof Technical Field The invention belongs to the technical field of plant genetics and genetic engineering, and particularly relates to a soybean RING type E3 ubiquitin ligase gene GmSSE and application thereof. Background The disclosure of this background section is only intended to increase the understanding of the general background of the invention and is not necessarily to be construed as an admission or any form of suggestion that this information forms the prior art already known to those of ordinary skill in the art. Soil salinization severely affects crop growth and results in crop yield reduction, and has become a key factor limiting global agricultural production and sustainable development. Plant growth is affected by biotic and abiotic stresses, and its viability under stress is largely dependent on the plasticity of the proteome. The ubiquitination modification is an important protein posttranslational modification and plays an important role in the growth and development of eukaryotes and responding to various abiotic stresses. In complex regulatory networks where plants cope with environmental stresses, post-translational modification of proteins plays a vital role. The ubiquitin-proteasome pathway is used as a highly specific protein degradation mechanism, and can timely adjust the abundance of certain specific proteins, thereby playing a vital role in the processes of sensing external signals, regulating growth and development and responding to abiotic stress of plants. The core step of this pathway involves a cascade of ubiquitin activating enzyme (E1), ubiquitin binding enzyme (E2) and ubiquitin ligase (E3), which ultimately covalently labels ubiquitin molecules onto the substrate protein, leading to its degradation by the 26S proteasome. In this system, the E3 ubiquitin ligase is a key factor in determining ubiquitination targeting and efficiency due to its ability to specifically recognize substrates. The plant genome encodes a vast number of E3 ligases, largely classified into HECT, RING, U-box and CRLs, which are deeply involved in various stress responses by mediating ubiquitination degradation of specific substrates. However, there are still few reports on their role in plant salt tolerance. Disclosure of Invention Aiming at the defects existing in the prior art, the invention aims to provide a soybean RING type E3 ubiquitin ligase gene GmSSE1 and application thereof. Specifically, the invention separates soybean RING type E3 ubiquitin ligase gene GmSSE1 from soybean Williams 82 (Williams 82), and experiments prove that the gene expression is induced by salt stress and can negatively regulate and control the salt tolerance of soybean. Furthermore, by haplotype analysis and phenotype association studies, it was found that there was a significant difference in the individual weights of the different haplotypes of GmSSE1 (GmSSE 1 Hap1、GmSSE1Hap2 and GmSSE1 Hap3) in the natural population, with the individual weight of GmSSE1 Hap2 being significantly higher than GmSSE1 Hap3, while being higher than GmSSE1 Hap1. Based on the above results, the present invention has been completed. In order to achieve the technical purpose, the technical scheme provided by the invention is as follows: In a first aspect of the present invention, there is provided a soybean RING type E3 ubiquitin ligase gene designated GmSSE1, said soybean RING type E3 ubiquitin ligase gene selected from the group consisting of: (a1) A nucleotide sequence shown as SEQ ID NO. 1; (a2) A nucleotide sequence which encodes a protein of the same amino acid sequence as the nucleotide sequence of (a 1) but differs in sequence due to the degeneracy of the genetic code; (a3) A nucleotide sequence which has more than or equal to 90% of the identity with the nucleotide sequence shown in (a 1) or (a 2) and codes for a protein with the same or similar functionality; (a4) A nucleotide sequence complementary to any one of (a 1) to (a 3). In a second aspect of the present invention, there is provided a protein encoded by the above soybean RING type E3 ubiquitin ligase gene, in particular soybean RING type E3 ubiquitin ligase. In a third aspect of the invention, there is provided a recombinant expression vector, transgenic cell line, host bacterium or transgenic plant. In a fourth aspect of the present invention, there is provided the use of the above soybean RING type E3 ubiquitin ligase gene, soybean RING type E3 ubiquitin ligase, recombinant expression vector comprising the above soybean RING type E3 ubiquitin ligase gene, transgenic cell line, host bacterium or transgenic plant in any one or more of the following: (b1) Regulating and controlling the salt tolerance and/or high yield performance of plants; (b2) Improving and/or growing salt tolerant and/or high yielding plants; (b3) Screening or identifying salt tolerant and/or high yielding plants. In the present inven