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CN-121991997-A - FvSup1 protein and application of encoding gene thereof in regulating and controlling pathogenicity of fusarium pseudoverticillium

CN121991997ACN 121991997 ACN121991997 ACN 121991997ACN-121991997-A

Abstract

The invention discloses FvSup protein and application of a coding gene thereof in regulating and controlling pathogenicity of fusarium verticillium, and belongs to the technical field of biology. The invention firstly researches and proves the novel function of the Fusarium verticillium unknown functional protein FvSup and the encoding gene FvSup1 thereof. The invention constructs FvSup gene knockout vector, introduces the vector into fusarium pseudoverticillatum protoplast to obtain knockout mutant delta FvSup1, constructs gene complement vector, introduces the vector into delta FvSup1 protoplast to obtain complement mutant delta FvSup1-C. Pathogenicity assays showed that the knock-out mutant Δ FvSup had significantly reduced pathogenicity, and that the pathogenicity of the boost mutant Δ FvSup1-C was able to recover to wild-type levels. The test proves that FvSup is a pathogenic related gene of the fusarium verticillium, and provides a new target for preventing and controlling plant diseases caused by the fusarium verticillium.

Inventors

  • HAN CHAO
  • WANG SHENGNAN

Assignees

  • 山东农业大学

Dates

Publication Date
20260508
Application Date
20260116

Claims (10)

  1. Use of fvsupp 1 protein for modulating pathogenicity of fusarium pseudoverticillium, said FvSup protein being a protein as shown in (A1) or (A2) below: (A1) A protein with an amino acid sequence shown as SEQ ID NO. 3; (A2) And (3) a protein with the same function, wherein the amino acid sequence shown in SEQ ID NO.1 is subjected to substitution and/or deletion and/or addition of one or more amino acid residues.
  2. 2. Use according to claim 1, characterized in that the pathogenicity of fusarium verticillium is reduced by reducing the expression level and/or activity of FvSup a protein.
  3. Use of fvsupp 1 protein as a target in (1) or (2) as follows: (1) Developing a medicament for preventing and treating plant diseases; (2) Cultivating plant disease-resistant varieties of plants.
  4. 4. The use according to claim 3, wherein the plant disease is caused by fusarium verticillium.
  5. 5. Use of FvSup gene for modulating virulence of fusarium verticillium, said FvSup gene being a nucleic acid molecule as set forth in (i) or (ii) or (iii): (i) A nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 2; (ii) A nucleic acid molecule other than (i) encoding the amino acid sequence shown in SEQ ID NO. 1; (iii) A nucleic acid molecule which has 90% or more identity to the nucleic acid molecule defined in i) or ii) and which encodes a protein functionally equivalent to the protein shown in SEQ ID NO. 1.
  6. 6. The use according to claim 5, wherein the pathogenicity of fusarium verticillium is reduced by knocking out or silencing FvSup gene, or inhibiting FvSup1 gene transcription.
  7. 7. Use of a substance that modulates FvSup gene expression or a substance that modulates FvSup protein activity and/or content in at least one of the following (1) - (3): (1) Regulating and controlling pathogenicity of fusarium verticillium; (2) Preparing a medicament for preventing and treating plant diseases; (3) Cultivating plant disease-resistant varieties of plants.
  8. 8. The use according to claim 7, wherein the plant disease is caused by fusarium verticillium.
  9. 9. The use according to claim 7, wherein the substance regulating FvSup gene expression or the substance regulating FvSup protein activity and/or content is any of the following: e1 A nucleic acid molecule that inhibits or reduces or silences FvSup a gene expression; e2 An expression cassette comprising e 1) said nucleic acid molecule; e3 A recombinant vector comprising e 1) said nucleic acid molecule, or a recombinant vector comprising e 2) said expression cassette; e4 A recombinant microorganism comprising e 1) said nucleic acid molecule, or a recombinant microorganism comprising e 2) said expression cassette, or a recombinant microorganism comprising e 3) said recombinant vector.
  10. Use of fvsupp 1 protein in at least one of the following (1) - (4): (1) Degrading plant cell walls; (2) Degrading xylan; (3) Preparing a product for degrading plant cell walls; (4) Preparing a xylan-degrading product; The FvSup protein is a protein shown in any one of the following (A1) - (A3): (A1) A protein with an amino acid sequence shown as SEQ ID NO. 3; (A2) A protein obtained by ligating the N-terminal and/or C-terminal of the protein defined in (A1) with a protein tag; (A3) And (3) a protein with the same function, wherein the amino acid sequence shown in SEQ ID NO.1 is subjected to substitution and/or deletion and/or addition of one or more amino acid residues.

