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CN-121992004-A - Application of ZmC2DP1 protein in regulation and control of drought resistance and salt tolerance of plants

CN121992004ACN 121992004 ACN121992004 ACN 121992004ACN-121992004-A

Abstract

The invention relates to the technical field of genetic engineering breeding, in particular to application of ZmC2DP1 protein in regulating drought resistance and salt tolerance of plants, wherein the amino acid sequence of the ZmC2DP1 protein is shown as SEQ ID NO.1, and the nucleotide sequence of a gene encoding the ZmC2DP1 protein is shown as SEQ ID NO. 2. After the ZmC2DP1 gene is knocked out, drought resistance and salt tolerance of the mutant plants under drought treatment or salt treatment conditions are improved compared with those of plants in a control group, and the ZmC2DP1 protein is verified to have a negative regulation function on drought resistance and salt tolerance of the plants. Compared with the traditional breeding mode, the method for regulating and controlling the drought resistance and the salt tolerance of the plants has the advantages of short breeding time and strong purposefulness, obviously shortens the period of drought resistance breeding, improves the stress-resistant breeding efficiency of crops, adopts a gene knockout technology to obtain a gene editing homozygous line with improved drought resistance and salt tolerance, provides gene resources for cultivating and improving new varieties of stress-resistant plants, and provides theoretical basis for elucidating the molecular mechanism of ZmC2DP1 protein in the drought resistance and the salt tolerance of the plants.

Inventors

  • QIN FENG
  • YANG ZHIRUI
  • WANG CHEN
  • Zhu tengfei
  • LIU SHENGXUE

Assignees

  • 中国农业大学

Dates

Publication Date
20260508
Application Date
20241107

Claims (10)

  1. The application of ZmC2DP1 protein, its coding gene or biological material containing its coding gene in improving drought resistance of plant is characterized in that the ZmC2DP1 protein is any one of the following proteins: a1 Amino acid sequence is protein of SEQ ID No.1 in a sequence table; A2 Protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown as SEQ ID No.1 in the sequence table.
  2. The application of ZmC2DP1 protein, its coding gene or biological material containing its coding gene in corn salt tolerance, characterized in that the ZmC2DP1 protein is any one of the following proteins: a1 Amino acid sequence is protein of SEQ ID No.1 in a sequence table; A2 Protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown as SEQ ID No.1 in the sequence table.
  3. 3. The use according to claim 1 or 2, characterized in that, The coding gene of the ZmC2DP1 protein is any one of the following: a1 A nucleotide sequence shown as SEQ ID No. 2; A2 The nucleotide sequence shown as SEQ ID No.2 is substituted, deleted and/or added with one or more nucleotides and expresses the same functional protein.
  4. 4. The use according to claim 1 or 2, wherein the biological material is any one of the following B1) to B9): b1 Nucleic acid molecules encoding the ZmC2DP1 proteins described above; B2 An expression cassette comprising the nucleic acid molecule of B1); b3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising the nucleic acid molecule of B1), or a recombinant microorganism comprising the expression cassette of B2), or a recombinant microorganism comprising the recombinant vector of B3); b5 A transgenic plant cell line comprising B1) said nucleic acid molecule, or a transgenic plant cell line comprising B2) said expression cassette, or a transgenic plant cell line comprising B3) said recombinant vector; b6 A) a transgenic plant tissue comprising the nucleic acid molecule of B1), or a transgenic plant tissue comprising the expression cassette of B2), or a transgenic plant tissue comprising the recombinant vector of B3); b7 A transgenic plant organ containing the nucleic acid molecule of B1), or a transgenic plant organ containing the expression cassette of B2), or a transgenic plant organ containing the recombinant vector of B3); b8 A nucleic acid molecule that inhibits or reduces expression of a gene encoding the above protein or a nucleic acid molecule that inhibits or reduces activity of the above protein; b9 An expression cassette, a recombinant vector, a recombinant microorganism or a transgenic plant cell line containing the nucleic acid molecule of B8).
  5. 5. The use according to claim 1 or 2, characterized in that it is effected by inhibiting or reducing the expression and/or activity of the ZmC2DP1 protein in plants.
  6. 6. A method for improving drought resistance of plants is characterized by reducing or inhibiting the expression quantity and/or activity of ZmC2DP1 protein in corn, wherein the ZmC2DP1 protein is any one of the following proteins: a1 Amino acid sequence is protein of SEQ ID No.1 in a sequence table; A2 Protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown as SEQ ID No.1 in the sequence table.
  7. 7. A method for improving salt tolerance of plants is characterized by reducing or inhibiting the expression quantity and/or activity of ZmC2DP1 protein in corn, wherein the ZmC2DP1 protein is any one of the following proteins: a1 Amino acid sequence is protein of SEQ ID No.1 in a sequence table; A2 Protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown as SEQ ID No.1 in the sequence table.
  8. 8. The method according to claim 6 or 7, characterized in that: The inhibition or reduction of the gene expression is achieved by gene knockout or gene silencing.
  9. 9. The method according to claim 6 or 7, characterized in that: Coding genes of the ZmC2DP1 protein are edited in corn through CRISPR/Cas9 technology, the expression quantity and/or activity of the ZmC2DP1 protein in the corn are inhibited, and a gRNA target sequence is shown as SEQ ID No. 9.
  10. 10. The nucleotide sequence of the primer for amplifying the ZmC2DP1 gene is shown as SEQ ID No.3 and SEQ ID No. 4.

