CN-121991996-A - By using industrial waste gas CO2Construction method of photosynthetic engineering bacteria for producing succinic acid
Abstract
The invention discloses a construction method of photosynthetic engineering bacteria for producing succinic acid by utilizing industrial waste gas CO 2 , which relates to the technical field of biology, and comprises the following steps of S1, selecting a culture strain, namely selecting a natural microorganism with photosynthetic carbon fixing capability, such as blue algae or photosynthetic bacteria, S2, carrying out target analysis on genes of the strain, judging genes for producing succinic acid in strain genes, strengthening or introducing genes for producing succinic acid through gene combination engineering, optimizing carbon flow direction succinic acid, introducing genes with acid resistance, and improving acid resistance of the strain, and S3, culturing the photosynthetic engineering bacteria.
Inventors
- WANG CHAO
Assignees
- 北京碳和新材未来科技有限公司
Dates
- Publication Date
- 20260508
- Application Date
- 20260226
Claims (8)
- 1. The construction method of the photosynthetic engineering bacteria for producing succinic acid by utilizing industrial waste gas CO 2 is characterized by comprising the following steps: S1, selecting culture strains, namely selecting microorganisms with photosynthetic carbon fixing capability, such as blue algae or photosynthetic bacteria; S2, gene editing, namely judging a gene for producing succinic acid by CO 2 in a strain gene by carrying out target analysis on the strain gene, strengthening or introducing the gene for producing succinic acid by gene combination engineering, optimizing the carbon flow direction of succinic acid, and introducing a gene with acid-resistant property for improving the acid-resistant property of the strain; S3, culturing the photosynthetic engineering bacteria, namely culturing the bacteria in the S2, and simultaneously introducing industrial waste gas in the culture process for screening the photosynthetic engineering bacteria with acid-resistant property and stronger succinic acid production; and S4, culturing the photosynthetic engineering bacteria screened in the step S3 at high density.
- 2. The method for constructing photosynthetic engineering bacteria for producing succinic acid by utilizing industrial waste gas CO 2 as claimed in claim 1, wherein the genes intensified or introduced in the gene editing can be phosphoenolpyruvate carboxylase, succinate dehydrogenase, etc.
- 3. The construction method of photosynthetic engineering bacteria for producing succinic acid by utilizing industrial waste gas CO 2 as claimed in claim 2, wherein the acid-resistant gene introduced in the gene editing is required to have the capability of resisting sulfuric acid and nitric acid, and is used for resisting the acidic environment generated in the industrial waste gas dissolved water.
- 4. The construction method of photosynthetic engineering bacteria for producing succinic acid by utilizing industrial waste gas CO 2 as claimed in claim 3, wherein the rapid screening of genes with CO 2 for producing succinic acid and acid resistance can be realized by querying a database of known ligand-target point relation in the process of gene editing.
- 5. The method for constructing photosynthetic engineering bacteria for succinic acid production by utilizing industrial waste gas CO 2 as claimed in claim 4, wherein the acid-fast characteristic genes introduced in the gene editing process can be a plurality of different gene sequences, and can resist different acids respectively.
- 6. The construction method of photosynthetic engineering bacteria for succinic acid production by using industrial waste gas CO 2 as claimed in claim 5, wherein the precise editing of genes is performed by using CRISPR-Cas9 or homologous recombination technology in gene editing.
- 7. The construction method of photosynthetic engineering bacteria for producing succinic acid by utilizing industrial waste gas CO 2 as claimed in claim 1, wherein the concentration of succinic acid and the pH value of the culture solution are monitored in real time in the culture process of the photosynthetic engineering bacteria.
- 8. The construction method of photosynthetic engineering bacteria for producing succinic acid by using industrial waste gas CO 2 as claimed in claim 1, wherein a flat plate type reactor is used in the cultivation and high-density cultivation processes of the photosynthetic engineering bacteria.
Description
Construction method of photosynthetic engineering bacteria for producing succinic acid by using industrial waste gas CO 2 Technical Field The invention relates to the technical field of biology, in particular to a construction method of photosynthetic engineering bacteria for producing succinic acid by utilizing industrial waste gas CO 2. Background In the industrial process, a large amount of CO 2 is generated, and in order to reduce carbon emissions, the treatment of CO 2 is performed in various manners in the prior art, including but not limited to various physical manners, chemical manners and biological manners, and the biological transformation method has become a research hot spot due to environmental protection and sustainability advantages. In the prior art, the synthesis of succinic acid by using light energy and CO 2 through genetically modified photosynthetic engineering bacteria (such as blue algae) is a very mature technology, and CO 2 can be effectively fixed, but industrial waste gas contains a large amount of CO 2 and a certain amount of SO 2, NO 2 and other gases, when the photosynthetic engineering bacteria are used for fixing CO 2 in the industrial waste gas, the rest waste gas in the industrial waste gas needs to be effectively removed, SO that the treatment cost of the industrial waste gas CO 2 is greatly increased, and therefore, the construction method of the photosynthetic engineering bacteria for producing succinic acid by using the industrial waste gas CO 2 is proposed. Disclosure of Invention The invention aims to provide a construction method of photosynthetic engineering bacteria for producing succinic acid by utilizing industrial waste gas CO 2. In order to achieve the purpose, the invention provides the following technical scheme that the construction method of the photosynthetic engineering bacteria for producing succinic acid by utilizing industrial waste gas CO 2 comprises the following steps: S1, selecting culture strains, namely selecting microorganisms with photosynthetic carbon fixing capability, such as blue algae or photosynthetic bacteria; S2, gene editing, namely judging a gene for producing succinic acid by CO 2 in a strain gene by carrying out target analysis on the strain gene, strengthening or introducing the gene for producing succinic acid by gene combination engineering, optimizing the carbon flow direction of succinic acid, and introducing a gene with acid-resistant property for improving the acid-resistant property of the strain; S3, culturing the photosynthetic engineering bacteria, namely culturing the bacteria in the S2, and simultaneously introducing industrial waste gas in the culture process for screening the photosynthetic engineering bacteria with acid-resistant property and stronger succinic acid production; and S4, culturing the photosynthetic engineering bacteria screened in the step S3 at high density. As a further aspect of the present invention, the gene to be enhanced or introduced in the gene editing may be phosphoenolpyruvate carboxylase, succinate dehydrogenase or the like. As a further aspect of the present invention, the acid-resistant gene introduced in the gene editing is required to have resistance to sulfuric acid and nitric acid for resisting the acidic environment generated in the industrial waste water. As a further scheme of the invention, in the process of gene editing, the rapid screening of genes with CO 2 for succinic acid production and acid resistance can be realized by querying a known ligand-target point relation database. As a further aspect of the present invention, the acid-fast characteristic gene introduced in the gene editing process may be a plurality of different gene sequences, and may be resistant to different acids, respectively. As a further aspect of the present invention, precise editing of genes is performed by using CRISPR-Cas9 or homologous recombination technology in gene editing. As a further scheme of the invention, the concentration of the succinic acid and the pH value of the culture solution are required to be monitored in real time in the culture process of the photosynthetic engineering bacteria. As a further scheme of the invention, a flat plate type reactor is used in the culture and high-density culture processes of the photosynthetic engineering bacteria. By adopting the technical scheme, compared with the prior art, the invention has the beneficial effects that: 1. The acid resistance is obviously improved, the method can be suitable for different SO 2 and NO 2 concentrations in industrial waste gas, and the photosynthetic engineering bacteria are healthy in state growth; 2. The treatment effect on CO 2 in industrial waste gas is strong, and photosynthesis carbon fixation can be effectively carried out. Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the