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CN-121992002-A - Soybean GmSMS protein and application of encoding gene thereof in regulation and control of grain weight of soybean seeds

CN121992002ACN 121992002 ACN121992002 ACN 121992002ACN-121992002-A

Abstract

The invention provides application of soybean GmSMS protein and a coding gene thereof in regulating and controlling the grain weight and grain size of soybean seeds. The invention proves the function of soybean GmSMS6 in regulating and controlling the grain weight of soybean through CRSIPR-Cas9 system for the first time. GmSMS6 is a negative regulation gene of soybean grain weight, and knocking out the gene can obviously improve soybean grain weight, and the over-expression plant has obviously reduced hundred grain weight compared with wild type. The phenotype of the transgenic plant shows that GmSMS negative regulation of the soybean grain weight can be used for high-yield soybean breeding. The invention provides important gene resources and theoretical guidance for soybean high-yield molecular breeding and germplasm innovation.

Inventors

  • HE CHAOYING
  • LI BINGBING
  • WANG YAN

Assignees

  • 中国科学院植物研究所

Dates

Publication Date
20260508
Application Date
20241106

Claims (10)

  1. 1. Use of a biomaterial, characterized in that the use is any one of the following: A1 The application of the biological material in regulating and controlling the plant grain character and/or preparing a product for regulating and controlling the plant grain character, A2 Use of said biological material in plant breeding and/or in the preparation of plant breeding products; the biological material is protein, a substance for regulating and controlling the expression of the encoding gene of the protein or a substance for regulating and controlling the content of the protein; The protein is GmSMS and is any one of the following: B1 Amino acid sequence is a protein shown in sequence 2, B2 A protein which is obtained by substituting and/or deleting and/or adding amino acid residues of the protein B1), has more than 80 percent of identity with the protein B1) and can regulate the properties of plant seeds, B3 Fusion proteins obtained by ligating the N-terminal or/and C-terminal of B1) or B2) with a protein tag.
  2. 2. The use according to claim 1, wherein the protein is derived from soybean.
  3. 3. The use according to claim 1 or 2, wherein the substance is any of the following: C1 An RNA molecule that inhibits or reduces or down-regulates the expression of a gene encoding the protein according to claim 1 or 2 or an RNA molecule that inhibits or reduces or down-regulates the activity or content of the protein; C2 Expression of C1) the gene encoding the RNA molecule; C3 An expression cassette containing the gene of C2); C4 A recombinant vector comprising the gene of C2) or a recombinant vector comprising the expression cassette of C3); c5 A recombinant microorganism containing the gene of C2) or a recombinant microorganism containing the expression cassette of C3) or a recombinant microorganism containing the recombinant vector of C4); C6 A transgenic plant cell line containing the gene of C2) or a transgenic plant cell line containing the expression cassette of C3) or a transgenic plant cell line containing the recombinant vector of C4); c7 A transgenic plant tissue containing the gene of C2) or a transgenic plant tissue containing the expression cassette of C3) or a transgenic plant tissue containing the recombinant vector of C4); c8 A transgenic plant organ containing the gene of C2) or a transgenic plant organ containing the expression cassette of C3) or a transgenic plant organ containing the recombinant vector of C4); c9 A nucleic acid molecule encoding a protein as claimed in claim 1 or 2; C10 An expression cassette comprising C9) said nucleic acid molecule; C11 A recombinant vector comprising C9) said nucleic acid molecule or a recombinant vector comprising C10) said expression cassette; C12 A recombinant microorganism comprising C9) said nucleic acid molecule or a recombinant microorganism comprising C10) said expression cassette or a recombinant microorganism comprising C11) said recombinant vector; c13 A transgenic plant cell line comprising C9) said nucleic acid molecule or a transgenic plant cell line comprising C10) said expression cassette or a transgenic plant cell line comprising C11) said recombinant vector; C14 A transgenic plant tissue comprising C9) said nucleic acid molecule or a transgenic plant tissue comprising C10) said expression cassette or a transgenic plant tissue comprising C11) said recombinant vector; C15 A transgenic plant organ comprising C9) said nucleic acid molecule or a transgenic plant organ comprising C10) said expression cassette or a transgenic plant organ comprising C11) said recombinant vector.
  4. 4. A use according to any one of claims 1 to 3, wherein the seed character is selected from at least one of the following: d1 A) the weight of the plant seed, D2 A) the length of the plant seed, D3 A) the width of the plant seed, D4 Plant seed thickness.
  5. 5. A method for improving the grain shape of a seed plant, which method comprises improving the grain shape of the seed plant by inhibiting or reducing or down-regulating the expression of a coding gene comprising said gene encoding a protein according to claim 1 or 2 in a seed plant of interest, said grain shape being grain weight and/or grain length and/or grain width and/or grain thickness.
  6. 6. A method for producing a seed plant with altered seed grain properties, characterized in that the method comprises the step of regulating the expression of a coding gene in a seed plant of interest comprising said coding gene of a protein according to claim 1 or 2, resulting in a seed plant with altered seed grain properties, which are grain weight and/or grain length and/or grain width and/or grain thickness.
  7. 7. The method of claim 6, wherein the modulation is up-regulation or enhancement or elevation.
  8. 8. The method of claim 6, wherein the modulation is down-regulation or inhibition or reduction.
  9. 9. The method of any one of claims 5-8, wherein the seed character is selected from at least one of the following: E1 A) the weight of the plant seed, E2 A) the length of the plant seed, E3 A) the width of the plant seed, E4 Plant seed thickness.
  10. 10. The use according to any one of claims 1 to 4 or the method according to any one of claims 5 to 8, wherein the plant is any one of the following: f1 Dicotyledonous plants, F2 A plant of the order Rosales, F3 A) a leguminous plant, F4 A plant of the genus glycine, F5 Soy bean).