Description

FvSup1 protein and application of encoding gene thereof in regulating and controlling pathogenicity of fusarium pseudoverticillium Technical Field The invention relates to the technical field of biology, in particular to FvSup protein and application of a coding gene thereof in regulating and controlling pathogenicity of fusarium pseudoverticillium. Background Fusarium (Fusarium) is taken as a globally distributed plant pathogenic fungus, can be widely used for infecting a plurality of important crops such as wheat, corn, tomatoes and the like, induces destructive diseases such as scab, fusarium wilt, stem rot and the like, seriously threatens the global grain safety, and needs to establish a high-efficiency and feasible prevention and control system. At present, fusarium diseases are prevented and controlled by taking chemical control as a main means, but long-term large-scale application of chemical pesticides not only leads to enhancement of pathogen resistance and attenuation of control effect, but also forces continuous increase of dosage, and also causes serious environmental pollution and agricultural product pesticide residue problems, thus forming double threats to ecological safety and human health. Therefore, the research and development of novel green control agents and the cultivation of disease-resistant crop varieties have become the core development direction of fusarium disease control, and the key of technological breakthroughs is to precisely excavate the core pathogenic targets of germs. Although the whole genome sequencing work of various pathogenic fusarium has been successfully completed at present, the pathogenic related genes with definite functions in the genome are still relatively deficient. Fusarium verticillium (Fusarium verticillioides) is a common pathogenic fungus of the genus Fusarium and can cause a variety of transmissible diseases of food crops, particularly corn. The whole genome sequencing work of Fusarium verticillium (Fusarium verticillioides) has been completed, but the genome of this strain contains 14,179 protein-encoding genes, and about 40% -60% of genes are labeled "hypothetical protein (hypothetical protein)" or "uncharacterized protein (uncharacterised protein) according to the annotation statistics of databases such as NCBI and JGI Mycocosm. Therefore, the pathogenic related genes of the fusarium pseudoverticillatum (Fusarium verticillioides) are fully excavated and the functional research is carried out, the pathogenic molecular mechanism of the fusarium pseudoverticillatum is fully known, and a theoretical basis is provided for the prevention and control of the fusarium pseudoverticillatum. Disclosure of Invention Aiming at the prior art, the invention aims to provide FvSup protein and application of a coding gene thereof in regulating and controlling pathogenicity of fusarium pseudoverticillium. In order to achieve the above purpose, the invention adopts the following technical scheme: In a first aspect of the invention, there is provided the use of FvSup a protein for modulating pathogenicity of fusarium graminearum, said FvSup a protein being a protein as shown in (A1) or (A2) below: (A1) A protein with an amino acid sequence shown as SEQ ID NO. 3; (A2) And (3) a protein with the same function, wherein the amino acid sequence shown in SEQ ID NO.1 is subjected to substitution and/or deletion and/or addition of one or more amino acid residues. In the above application, the pathogenicity of fusarium graminearum is reduced by reducing the expression level and/or activity of FvSup protein. The invention discovers that FvSup protein is a novel key pathogenic factor of the fusarium pseudoverticillatum and can regulate and control the pathogenicity of the fusarium pseudoverticillatum for the first time. By taking FvSup protein as a target, a novel medicine for preventing and treating plant diseases caused by fusarium verticillium can be developed. In a second aspect of the present invention, there is provided the use of the FvSup protein as described above as a target in (1) or (2) as follows: (1) Developing a medicament for preventing and treating plant diseases; (2) Cultivating plant disease-resistant varieties of plants. In the above application, the plant disease is caused by fusarium verticillium. In a third aspect of the invention there is provided the use of FvSup a gene for modulating virulence of fusarium verticillium, said FvSup a gene being a nucleic acid molecule as set out in (i) or (ii) or (iii): (i) A nucleic acid molecule with a nucleotide sequence shown as SEQ ID NO. 2; (ii) A nucleic acid molecule other than (i) encoding the amino acid sequence shown in SEQ ID NO. 1; (iii) A nucleic acid molecule which has 90% or more identity to the nucleic acid molecule defined in i) or ii) and which encodes a protein functionally equivalent to the protein shown in SEQ ID NO. 1. In the above applications, the nucleic acid molecule may be DNA, such as cDNA, g