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CN-121992005-A - Application of ZmMIK protein in regulation and control of drought resistance and salt tolerance of plants

CN121992005ACN 121992005 ACN121992005 ACN 121992005ACN-121992005-A

Abstract

The invention relates to an application of ZmMIK protein in regulating drought resistance and salt tolerance of plants. The amino acid sequence of ZmMIK protein of the invention is shown as SEQ ID No.1, and the nucleotide sequence of ZmMIK gene encoding ZmMIK protein is shown as SEQ ID No. 2. By knocking out ZmMIK genes, the drought resistance and salt resistance of mutant plants are improved. The invention provides gene resources for cultivating and improving new varieties of stress-resistant plants, and provides theoretical basis for elucidating molecular mechanisms of ZmMIK protein in drought resistance and salt tolerance of plants.

Inventors

  • QIN FENG
  • WANG CHEN
  • YANG ZHIRUI
  • Zhu tengfei
  • LIU SHENGXUE

Assignees

  • 中国农业大学

Dates

Publication Date
20260508
Application Date
20241107

Claims (7)

  1. The zmmik2 gene and the use of a biomaterial comprising the ZmMIK gene in the following directions: Regulating drought resistance and salt tolerance of plants; Genetic breeding of drought resistance and salt tolerance plants; drought resistance and salt tolerance plant germplasm resources are improved; the coding gene sequence of ZmMIK gene is: (1) The coding gene sequence codes the protein shown as SEQ ID No. 1; (2) The nucleotide sequence shown in SEQ ID No.2 is substituted, deleted and/or added with one or more nucleotides and expresses the nucleotide sequence of the protein with the same function as the protein shown in SEQ ID No. 1; the ZmMIK gene-containing biological material includes: b1 The nucleic acid molecule according to item (1) or (2) above; B2 An expression cassette comprising the nucleic acid molecule of B1); b3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising the nucleic acid molecule of B1), or a recombinant microorganism comprising the expression cassette of B2), or a recombinant microorganism comprising the recombinant vector of B3); b5 A transgenic plant cell line comprising B1) said nucleic acid molecule, or a transgenic plant cell line comprising B2) said expression cassette, or a transgenic plant cell line comprising B3) said recombinant vector; b6 A) a transgenic plant tissue comprising the nucleic acid molecule of B1), or a transgenic plant tissue comprising the expression cassette of B2), or a transgenic plant tissue comprising the recombinant vector of B3); b7 A transgenic plant organ containing the nucleic acid molecule of B1), or a transgenic plant organ containing the expression cassette of B2), or a transgenic plant organ containing the recombinant vector of B3); B8 A nucleic acid molecule which inhibits or reduces the expression of the above ZmMIK gene encoding gene or a nucleic acid molecule which inhibits or reduces the activity of a protein encoded by the ZmMIK gene encoding gene; b9 An expression cassette, a recombinant vector, a recombinant microorganism or a transgenic plant cell line containing the nucleic acid molecule of B8).
  2. 2. The use according to claim 1, wherein the use is to knock out the ZmMIK gene in plants to increase drought and salt tolerance of the plants, to seed drought and salt tolerance plants, and to improve drought and salt tolerance plant germplasm resources.
  3. Use of zmmik2 protein in the following directions: Regulating drought resistance and salt tolerance of plants; Genetic breeding of drought resistance and salt tolerance plants; drought resistance and salt tolerance plant germplasm resources are improved; the method is characterized in that the application is as follows: Reducing or inhibiting the level of ZmMIK protein in the plant to increase drought resistance and salt tolerance of the plant; The ZmMIK protein includes: (1) An amino acid sequence shown in SEQ ID No. 1; (2) The protein with the same biological function is obtained by substituting and/or deleting and/or adding one or more amino acid residues in the amino acid sequence shown in SEQ ID No. 1.
  4. 4. A primer for amplifying ZmMIK gene as defined in claim 1, wherein the sequence of the primer is shown as SEQ ID No.3 and SEQ ID No. 4.
  5. 5. A gRNA target is characterized in that the sequence of the gRNA target is shown as SEQ ID No.13, and the gRNA target is used for: inhibiting or reducing the activity of the ZmMIK protein of claim 1; inhibiting or reducing expression of the ZmMIK gene as set forth in claim 1.
  6. 6. A ZmMIK gene mutant is characterized in that the sequence of the ZmMIK gene mutant is shown as SEQ ID No.18, and the ZmMIK gene mutant codes ZmMIK-1 protein shown as SEQ ID No. 19.
  7. 7. A ZmMIK gene mutant is characterized in that the sequence of the ZmMIK gene mutant is shown as SEQ ID No.20, and the ZmMIK gene mutant codes ZmMIK-2 protein shown as SEQ ID No. 21.