Description

Soybean GmSMS protein and application of encoding gene thereof in regulation and control of grain weight of soybean seeds Technical Field The invention belongs to the field of variation or genetic engineering, and particularly relates to application of soybean GmSMS protein and a coding gene thereof in regulating and controlling the grain weight and grain shape of soybean seeds. Background Soybean (Glycine max) is an important dual-purpose crop for food and feed, and provides rich grease, protein and other nutrient substances for human beings and animals. Therefore, there is a need to cultivate high-yield soybean varieties and increase the soybean yield to meet the demands of soybean consumption and industrial development. Seed weight is a key trait that determines soybean yield, but little is known about the regulatory mechanisms of the process of how soybean plants control the final seed weight. The size and weight of the seeds are precisely controlled by the internal genetic information and environmental signals. Ubiquitin-proteasome pathway, G protein signal, mitogen Activated Protein Kinase (MAPK) signal transduction, transcriptional regulatory factors, and the like control seed development through a variety of complex physiological processes. In recent years, several factors have been identified that regulate the size/weight of soybean seeds. By comparative transcriptomic analysis of cultivated and wild soybean seeds, gmGA20OX was identified as a positive regulator of soybean grain weight. PP2C-1 can interact with GmBZR a and mediate dephosphorylation of GmBZR a, thereby activating brassinosteroid signaling, ultimately regulating seed size/weight. SPINDLY homologous gene GmSSS1 promotes seed enlargement by positively regulating cell expansion and proliferation. ST1 regulates the biosynthetic pathway of pectin by converting UGluA to UGalA, ultimately promoting an increase in hundred weight. POWR1 can regulate seed size by regulating transport of nutrients. GmSWEET10a and GmSWEET b promote an increase in seed size by increasing seed sugar content, and expression of GmSWEET a is regulated by GmST 05. In general, research on soybean grain weight regulation networks is still in the primary stage of gene mining at present, and new genes for regulating soybean grain weight need to be mined and molecular mechanisms thereof need to be analyzed, so that gene resources and theoretical basis are provided for soybean molecular breeding. 14-3-3 Proteins are a family of proteins that are widely found and conserved in eukaryotes. The 14-3-3 protein plays an important role in the growth and development of plants, and participates in stress response, hormone regulation, signal transduction, primary metabolism regulation, gene expression regulation and the like. The 14-3-3 protein participates in regulating the growth and development of plants, including regulating the flowering of plants, affecting the growth of root systems, phototropism of plants, seed development and the like, and the 14-3-3 protein participates in the response of plants to stress, such as drought stress, cold stress, salt stress, biological stress and the like. The method for exploring the influence and the regulation mechanism of the soybean 14-3-3 protein on seed development has important significance and has important value for improving the soybean yield and quality. Disclosure of Invention The application aims to solve the technical problem of how to regulate the plant seed character. Therefore, the invention provides application of the biological material, wherein the application can be application of the biological material in regulating and controlling plant grain traits and/or preparing products for regulating and controlling the plant grain traits, and application of the biological material in plant breeding and/or preparing plant breeding products, and indexes of plant breeding comprise the grain traits. The purpose of the plant breeding includes regulating the grain traits of plants. The biological material is protein, a substance for regulating and controlling the expression of the encoding gene of the protein or a substance for regulating and controlling the content of the protein; The protein is GmSMS and is any one of the following: A1 Amino acid sequence is a protein shown in sequence 2, A2 Protein which is obtained by substituting and/or deleting and/or adding amino acid residues of the protein A1), has more than 80 percent of identity with the protein A1) and can regulate the properties of plant seeds, A3 Fusion proteins obtained by ligating the N-terminal or/and C-terminal of A1) or A2) with a protein tag. The protein can be derived from soybean. Among the above proteins, the protein tag (protein-tag) refers to a polypeptide or protein that is fusion expressed together with a target protein by using a DNA in vitro recombination technique, so as to facilitate the expression, detection, tracing and/or purification of the target pr