Search

CN-121992008-A - Synergistic regulation of yield and alkali-heat tolerance gene ATT2 and application thereof

CN121992008ACN 121992008 ACN121992008 ACN 121992008ACN-121992008-A

Abstract

The invention provides a synergic regulation yield and alkali-heat tolerance gene ATT2 and application thereof. In the present invention, clones were targeted to an ALKALI-heat resistant QTLs, designated ATT2 (alk ali-THERMAL TOLERANCE 2). In normal field conditions, by overexpressing ATT2 (including its promoter and genomic sequences, or its coding sequences), the level of active gibberellin can be increased in moderate amounts, while the yield of grasses can be increased. Under alkaline field conditions, over-expression of ATT2 increases yield under alkaline stress. ATT2 can increase yield under normal conditions and alkali-heat stress.

Inventors

  • LIN HONGXUAN
  • GUO SHUANGQIN
  • CHEN YAXIN
  • SHAN JUNXIANG
  • YE WANGWEI
  • DONG NAIQIAN

Assignees

  • 中国科学院分子植物科学卓越创新中心

Dates

Publication Date
20260508
Application Date
20241108

Claims (12)

  1. 1. A method for improving the characteristics of gramineous plants, which comprises introducing ATT2 genomic DNA or ATT2 coding region genes into gramineous plants, wherein the ATT2 genomic DNA is derived from indica rice, and wherein the improvement of the characteristics of gramineous plants comprises increasing yield, alkali resistance, heat resistance, or plant height.
  2. 2. The method of claim 1, wherein the increasing yield comprises increasing ear length, increasing grain number, increasing seed setting rate, or increasing grain length.
  3. 3. The method of claim 1, wherein introducing the ATT2 genomic DNA or ATT2 coding region gene into the poaceae plant comprises introducing an expression construct or vector comprising the ATT2 genomic DNA or ATT2 coding region gene into the plant.
  4. 4. The method of any one of claims 1 to 3, wherein the ATT2 genomic DNA comprises an ATT2 promoter and a gene region; Preferably, the promoter has a nucleotide sequence shown as SEQ ID NO. 2, a polynucleotide which can be hybridized with the polynucleotide sequence shown as SEQ ID NO. 2 under strict conditions and has the same function of driving expression, or a polynucleotide which has more than 49% of identity with the polynucleotide sequence shown as SEQ ID NO. 2 and has the same function of driving expression; preferably, the gene region of ATT2 genomic DNA has the nucleotide sequence shown in SEQ ID NO. 1 or 3, a polynucleotide which can hybridize with the polynucleotide sequence shown in SEQ ID NO. 1 or 3 under stringent conditions and has the same expression driving function, or a polynucleotide which has more than 49% identity with the polynucleotide sequence shown in SEQ ID NO. 1 or 3 and has the same expression driving function; Preferably, the indica rice includes oryza sativa Long Tepu.
  5. 5. The method according to any one of claims 1 to 3, wherein the ATT2 coding region gene has a nucleotide sequence shown in SEQ ID NO. 3, a polynucleotide capable of hybridizing with the polynucleotide sequence shown in SEQ ID NO. 3 under stringent conditions and having the same function of driving expression, or a polynucleotide having 49% or more of identity with the polynucleotide sequence shown in SEQ ID NO. 3 and having the same function of driving expression.
  6. 6. Use of isolated ATT2 genomic DNA or ATT2 coding region genes or expression constructs or vectors comprising the same, for improving a gramineous plant trait, said ATT2 genomic DNA being derived from indica rice, wherein said improving a gramineous plant trait comprises increasing yield, increasing alkali resistance, increasing heat resistance, or increasing plant height, preferably said increasing yield comprises increasing ear length, increasing ear grain number, increasing seed setting rate, increasing grain length.
  7. 7. The use according to claim 6, wherein the ATT2 genomic DNA comprises an ATT2 promoter and a gene region; Preferably, the promoter has a nucleotide sequence shown as SEQ ID NO. 2, a polynucleotide which can be hybridized with the polynucleotide sequence shown as SEQ ID NO. 2 under strict conditions and has the same function of driving expression, or a polynucleotide which has more than 49% of identity with the polynucleotide sequence shown as SEQ ID NO. 2 and has the same function of driving expression; preferably, the gene region of ATT2 genomic DNA has the nucleotide sequence shown in SEQ ID NO. 1 or 3, a polynucleotide which can hybridize with the polynucleotide sequence shown in SEQ ID NO. 1 or 3 under stringent conditions and has the same expression driving function, or a polynucleotide which has more than 49% identity with the polynucleotide sequence shown in SEQ ID NO. 1 or 3 and has the same expression driving function; Preferably, the ATT2 coding region gene has a nucleotide sequence shown as SEQ ID NO. 3, a polynucleotide which can be hybridized with the polynucleotide sequence shown as SEQ ID NO. 3 under strict conditions and has the same function of driving expression, or a polynucleotide which has more than 49% of identity with the polynucleotide sequence shown as SEQ ID NO. 3 and has the same function of driving expression.
  8. 8. The method according to any one of claims 1 to 5 or the use according to any one of claims 6 to 7, wherein the gramineous plant comprises a cereal plant, or the ATT2 or a homologue thereof is derived from a cereal plant, preferably the gramineous plant comprises a gramineous plant.
  9. 9. The use of ATT2 genomic DNA of a gramineous plant as a molecular marker for identifying a plant trait, wherein the plant trait comprises yield, alkali resistance, heat resistance, or plant height, preferably wherein the yield trait comprises ear length, ear number, seed setting rate, or seed length.
  10. 10. The use according to claim 9, wherein, in identifying the plant trait, the ATT2 genomic DNA of the gramineous plant is analyzed, wherein the ATT2 genomic DNA comprises an ATT2 promoter and gene region, wherein the gramineous plant has an improved trait if the promoter and gene region have the promoter and gene region sequences defined in claim 4, wherein the gramineous plant does not have an improved trait if the promoter and gene region do not have the promoter and gene region sequences defined in claim 4, wherein the improved trait comprises an increased yield, an increased alkali tolerance, an increased heat tolerance, or an increased plant height.
  11. 11. An isolated polynucleotide comprising an ATT2 promoter and gene region, wherein said promoter and gene region has the promoter and gene region sequence defined in claim 4.
  12. 12. A cell, tissue or organ of a grass plant comprising an exogenous ATT2 genomic DNA derived from indica rice having the promoter and gene region sequence defined in claim 4 or an ATT2 coding region gene having the sequence defined in claim 5, or an expression construct or vector containing the same.