Description

Application of ZmMIK protein in regulation and control of drought resistance and salt tolerance of plants Technical Field The invention relates to the technical field of genetic engineering breeding, in particular to application of ZmMIK protein in regulating drought resistance and salt tolerance of plants. Background With global climate change, the emission of greenhouse gases is obviously increased, and the problem of unreasonable fertilization and irrigation in agricultural production is caused, so that extreme weather conditions are frequent, the agricultural ecological environment is deteriorated, and the environmental stresses such as land salinization, drought, extreme change of environmental temperature and the like become the most important factors for limiting the yield and quality of crops. The arid and semiarid agricultural regions in China account for about 52.5% of the land area of the land, wherein the cultivated lands are approximately 8 hundred million mu without irrigation conditions, account for about 5% of the total cultivated lands in China, and are threatened by drought all the year round. Meanwhile, approximately 1/4 of cultivated land in China has salinization with different degrees, and 12-15 hundred million mu of saline-alkali soil exists, wherein 1.5 hundred million mu of saline-alkali soil has development and utilization values. The exploitation of excellent genes and loci of crop stress tolerance and the elucidation of the molecular mechanism of the formation of the traits thereof are important bases for the genetic improvement of crop stress resistance. Corn (Zea mays l.) is one of three main grains, and because of the large plant size, large water demand and sensitivity to environmental stress, it is particularly important to conduct genetic improvement research on stress resistance of corn. The traditional breeding technology has the problems of long period, high blindness, large workload and the like, and related genes are introduced into plants or targets are introduced into plants by a genetic engineering technology method to knock out the related genes so as to improve the stress resistance of crops, so that the technology is mature day by day, and possible gene resources and theoretical basis are provided for the genetic improvement of stress resistance and stable yield of corn. Disclosure of Invention Aiming at the defects in the prior art, the invention aims to provide an application of ZmMIK protein in regulating and controlling drought resistance and salt tolerance of plants. According to the invention, the drought resistance and salt tolerance of corn plants are improved by knocking out the corn ZmMIK gene. In order to achieve the above purpose, the invention adopts the following technical scheme: ZmMIK2 gene and the biological material containing the ZmMIK gene are applied in the following directions: Regulating drought resistance and salt tolerance of plants; Genetic breeding of drought resistance and salt tolerance plants; drought resistance and salt tolerance plant germplasm resources are improved; the coding gene sequence of ZmMIK gene is: (1) The coding gene sequence codes the protein shown as SEQ ID No. 1; (2) The nucleotide sequence shown in SEQ ID No.2 is substituted, deleted and/or added with one or more nucleotides and expresses the nucleotide sequence of the protein with the same function as the protein shown in SEQ ID No. 1; the ZmMIK gene-containing biological material includes: b1 The nucleic acid molecule according to item (1) or (2) above; B2 An expression cassette comprising the nucleic acid molecule of B1); b3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2); B4 A recombinant microorganism comprising the nucleic acid molecule of B1), or a recombinant microorganism comprising the expression cassette of B2), or a recombinant microorganism comprising the recombinant vector of B3); b5 A transgenic plant cell line comprising B1) said nucleic acid molecule, or a transgenic plant cell line comprising B2) said expression cassette, or a transgenic plant cell line comprising B3) said recombinant vector; b6 A) a transgenic plant tissue comprising the nucleic acid molecule of B1), or a transgenic plant tissue comprising the expression cassette of B2), or a transgenic plant tissue comprising the recombinant vector of B3); b7 A transgenic plant organ containing the nucleic acid molecule of B1), or a transgenic plant organ containing the expression cassette of B2), or a transgenic plant organ containing the recombinant vector of B3); B8 A nucleic acid molecule which inhibits or reduces the expression of the above ZmMIK gene encoding gene or a nucleic acid molecule which inhibits or reduces the activity of a protein encoded by the ZmMIK gene encoding gene; b9 An expression cassette, a recombinant vector, a recombinant microorganism or a transgenic plant cell line containing the nucl