CN-121991988-A - Gene ZmRCHY for enhancing salt tolerance of plants and application thereof

CN121991988ACN 121991988 ACN121991988 ACN 121991988ACN-121991988-A

Abstract

The invention relates to a gene ZmRCHY for enhancing plant salt tolerance and application thereof, belonging to the technical field of plant gene breeding, wherein the nucleotide sequence of the gene ZmRCHY is shown as SEQ ID NO.1, and the amino acid sequence of protein encoded by the gene is shown as SEQ ID NO.2, so that the salt tolerance of plants can be enhanced by over-expression of the gene ZmRCHY 3. According to the invention, through a gene overexpression experiment, it is verified that the salt tolerance of a corn plant can be enhanced by the overexpression of the gene ZmRCHY, a novel salt-tolerant material is obtained based on the novel function development of the corn ZmRCHY gene, a genetic basis is provided for the breeding of a corn salt-tolerant variety, and an important theoretical basis is provided for creating a novel plant germplasm and plant molecular breeding.

Inventors

PENG XIAOJIAN
SU ANQI
YANG YUXIAN
RAO WENHUI
JIANG HAIYANG
MA QING
ZHAO YANG
WEI XIAO
XIE SHANSHAN

Assignees

安徽农业大学

Dates

Publication Date: 20260508
Application Date: 20260225

Claims (7)

1. A gene ZmRCHY is characterized in that the nucleotide sequence of the gene ZmRCHY3 is shown as SEQ ID NO.1, and the amino acid sequence of the protein encoded by the gene is shown as SEQ ID NO. 2.
2. Use of the gene ZmRCHY according to claim 1 for enhancing salt tolerance in plants.
3. The method according to claim 2, wherein said gene ZmRCHY positively regulates the salt tolerance of a plant.
4. The method of claim 3, wherein gene ZmRCHY is overexpressed to enhance salt tolerance in the plant.
5. The method of claim 3, wherein the deletion of gene ZmRCHY results in a decrease in salt tolerance of the plant.
6. The method according to claim 2, wherein the plant variety with high salt tolerance is obtained by using enhancer knock-in technology or screening haplotypes with high expression level from natural ecology during plant breeding.
7. The method according to claim 2, wherein the plant is any one of maize, arabidopsis and rice.

Description

Gene ZmRCHY for enhancing salt tolerance of plants and application thereof Technical Field The invention belongs to the technical field of plant gene breeding, and particularly relates to a gene ZmRCHY for enhancing plant salt tolerance and application thereof. Background Salt stress is one of the key abiotic stresses restricting global agricultural production, corn is one of three world food crops, and the growth, development and yield formation of the corn are extremely sensitive to salt. Salt stress not only inhibits the vegetative growth and reproductive development of corn, but also reduces photosynthetic efficiency and interferes with physiological metabolism, ultimately resulting in serious yield reduction. Previous studies have found that ubiquitination modifications play a critical role in plant response to salt stress. And E3 ubiquitin ligase is in the core position in ubiquitination modification and plays a role of a key regulator. The ubiquitination process requires sequential catalysis of E1 (activating enzyme), E2 (binding enzyme) and E3 (ligase). The E1 and E2 species are relatively few and are mainly responsible for the activation and delivery of ubiquitin molecules. E3 is responsible for precisely recognizing specific target proteins (substrates), and the ubiquitin monomers are linked to the target proteins, which are the "commander" and "end effector" of the ubiquitin-proteasome system. Among all kinds of E3 ubiquitin ligases, there is a special type called RCHY type E3 ubiquitin ligases, which plays an important role in targeting regulatory key proteins for degradation, and the prior art discloses the results of studies on RCHY type E3 ubiquitin ligases in humans (RCHY 1, alias PIRH 2) and mice (Rchy 1), but the studies on RCHY type E3 ubiquitin ligases in plants are extremely limited, only involving studies based on histologic data, not involving deep molecular functional studies. In contrast, the invention provides a gene ZmRCHY for enhancing salt tolerance of plants and application thereof. Disclosure of Invention The present invention aims to solve the above problems and provide a gene ZmRCHY for enhancing salt tolerance of plants and an application thereof. The invention realizes the above purpose through the following technical scheme: the invention provides a gene ZmRCHY, the nucleotide sequence of the gene ZmRCHY is shown as SEQ ID NO.1, and the amino acid sequence of the protein encoded by the gene is shown as SEQ ID NO. 2. The invention also provides an application of the gene ZmRCHY in enhancing the salt tolerance of plants. As a further optimization scheme of the invention, the gene ZmRCHY positively regulates the salt tolerance of the plant. As a further optimization of the invention, gene ZmRCHY is over-expressed to enhance the salt tolerance of the plant. As a further optimization scheme of the invention, the salt tolerance of the plant is reduced due to the fact that the gene ZmRCHY is in a function deficiency mode. As a further optimization scheme of the invention, the enhancer knock-in technology or the haplotype with high expression level is selected from natural ecology in the plant breeding process to obtain the plant variety with high salt tolerance. As a further optimization scheme of the invention, the plant is any one of corn, arabidopsis and rice. The invention has the beneficial effects that: The invention discovers a gene ZmRCHY for enhancing the salt tolerance of a plant, and verifies that the over-expression of the gene ZmRCHY can enhance the salt tolerance of a corn plant through a gene knockout experiment and a gene over-expression experiment, and the method is characterized in that under salt stress, the growth state of a ZmRCHY3 over-expression plant is obviously superior to that of a wild type, the quality of an overground part is obviously higher than that of the wild type, the Na + content of the overground part is obviously lower than that of the wild type, the Pro content of the overground part is obviously higher than that of the wild type, a novel salt-tolerant material can be developed and obtained based on the novel function of the corn ZmRCHY3 gene, a genetic basis is provided for the breeding of a corn salt-tolerant variety, and an important theoretical basis is provided for creating a novel germplasm of the plant and molecular breeding of the plant. Drawings FIG. 1 is a ZmRCHY CDS full-length amplification electrophoretogram [ note: lane 1 is DNA MARKER (250 bp-2000 bp), lanes 2,3 are ZmRCHY gene fragments ]; FIG. 2 shows the mutation sites ZmRCHY, crispr-1 and ZmRCHY, 3 and crispr-2; FIG. 3 shows the salt treatment phenotype of KN5585 and ZmRCHY3 crispr plants; FIG. 4 is a statistical result of the quality of the aerial parts of KN5585 and ZmRCHY 3. 3 crispr plants; FIG. 5 is a graph showing the statistical results of Na + content of aerial parts of KN5585 and ZmRCHY 3. 3 crispr plants; FIG. 6 is a graph showing the statistics of proline cont