Description

Synergistic regulation of yield and alkali-heat tolerance gene ATT2 and application thereof Technical Field The invention belongs to the technical field of botanics, and particularly relates to a synergistic regulation and control yield and alkali-heat tolerance gene ATT2 and application thereof. Background With the challenges of rapid growth and climate change of the global population, improving crop yield and stress tolerance has become an important task for modern agriculture. Especially under abiotic stresses such as salinization and heat stress, it is particularly urgent to improve crop varieties to accommodate these environmental stresses. Data from the national food and agricultural organisation show that over 11 hundred million hectare of cultivated land worldwide is affected by salinization, with 40% of the soil being alkaline. In addition, the global average air temperature has risen by 1.2 ℃ since 1900 years, and it is expected that the next decade will continue to rise, which constitutes a serious threat to crop yield. Cereal crops are important crops, and how to seed more crops on limited cultivated land has been the focus of research by agricultural workers. Research on means for adjusting the plant type of crops and optimizing the planting of the crops is a very important task. Especially, gramineous rice is an important grain crop all the time, and is also one of main foods on dining tables of Chinese people, and the regulation and control of grains is one of important research contents of breeding specialists. Under the background, a novel Green Rice Variety (GRVs) is developed, has stress resistance and high yield, and is important to guaranteeing the future grain safety. There are several factors in rice yield, including effective tillering number, grain number per ear and grain weight. The development of excellent sites for controlling grain types in rice is helpful for better and faster utilization of the sites to cultivate rice germplasm with higher yield. In the field of crop breeding, although some genes have been closely related to stress resistance or yield traits, as the level of demand increases, it is still an aim in the art to find new varieties of plants with genes having specific characteristics and further improved development phenotypes. The alkali-resistant and heat-resistant properties of cereal crops are quantitative properties and are controlled by a plurality of gene loci. The gene locus is not clear in the field, and the control locus is obtained by various research means, so that the alkali-thermal molecular mechanism of crops is clarified, and the construction of alkali-thermal related materials of the crops is facilitated, thereby enhancing the stress tolerance of the crops. Disclosure of Invention The invention aims to provide a gene ATT2 for synergistically regulating and controlling yield and alkali-heat tolerance and application thereof. In a first aspect of the present invention, there is provided a method for improving a gramineous plant trait comprising introducing ATT2 genomic DNA (gATT) or an ATT2 coding region gene into a gramineous plant, said ATT2 genomic DNA being derived from indica rice, wherein said improving the gramineous plant trait comprises increasing yield, increasing alkali resistance, increasing heat resistance, or increasing plant height. In one or more preferred modes, the increased yield includes increased ear length, increased number of kernels per ear (number of kernels per ear), increased seed setting rate or increased kernel length. In one or more preferred modes, the introduction of the ATT2 genomic DNA or ATT2 coding region gene into the grass plant comprises introducing an expression construct or vector comprising the ATT2 genomic DNA or ATT2 coding region gene into the plant. In one or more preferred embodiments, the ATT2 genomic DNA comprises an ATT2 promoter and a gene region (downstream of the promoter). In one or more preferred embodiments, the promoter has the nucleotide sequence shown in SEQ ID NO. 2, a polynucleotide capable of hybridizing to the polynucleotide sequence shown in SEQ ID NO. 2 under stringent conditions and having the same function of driving expression, or a polynucleotide having 49% or more (preferably 50% or more, 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, or 85% or more, more preferably 90% or more, more preferably 95% or more, e.g., 98% or 99% or more) identity to the polynucleotide sequence shown in SEQ ID NO. 2 and having the same function of driving expression. In one or more preferred embodiments, the ATT2 genomic DNA has a nucleotide sequence shown in SEQ ID NO. 1 or 3, a polynucleotide capable of hybridizing to the polynucleotide sequence shown in SEQ ID NO. 1 or 3 under stringent conditions and having the same function of driving expression, or a polynucleotide having 49% or more (preferably 50% or more, 60% or more, 65% or more, 70% or more, 75% or more, 80% or more, or 85%