CN-121991989-A - UDP sugar pyrophosphorylase CpUSP gene and application thereof in promotion of microalgae harvesting and crushing

Abstract

The invention relates to a UDP sugar pyrophosphorylase CpUSP gene and application thereof in promotion of microalgae harvesting and crushing in the field of bioengineering, the nucleotide sequence of the CpUSP gene is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2. After the UDP-sugar pyrophosphorylase of the chlorella pyrenoidosa is used for over-expression in the chlamydomonas, the cell morphology is changed, the harvesting and crushing efficiency of the chlamydomonas is obviously improved, and the invention provides a new technical scheme for reducing the harvesting and cell crushing cost of the microalgae.

Inventors

• WANG SHIKAI
• Huang Shuaili
• LI HUIXIN
• LI ZHIFENG

Assignees

• 扬州大学

Dates

Publication Date

20260508

Application Date

20260318

Claims (5)

1. 1. The UDP-sugar pyrophosphorylase CpUSP gene is characterized in that the nucleotide sequence of the CpUSP gene is shown as SEQ ID NO. 1, and the amino acid sequence is shown as SEQ ID NO. 2.
2. 2. The application of the UDP-sugar pyrophosphorylase CpUSP gene in promotion of microalgae harvesting and crushing is characterized in that the nucleotide sequence of the CpUSP gene is shown as SEQ ID NO. 1, the amino acid sequence is shown as SEQ ID NO. 2, and the UDP-sugar pyrophosphorylase CpUSP gene promotes microalgae cell harvesting and crushing by regulating cell wall characteristics.
3. 3. The use of UDP sugar pyrophosphorylase CpUSP gene of claim 2 for promoting harvesting and disruption of microalgae comprising chlorella pyrenoidosa, chlorella ellipsoidea, chlorella vulgaris, chlamydomonas reinhardtii, scenedesmus, haematococcus pluvialis or nannochloropsis.
4. 4. Use of the UDP sugar pyrophosphorylase CpUSP gene of claim 2 or 3 to promote microalgae harvesting and disruption, wherein microalgae harvesting and disruption is promoted by overexpression of the CpUSP gene in the corresponding microalgae.
5. 5. The use of UDP sugar pyrophosphorylase CpUSP gene of claim 4 for promoting microalgae harvesting and disruption, wherein the over-expression method comprises one of electroporation, gene gun, agrobacterium-mediated method, and CRISPR/Cas 9-mediated gene editing delivery method.

Description

UDP sugar pyrophosphorylase CpUSP gene and application thereof in promotion of microalgae harvesting and crushing Technical Field The invention relates to the fields of biology and bioengineering, in particular to a UDP sugar pyrophosphorylase CpUSP gene and application thereof in promotion of microalgae harvesting and crushing. Background Microalgae are microorganisms capable of photosynthesis, can accumulate abundant nutritional ingredients such as protein, grease, polysaccharide, pigment and the like through photosynthesis, and have wide market prospects in the fields of dietary supplements, foods/feeds, medicines, cosmetics, biofuels and the like. In the process of microalgae high-valued utilization, harvesting biomass from a culture medium is a great challenge, and due to the micro-algae cells with small size, low density and good dispersibility, the energy consumption and equipment cost in the harvesting process are high, and research shows that the harvesting cost can account for 30% of the total production cost. In addition to cell harvesting, cell disruption is another bottleneck problem in the development of microalgae products. Because the cell walls of most economic algae have complex compositions and compact structures, the conventional cell disruption process is faced with the problems of high energy consumption, high cost and the like, for example, the energy consumption range of a mechanical disruption method is up to 100-504 MJ/kg microalgae dry weight. In addition to affecting product extraction, the cell wall also limits its availability to humans and animals when microalgae cells are used as a whole as a nutraceutical or health food additive. As two major bottlenecks in development of microalgae industry, challenges of cell harvesting and cell disruption processes are directly related to characteristics of microalgae cell walls, cell harvesting and disruption are expected to be promoted by regulating the characteristics of the cell walls, and a great deal of research has been conducted on promoting cell harvesting and disruption by changing culture conditions, and common induction strategies include high-temperature treatment (patent CN 116042404A), high CO 2, nitrogen deficiency stress and the like, but the above strategies can promote cell harvesting or disruption, but also affect growth and product accumulation of microalgae cells. The composition and structure of the microalgae cell wall can be directionally modified to serve as a new way for reducing cell disruption, harvesting and cost. However, currently, cell harvesting and cell disruption studies are focused more on process development, and less on microalgae cell wall regulation. Disclosure of Invention The invention aims to solve the problems of high energy consumption and high cost in the process of cell harvesting and crushing cost faced by the current microalgae industry, and provides a gene CpUSP capable of promoting microalgae cell harvesting and crushing by regulating cell wall characteristics and application thereof. The inventor creatively discovers that the over-expression of USP genes in chlorella can change cell morphology, more importantly can effectively improve cell harvesting and crushing efficiency, and further verifies in Chlamydomonas reinhardtii to prove that CpUSP genes have certain universality. The technical scheme adopted by the invention is as follows: a UDP sugar pyrophosphorylase CpUSP gene, the nucleotide sequence of the CpUSP gene is shown as SEQ ID NO.1, and the amino acid sequence is shown as SEQ ID NO. 2. The nucleotide sequence of the UDP-sugar pyrophosphorylase CpUSP gene is shown as SEQ ID NO. 1, the amino acid sequence of the CpUSP gene is shown as SEQ ID NO. 2, and the UDP-sugar pyrophosphorylase CpUSP gene promotes microalgae cell harvesting and crushing by regulating cell wall characteristics. Use of the UDP sugar pyrophosphorylase CpUSP gene to facilitate microalgae harvesting and disruption by over-expressing the CpUSP gene in the corresponding microalgae. UDP sugar pyrophosphorylase CpUSP gene and application thereof in promoting harvesting and crushing of microalgae, wherein the microalgae comprise Chlorella pyrenoidosa, chlorella ellipsoidea, chlorella vulgaris, chlamydomonas reinhardtii, scenedesmus, haematococcus pluvialis and nannochloropsis. UDP sugar pyrophosphorylase CpUSP gene and its use in promoting microalgae harvesting and disruption, including electroporation, gene gun, agrobacterium-mediated and CRISPR/Cas 9-mediated gene editing delivery. Compared with the prior art, the invention has the innovation points and the advancement that: (1) The CpUSP gene provided by the invention can promote the collection and the crushing of microalgae cells. (2) The CpUSP gene provided by the invention can promote cell harvesting and breaking, and can not influence the growth of microalgae cells. . (3) The CpUSP gene is utilized to change the composition and structural characteristics of t