Description

Application of ZmC2DP1 protein in regulation and control of drought resistance and salt tolerance of plants Technical Field The invention relates to the technical field of genetic engineering breeding, in particular to application of ZmC2DP1 protein in regulating drought resistance and salt tolerance of plants. Background Researches show that drought is the first major weather disaster which damages agricultural production, and the grain safety problem is particularly important under the current background of rapid global climate change. The cultivation of new main crop varieties with strong stress resistance and stable yield and production increase is an important requirement for guaranteeing the grain safety. Corn is one of the traditional three crops and the corn industry is rapidly developing. However, its production is often affected by different natural disasters. The drought and salt stress resistant genes of the corn identified by the current research are still limited, and the cultivation of drought and salt resistant corn varieties based on molecular design is greatly limited. Therefore, the method has important practical significance in excavating the drought-resistant and salt-tolerant key genes of the corn and analyzing the action mechanism of the key genes. Disclosure of Invention Aiming at the defects existing in the prior art, the invention aims to provide the application of ZmC2DP1 protein in regulating and controlling drought resistance and salt tolerance of plants, and after the ZmC2DP1 gene is knocked out, the resistance of a mutant plant under drought treatment or salt treatment conditions is improved compared with that of a plant in a control group, so that the protein ZmC2DP1 is verified to have a negative regulation function on the drought resistance and the salt tolerance of the plants. In order to solve the technical problems, the technical scheme provided by the invention is as follows: Application of ZmC2DP1 protein, coding gene thereof or biological material containing coding gene thereof in improving drought resistance of plants, wherein the ZmC2DP1 protein is any one of the following proteins: a1 Amino acid sequence is protein of SEQ ID No.1 in a sequence table; A2 Protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown as SEQ ID No.1 in the sequence table. Application of ZmC2DP1 protein, coding gene thereof or biological material containing coding gene thereof in corn salt tolerance, wherein the ZmC2DP1 protein is any one of the following proteins: a1 Amino acid sequence is protein of SEQ ID No.1 in a sequence table; A2 Protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown as SEQ ID No.1 in the sequence table. Preferably, the coding gene of the ZmC2DP1 protein is any one of the following: a1 A nucleotide sequence shown as SEQ ID No. 2; A2 The nucleotide sequence shown as SEQ ID No.2 is substituted, deleted and/or added with one or more nucleotides and expresses the same functional protein. Preferably, in the above application, the substance regulating the expression of the protein-encoding gene or the substance regulating the activity or content of the protein is a biological material, and the biological material may be any one of the following B1) to B9): b1 Nucleic acid molecules encoding the ZmC2DP1 proteins described above; B2 An expression cassette comprising the nucleic acid molecule of B1); b3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising the nucleic acid molecule of B1), or a recombinant microorganism comprising the expression cassette of B2), or a recombinant microorganism comprising the recombinant vector of B3); b5 A transgenic plant cell line comprising B1) said nucleic acid molecule, or a transgenic plant cell line comprising B2) said expression cassette, or a transgenic plant cell line comprising B3) said recombinant vector; b6 A) a transgenic plant tissue comprising the nucleic acid molecule of B1), or a transgenic plant tissue comprising the expression cassette of B2), or a transgenic plant tissue comprising the recombinant vector of B3); b7 A transgenic plant organ containing the nucleic acid molecule of B1), or a transgenic plant organ containing the expression cassette of B2), or a transgenic plant organ containing the recombinant vector of B3); b8 A nucleic acid molecule that inhibits or reduces expression of a gene encoding the above protein or a nucleic acid molecule that inhibits or reduces activity of the above protein; b9 An expression cassette, a recombinant vector, a recombinant microorganism or a transgenic plant cell line containing the nucleic acid molecule of B8). Preferably, this is achieved by inhibiti